Western Blot (WB)
(Western blot analysis of lysate from mouse NIH/3T3 cell line, using RBM8A Antibody (Center). MBS9212113 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.)
Rabbit RBM8A Polyclonal Antibody | anti-RBM8A antibody
RBM8A Antibody (Center)
Western Blot (WB)
(Western blot analysis of lysate from mouse NIH/3T3 cell line, using RBM8A Antibody (Center). MBS9212113 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.)
Western Blot (WB)
(Western blot analysis of lysate from mouse NIH/3T3 cell line, using RBM8A Antibody (Center). MBS9212113 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.)
Zhao X.F.,et al.Genomics 63:145-148(2000).
Salicioni A.M.,et al.Genomics 69:54-62(2000).
Kataoka N.,et al.Mol. Cell 6:673-682(2000).
Faurholm B.,et al.Genomics 78:15-18(2001).
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a protein with a conserved RNA-binding motif. The protein is found predominantly in the nucleus, although it is also present in the cytoplasm. It is preferentially associated with mRNAs produced by splicing, including both nuclear mRNAs and newly exported cytoplasmic mRNAs. It is thought that the protein remains associated with spliced mRNAs as a tag to indicate where introns had been present, thus coupling pre- and post-mRNA splicing events. Previously, it was thought that two genes encode this protein, RBM8A and RBM8B; it is now thought that the RBM8B locus is a pseudogene. There are two alternate translation start codons with this gene, which result in two forms of the protein. An allele mutation and a low-frequency noncoding single-nucleotide polymorphism (SNP) in this gene cause thrombocytopenia-absent radius (TAR) syndrome. [provided by RefSeq, Jul 2013]
Uniprot Description
RBM8A: Component of a splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of a few core proteins and several more peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. Core components of the EJC, that remains bound to spliced mRNAs throughout all stages of mRNA metabolism, functions to mark the position of the exon-exon junction in the mature mRNA and thereby influences downstream processes of gene expression including mRNA splicing, nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). The heterodimer MAGOH-RBM8A interacts with PYM that function to enhance the translation of EJC-bearing spliced mRNAs by recruiting them to the ribosomal 48S preinitiation complex. Remains associated with mRNAs in the cytoplasm until the mRNAs engage the translation machinery. Its removal from cytoplasmic mRNAs requires translation initiation from EJC-bearing spliced mRNAs. Associates preferentially with mRNAs produced by splicing. Does not interact with pre-mRNAs, introns, or mRNAs produced from intronless cDNAs. Associates with both nuclear mRNAs and newly exported cytoplasmic mRNAs. Complex with MAGOH is a component of the nonsense mediated decay (NMD) pathway. Belongs to the RBM8A family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: RNA splicing; Translation; RNA-binding; Spliceosome
Chromosomal Location of Human Ortholog: 1q21.1
Cellular Component: nucleoplasm; cytoplasm; nuclear speck; nucleus; cytosol
Molecular Function: mRNA binding; protein binding; RNA binding; nucleotide binding
Biological Process: transcription from RNA polymerase II promoter; regulation of translation; nuclear mRNA splicing, via spliceosome; mRNA export from nucleus; RNA splicing; mRNA catabolic process, nonsense-mediated decay; gene expression; mRNA 3'-end processing; termination of RNA polymerase II transcription; regulation of alternative nuclear mRNA splicing, via spliceosome
Disease: Thrombocytopenia-absent Radius Syndrome
Research Articles on RBM8A
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Product Notes
The RBM8A rbm8a (Catalog #AAA9212113) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RBM8A Antibody (Center) reacts with Mouse (Predicted Reactivity: Xenopus, Bovine, Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's RBM8A can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB~~1:1000. Researchers should empirically determine the suitability of the RBM8A rbm8a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RBM8A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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