Rabbit RBM35A Polyclonal Antibody | anti-RBM35A antibody

RBM35A Antibody

Cat. Num. AAA9404383

• Gene Names: ESRP1; RBM35A; RMB35A
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity chromatography purified via peptide column
• Synonyms: RBM35A; Polyclonal Antibody; RBM35A Antibody; RNA-binding motif protein 35A; RMB35A; Epithelial splicing regulatory protein 1; ESRP1; anti-RBM35A antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: RBM35A antibody is predicted to not cross-react with other RBM35/ESRP family members. At least five isoforms of RBM35A are known to exist; this antibody will detect all five.
• Purity/Purification: Affinity chromatography purified via peptide column
• Form/Format: Supplied in PBS containing 0.02% sodium azide.
• Sequence Length: 677

• Applicable Applications for anti-RBM35A antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Immunogen Type: Peptide
• Immunogen Description: Raised against a 18 amino acid peptide near the amino terminus of human RBM35A.
• Target Name: RBM35A
• Preparation and Storage: Can be stored at -20 degree C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of RBM35A in rat colon tissue lysate with RBM35A antibody at 0.5 ug/mL in (A) the absence and (B) the presence of blocking peptide.)

Immunohistochemistry (IHC)

(Immunohistochemistry of RBM35A in human colon tissue with RBM35A antibody at 2.5 ug/mL.)

Related Product Information for anti-RBM35A antibody

RBM35A, also known as ESRP1, is a mRNA splicing factor that with its related protein RBM35B (ESRP2) are coordinators of an epithelial cell-type-specific splicing program. RBM35A contains three putative RNA recognition motifs and acts by directly binding specific sequences in mRNAs. RBM35A is involved in posttranscriptional regulation of a number of genes such as FGFR2, CD44, CTNND1, and ENAH by exerting a differential effect on protein translation via 5' UTRs of mRNAs. Other recent studies have shown that RMB35A may also act as a novel tumor suppressor.

Product Categories/Family for anti-RBM35A antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 170763525
• NCBI GeneID: 54845
• NCBI Accession #: NP_001030087
• NCBI GenBank Nucleotide #: NM_001034915.2
• Molecular Weight: 73,133 Da
• NCBI Official Full Name: epithelial splicing regulatory protein 1 isoform 2
• NCBI Official Synonym Full Names: epithelial splicing regulatory protein 1
• NCBI Official Symbol: ESRP1
• NCBI Official Synonym Symbols: RBM35A; RMB35A
• NCBI Protein Information: epithelial splicing regulatory protein 1
• UniProt Protein Name: Epithelial splicing regulatory protein 1
• UniProt Gene Name: ESRP1
• UniProt Synonym Gene Names: RBM35A
• UniProt Entry Name: ESRP1_HUMAN

NCBI Description

ESPR1 is an epithelial cell-type-specific splicing regulator (Warzecha et al., 2009 [PubMed 19285943]).[supplied by OMIM, Aug 2009]

Uniprot Description

RBM35A: mRNA splicing factor that regulates the formation of epithelial cell-specific isoforms. Specifically regulates the expression of FGFR2-IIIb, an epithelial cell-specific isoform of FGFR2. Also regulates the splicing of CD44, CTNND1, ENAH, 3 transcripts that undergo changes in splicing during the epithelial-to-mesenchymal transition (EMT). Acts by directly binding specific sequences in mRNAs. Binds the GU-rich sequence motifs in the ISE/ISS-3, a cis-element regulatory region present in the mRNA of FGFR2. Belongs to the ESRP family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: RNA processing

Chromosomal Location of Human Ortholog: 8q22.1

Cellular Component: nucleus

Molecular Function: mRNA binding; nucleotide binding

Biological Process: regulation of RNA splicing; RNA splicing; mRNA processing