Rabbit anti-Human RBM11 Polyclonal Antibody | anti-RBM11 antibody

RBM11, NT (RBM11, Splicing regulator RBM11, RNA-binding motif protein 11) (Azide free) (HRP)

Cat. Num. AAA6340678

• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: RBM11; Polyclonal Antibody; NT (RBM11; Splicing regulator RBM11; RNA-binding motif protein 11) (Azide free) (HRP); RNA-binding motif protein 11; anti-RBM11 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-RBM11 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: RBM11 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 27-54 amino acids from the N-terminal region of human RBM11.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(RBM11 Antibody (N-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the RBM11 antibody detected the RBM11 protein (arrow).)

Related Product Information for anti-RBM11 antibody

RBM11, also known as RNA binding motif protein 11, is a human gene. 2 isoforms of the human protein are produced by alternative splicing.

Product Categories/Family for anti-RBM11 antibody

Antibodies; Binding Proteins

References

Wang, A.G., et al. Biochem. Biophys. Res. Commun. 345(3):1022-1032(2006). Lim, J., et al. Cell 125(4):801-814(2006). Brun, M.E., et al. Gene 312, 41-50 (2003): Gardiner, K., et al. Genomics 79(6):833-843(2002).

NCBI and Uniprot Product Information

• NCBI GI #: 169234630
• NCBI GeneID: 54033
• NCBI Accession #: NP_658983.3
• NCBI GenBank Nucleotide #: NM_144770.3
• UniProt Accession #: P57052; Q6YNC2; Q8NBA1; Q8NFF6
• Molecular Weight: 26,465 Da
• NCBI Official Full Name: splicing regulator RBM11
• NCBI Official Synonym Full Names: RNA binding motif protein 11
• NCBI Official Symbol: RBM11
• NCBI Protein Information: splicing regulator RBM11; RNA-binding motif protein 11; putative RNA-binding protein 11
• UniProt Protein Name: Splicing regulator RBM11
• Protein Family: Splicing regulator
• UniProt Gene Name: RBM11
• UniProt Entry Name: RBM11_HUMAN

Uniprot Description

RBM11: Tissue-specific splicing factor with potential implication in the regulation of alternative splicing during neuron and germ cell differentiation. Antagonizes SRSF1-mediated BCL-X splicing. May affect the choice of alternative 5' splice sites by binding to specific sequences in exons and antagonizing the SR protein SRSF1. 2 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 21q11

Cellular Component: nucleoplasm; nuclear speck; nucleus

Molecular Function: protein homodimerization activity; nucleotide binding; poly(U) binding

Biological Process: multicellular organismal development; RNA splicing; cell differentiation; mRNA processing; regulation of alternative nuclear mRNA splicing, via spliceosome