Rabbit RBBP8 Polyclonal Antibody | anti-RBBP8 antibody

RBBP8 Antibody

Cat. Num. AAA151483

• Gene Names: RBBP8; RIM; COM1; CTIP; JWDS; SAE2; SCKL2
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: RBBP8 Antibody is affinity chromatography purified via peptide column.
• Synonyms: RBBP8; Polyclonal Antibody; RBBP8 Antibody; RIM; COM1; CTIP; JWDS; SAE2; SCKL2; DNA endonuclease RBBP8; CtBP-interacting protein; CtIP; retinoblastoma binding protein 8; anti-RBBP8 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: RBBP8 Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 867

• Applicable Applications for anti-RBBP8 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: RBBP8 antibody can be used for detection of RBBP8 by Western blot at 1 - 2 mug/mL. Antibody can also be used for immunohistochemistry starting at 5 mug/mL. For immunofluorescence start at 20 mug/mL.
• Conjugate: Unconjugated
• Immunogen: RBBP8 antibody was raised against a 14 amino acid synthetic peptide near the center of human RBBP8.
• Buffer: RBBP8 Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: RBBP8 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of RBBP8 in mouse spleen tissue lysate with RBBP8 antibody at (A) 1 and (B) 2 μg/mL.)

Immunohistochemistry (IHC)

(Immunohistochemistry of RBBP8 in rat spleen tissue with RBBP8 antibody at 5 μg/mL.)

Immunofluorescence (IF)

(Immunofluorescence of RBBP8 in rat spleen tissue with RBBP8 antibody at 20 μg/mL.)

Related Product Information for anti-RBBP8 antibody

RBBP8 Antibody: RBBP8, also known as CtBP (carboxy-terminal binding protein) interacting protein (CTIP), was characterized for its role in transcription as a cofactor for the transcriptional repressor CtBP, and also as a binding partner for other proteins including the cell cycle regulators retinoblastoma protein (Rb) and breast cancer 1 (BRCA1). It is ubiquitously expressed and localizes to the nucleus. RBBP8 is thought to modulate the functions in cell proliferation, transcriptional regulation and DNA repair. RBBP8 also plays a central role in the cell cycle checkpoint response to DNA double-stranded breaks (DSBs), with new evidence demonstrating that it controls the choice of DSB repair pathway.

NCBI and Uniprot Product Information

• NCBI GI #: 42718017
• NCBI GeneID: 5932
• NCBI Accession #: NP_976037
• NCBI GenBank Nucleotide #: NM_203292.1
• UniProt Accession #: Q99708; O75371; Q8NHQ3; A6NKN2; A8K8W6; E7ETY1
• Molecular Weight: 98,434 Da
• NCBI Official Full Name: DNA endonuclease RBBP8 isoform b
• NCBI Official Synonym Full Names: retinoblastoma binding protein 8
• NCBI Official Symbol: RBBP8
• NCBI Official Synonym Symbols: RIM; COM1; CTIP; JWDS; SAE2; SCKL2
• NCBI Protein Information: DNA endonuclease RBBP8; RBBP-8; CTBP-interacting protein; sporulation in the absence of SPO11 protein 2 homolog
• UniProt Protein Name: DNA endonuclease RBBP8
• Protein Family: RBBP8 N-terminal-like protein
• UniProt Gene Name: RBBP8
• UniProt Synonym Gene Names: CTIP; CtIP; RBBP-8; RIM; SAE2
• UniProt Entry Name: COM1_HUMAN

NCBI Description

The protein encoded by this gene is a ubiquitously expressed nuclear protein. It is found among several proteins that bind directly to retinoblastoma protein, which regulates cell proliferation. This protein complexes with transcriptional co-repressor CTBP. It is also associated with BRCA1 and is thought to modulate the functions of BRCA1 in transcriptional regulation, DNA repair, and/or cell cycle checkpoint control. It is suggested that this gene may itself be a tumor suppressor acting in the same pathway as BRCA1. Three transcript variants encoding two different isoforms have been found for this gene. More transcript variants exist, but their full-length natures have not been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

CtIP: a transcription factor containing C2H2 zinc fingers. May modulate the functions ascribed to BRCA1 in transcriptional regulation, DNA repair, and/or cell cycle checkpoint control. Interacts with CTBP, with the C-terminal (BRCT) domains of BRCA1, and with the retinoblastoma protein. Abundantly expressed in brain and the immune system and associated with immune system malignancies. Ionizing radiation induces its hyperphosphorylation and dissociation from BRCA1 in an ATM-dependent manner.

Protein type: Transcription, coactivator/corepressor; EC 3.1.-.-

Chromosomal Location of Human Ortholog: 18q11.2

Cellular Component: nucleoplasm; transcriptional repressor complex; nucleolus; nucleus

Molecular Function: protein binding; single-stranded DNA specific endodeoxyribonuclease activity; damaged DNA binding

Biological Process: regulation of transcription from RNA polymerase II promoter; mitosis; meiotic cell cycle; cell division; blastocyst hatching; negative regulation of transcription from RNA polymerase II promoter; DNA repair; G2/M transition DNA damage checkpoint; DNA catabolic process, endonucleolytic; cell cycle checkpoint; double-strand break repair via homologous recombination; G1/S transition of mitotic cell cycle

Disease: Jawad Syndrome; Seckel Syndrome 2