SDS-Page
(CtIP antibody mpAb) tested by Western blot 20 ug of HEK293 whole cell extract was run on SDS-PAGE and probed with antibody at 1 ug/ml.)
Mouse anti-Human CtIP Monoclonal Antibody | anti-CtIP antibody
CtIP antibody (mAb)(Clone 14-1)
Gene Names
RBBP8; RIM; COM1; CTIP; JWDS; SAE2; SCKL2
Reactivity
Human
Applications
Chromatin Immunoprecipitation, Immunoprecipitation, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Western Blot
Purity
Protein A Chromatography
Synonyms
CtIP; Monoclonal Antibody; CtIP antibody (mAb)(Clone 14-1); RBBP8; RIM; COM1; JWDS; SAE2; SCKL2; anti-CtIP antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
14-1
Purity/Purification
Protein A Chromatography
Form/Format
Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Concentration
1ug/ul (varies by lot)
Applicable Applications for anti-CtIP antibody
Chromatin Immunoprecipitation (ChIP), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blot (WB)
Application Notes
Applications Validated:
WB: 1-2ug/ml
WB: 1-2ug/ml
Immunogen
This CtIP antibody was raised against a recombinant protein corresponding to amino acid residues 620-897 of human CtIP.
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
SDS-Page
(CtIP antibody mpAb) tested by Western blot 20 ug of HEK293 whole cell extract was run on SDS-PAGE and probed with antibody at 1 ug/ml.)
SDS-Page
(CtIP antibody mpAb) tested by Western blot 20 ug of HEK293 whole cell extract was run on SDS-PAGE and probed with antibody at 1 ug/ml.)
Related Product Information for anti-CtIP antibody
Short Description: Our CtIP antibody (mAb)(Clone 14-1) was raised in a Mouse host. It has been validated for use in Chromatin Immunoprecipitation, Immunocytochemistry, Immunofluorescence, Immunoprecipitation and Western blot; it has been shown to react with Human samples.
Background: CtIP (CTBP interacting protein, RBBP8, RIM, COM1) is a transcriptional regulator and tumor suppressor first identified through its interaction with CTBP (C-Terminal Binding Protein). CtIP binds to the Rb protein and serves as a transcriptional repressor, through recruitment of CtBP and also by interaction with general transcription factors TFIID and TFIIB. CtIP also interacts with Rb-related protein p130 and Ikaros, a master regulator in lymphocyte development. CtIP is phosphorylated upon DNA damage (likely by ATM and/or ATR), interacts with BRCA1 and plays a critical role in facilitating DNA damage repair. Mutations that abolish the interaction between CtIP and BRCA1 result in loss of cell cycle control leading to the development of cancer.
Background: CtIP (CTBP interacting protein, RBBP8, RIM, COM1) is a transcriptional regulator and tumor suppressor first identified through its interaction with CTBP (C-Terminal Binding Protein). CtIP binds to the Rb protein and serves as a transcriptional repressor, through recruitment of CtBP and also by interaction with general transcription factors TFIID and TFIIB. CtIP also interacts with Rb-related protein p130 and Ikaros, a master regulator in lymphocyte development. CtIP is phosphorylated upon DNA damage (likely by ATM and/or ATR), interacts with BRCA1 and plays a critical role in facilitating DNA damage repair. Mutations that abolish the interaction between CtIP and BRCA1 result in loss of cell cycle control leading to the development of cancer.
Product Categories/Family for anti-CtIP antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
98,434 Da
NCBI Official Full Name
DNA endonuclease RBBP8 isoform a
NCBI Official Synonym Full Names
retinoblastoma binding protein 8
NCBI Official Symbol
RBBP8
NCBI Official Synonym Symbols
RIM; COM1; CTIP; JWDS; SAE2; SCKL2
NCBI Protein Information
DNA endonuclease RBBP8; CTBP-interacting protein; RBBP-8; sporulation in the absence of SPO11 protein 2 homolog
UniProt Protein Name
DNA endonuclease RBBP8
UniProt Gene Name
RBBP8
UniProt Synonym Gene Names
CTIP; CtIP; RBBP-8; RIM; SAE2
UniProt Entry Name
COM1_HUMAN
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Product Notes
The CtIP rbbp8 (Catalog #AAA388779) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The CtIP antibody (mAb)(Clone 14-1) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CtIP can be used in a range of immunoassay formats including, but not limited to, Chromatin Immunoprecipitation (ChIP), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blot (WB). Applications Validated: WB: 1-2ug/ml. Researchers should empirically determine the suitability of the CtIP rbbp8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CtIP, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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