Rabbit anti-Human RBBP7 Polyclonal Antibody | anti-RBBP7 antibody

RBBP7, NT (Nucleosome-remodeling Factor Subunit RBAP46, Histone Acetyltransferase Type B Subunit 2, Retinoblastoma-binding Protein p46, Retinoblastoma-binding Protein 7, RBBP-7, Histone-binding Protein RBBP7, RBAP46) (PE)

Cat. Num. AAA6497409

• Gene Names: RBBP7; RbAp46
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Immunoaffinity chromatography.
• Synonyms: RBBP7; Polyclonal Antibody; NT (Nucleosome-remodeling Factor Subunit RBAP46; Histone Acetyltransferase Type B Subunit 2; Retinoblastoma-binding Protein p46; Retinoblastoma-binding Protein 7; RBBP-7; Histone-binding Protein RBBP7; RBAP46) (PE); anti-RBBP7 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human RBBP7.
• Purity/Purification: Purified by Immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-RBBP7 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-1:500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the N-terminal region of human RBBP7 (KLH).
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of RBBP7 Antibody (N-term) in Hela, MDA-MB231, 293 cell line lysates (35ug/lane). RBBP7 (arrow) was detected using the purified Pab.)

Flow Cytometry (FC/FACS)

(RBBP7 Antibody (N-term) flow cytometry analysis of MDA-MB231 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-RBBP7 antibody

Antibodies; Retinoblastoma Proteins

References

1. Zhang, Y., et.al., Mol. Cell 1(7), 1021-1031 (1998). 2. Verreault, A., et.al., Curr. Biol. 8(2), 96-108 (1998).

NCBI and Uniprot Product Information

• NCBI GI #: 4506439
• NCBI GeneID: 5931
• NCBI Accession #: NP_002884.1
• NCBI GenBank Nucleotide #: NM_002893.3
• UniProt Accession #: Q16576
• Molecular Weight: 47,820 Da
• NCBI Official Full Name: histone-binding protein RBBP7 isoform 2
• NCBI Official Synonym Full Names: retinoblastoma binding protein 7
• NCBI Official Symbol: RBBP7
• NCBI Official Synonym Symbols: RbAp46
• NCBI Protein Information: histone-binding protein RBBP7
• UniProt Protein Name: Histone-binding protein RBBP7
• Protein Family: Histone-binding protein
• UniProt Gene Name: RBBP7
• UniProt Synonym Gene Names: RBAP46; RBBP-7
• UniProt Entry Name: RBBP7_HUMAN

NCBI Description

This protein is a ubiquitously expressed nuclear protein and belongs to a highly conserved subfamily of WD-repeat proteins. It is found among several proteins that binds directly to retinoblastoma protein, which regulates cell proliferation. The encoded protein is found in many histone deacetylase complexes, including mSin3 co-repressor complex. It is also present in protein complexes involved in chromatin assembly. This protein can interact with BRCA1 tumor-suppressor gene and may have a role in the regulation of cell proliferation and differentiation. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2010]