Rabbit anti-Human RB1 Polyclonal Antibody | anti-RB1 antibody

RB1, Phosphorylated (Ser788) (Retinoblastoma-associated Protein, pRb, Rb, PP110, P105-Rb) (APC)

Cat. Num. AAA6497378

• Gene Names: RB1; RB; pRb; OSRC; pp110; p105-Rb; PPP1R130
• Reactivity: Human
• Applications: Dot Blot, Immunohistochemistry
• Purity: Purified by Immunoaffinity chromatography.
• Synonyms: RB1; Polyclonal Antibody; Phosphorylated (Ser788) (Retinoblastoma-associated Protein; pRb; Rb; PP110; P105-Rb) (APC); anti-RB1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human Rb when phosphorylated at Ser788.
• Purity/Purification: Purified by Immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-RB1 antibody: FLISA, Dot Blot (DB), Immunohistochemistry (IHC)
• Application Notes: FLISA: 1:1,000; Dot Blot: 1:500; IHC: 1:50-1:100; Applications are based on unconjugated antibody.
• Immunogen: Synthetic phosphopeptide corresponding to amino acid residues surrounding Ser788 of human Rb (KLH).
• Conjugate: APC
• Antigen Modification: Phosphorylated
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Do not freeze APC conjugates. Light sensitive. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Dot Blot (DB)

(Dot blot analysis of anti-hRb-S788 Phospho-specific Pab on nitrocellulose membrane. 50ng of nonphospho-peptide or phospho-peptide were adsorbed on their respective dots. Antibody working concentration was 0.5ug per ml.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Product Categories/Family for anti-RB1 antibody

Antibodies; Retinoblastoma Proteins

References

1. Wagner, S., et al., Biochem. Pharmacol. 69(7):1059-1067 (2005). 2. Roesch, A., et al., Mod. Pathol. 18(4):565-572 (2005). 3. Lieman, J.H., et al., J. Biol. Chem. 280(11):10484-10490 (2005). 4. Budde, A., et al., Oncogene 24(10):1802-1808 (2005). 5. Zapata, E., et al., FEBS J. 272(6):1343-1353 (2005).

NCBI and Uniprot Product Information

• NCBI GI #: 108773787
• NCBI GeneID: 5925
• NCBI Accession #: NP_000312
• NCBI GenBank Nucleotide #: NM_000321
• UniProt Accession #: P06400; P78499; Q5VW46; Q8IZL4; A8K5E3
• Molecular Weight: 106,159 Da
• NCBI Official Full Name: retinoblastoma-associated protein
• NCBI Official Synonym Full Names: retinoblastoma 1
• NCBI Official Symbol: RB1
• NCBI Official Synonym Symbols: RB; pRb; OSRC; pp110; p105-Rb; PPP1R130
• NCBI Protein Information: retinoblastoma-associated protein; prepro-retinoblastoma-associated protein; protein phosphatase 1, regulatory subunit 130; retinoblastoma suspectibility protein
• UniProt Protein Name: Retinoblastoma-associated protein
• Protein Family: RB1-inducible coiled-coil protein
• UniProt Gene Name: RB1
• UniProt Synonym Gene Names: Rb
• UniProt Entry Name: RB_HUMAN

Uniprot Description

Rb: retinoblastoma tumor suppressor protein regulates cell proliferation by controlling progression through the G1-phase restriction point. Has three distinct binding domains and interacts with regulatory proteins including the E2F family of transcription factors, c-Abl tyrosine kinase and proteins with a conserved LXCXE motif. Cell cycle-dependent phosphorylation by Cdks inhibits Rb target binding, thus allowing cell cycle progression. Recruits and targets histone methyltransferase suv39h1 leading to epigenetic transcriptional repression.

Protein type: Transcription, coactivator/corepressor; Oncoprotein; Nuclear receptor co-regulator; Tumor suppressor

Chromosomal Location of Human Ortholog: 13q14.2

Cellular Component: nucleoplasm; SWI/SNF complex; PML body; Rb-E2F complex; spindle; chromatin; nucleus

Molecular Function: identical protein binding; protein binding; androgen receptor binding; DNA binding; ubiquitin protein ligase binding; transcription coactivator activity; phosphoprotein binding; kinase binding; transcription factor binding; transcription factor activity

Biological Process: sister chromatid biorientation; viral reproduction; positive regulation of transcription, DNA-dependent; negative regulation of transcription from RNA polymerase II promoter, mitotic; regulation of mitotic cell cycle; neuron maturation; positive regulation of macrophage differentiation; cell cycle arrest; neurite development; negative regulation of epithelial cell proliferation; transcription, DNA-dependent; mitotic cell cycle checkpoint; negative regulation of transcription factor activity; regulation of lipid kinase activity; enucleate erythrocyte differentiation; G1/S-specific transcription in mitotic cell cycle; chromatin remodeling; myoblast differentiation; neuron apoptosis; maintenance of mitotic sister chromatid cohesion; gut development; cell division; neuron morphogenesis during differentiation; negative regulation of smoothened signaling pathway; androgen receptor signaling pathway; Ras protein signal transduction; positive regulation of mitotic metaphase/anaphase transition; negative regulation of protein kinase activity; striated muscle cell differentiation; positive regulation of transcription from RNA polymerase II promoter; mitotic cell cycle; negative regulation of transcription, DNA-dependent; cell cycle checkpoint; G1/S transition of mitotic cell cycle

Disease: Bladder Cancer; Osteogenic Sarcoma; Small Cell Cancer Of The Lung; Retinoblastoma