anti-Human, Rat MKK3/6 Monoclonal Antibody | anti-MKK3/6 antibody

Anti-MKK3/6 Mouse mAb

Cat. Num. AAA475429

• Gene Names: MAP2K6; MEK6; MKK6; MAPKK6; PRKMK6; SAPKK3; SAPKK-3
• Reactivity: Human, Rat
• Applications: Western Blot, Immunoprecipitation
• Purity: Affinity Purified
• Synonyms: MKK3/6; Monoclonal Antibody; Anti-MKK3/6 Mouse mAb; AW212142; dual specificity mitogen activated protein kinase kinase 3; Dual specificity mitogen activated protein kinase kinase 6; Dual specificity mitogen-activated protein kinase kinase 3; MAP kinase kinase 3; MAP kinase kinase 6; map2k3; MAP2K6; MAPK ERK kinase 3; MAPK/ERK kinase 3; MAPK/ERK kinase 6; MAPKK 3; MAPKK 6; MAPKK3; MAPKK6; MEK 3; MEK 6; MEK3; Mitogen activated protein kinase kinase 3; Mitogen activated protein kinase kinase 6; MKK 3; MKK3; MKK6; mMKK3b; MP2K3_HUMAN; PRKMK 3; PRKMK3; PRKMK6; Protein kinase; mitogen activated; kinase 6 (MAP kinase kinase 6); protein kinase; mitogen-activated; kinase 3; SAPK kinase 2; SAPKK 2; SAPKK 3; SAPKK-2; SAPKK2; SAPKK3; Stress activated protein kinase kinase 2; Stress activated protein kinase kinase 3; Stress-activated protein kinase kinase 2; anti-MKK3/6 antibody

• Host: Mouse; Source: Human
• Reactivity: Human, Rat
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 8A11-G11-G11
• Purity/Purification: Affinity Purified
• Form/Format: Purified mouse monoclonal antibody in PBS (pH 7.4) containing with 0.03% Proclin300 and 50% glycerol.
• Concentration: 3.2mg/ml (varies by lot)
• Sequence Length: 334

• Applicable Applications for anti-MKK3/6 antibody: Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: WB: 1:1000
• Immunogen: Purified recombinant human MKK6 protein fragments expressed in E Coli.
• Sensitivity: This antibody detects endogenous levels of MKK3 and MKK6 and does not cross-react with related proteins.
• Sequence Similarities: Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
• Subunit Structure: MKK3:Binds to DYRK1B/MIRK and increases its kinase activity. Part of a complex with MAP3K3, RAC1 and CCM2. Interacts with ARRB1. Interacts with Yersinia yopJ. MKK6: Dimer. Interacts with Yersinia yopJ. Interacts (via its D domain) with its substrates MAPK11, MAPK12, MAPK13 and MAPK14 (By similarity). Interacts (via its DVD domain) with MAP3Ks activators like MAP3K5/ASK1, MAP3K1/MEKK1, MAP3K2/MEKK2, MAP3K3/MEKK3, MAP3K4/MEKK4, MAP3K7/TAK1, MAP3K11/MLK3 and MAP3K17/TAOK2 (By similarity). Interacts with DCTN1. Interacts with EIF2AK2/PKR.
• Subcellular Location: Cytoplasm ; Nucleus

Western Blot (WB)

(Western blot analysis of extracts from PC12, C6, Ramos, Jurkat and Hela cell lysates using MKK3/6 mouse mAb (1:1000 diluted).Predicted band size:40KDa.Observed band size:40KDa.)

Immunoprecipitation (IP)

(Immunoprecipitation analysis of Hela cell lysates using MKK3/6 mouse mAb.)

Related Product Information for anti-MKK3/6 antibody

Entrez Summary: This gene encodes a member of the dual specificity protein kinase family, which functions as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein phosphorylates and activates p38 MAP kinase in response to inflammatory cytokines or environmental stress. As an essential component of p38 MAP kinase mediated signal transduction pathway, this gene is involved in many cellular processes such as stress induced cell cycle arrest, transcription activation and apoptosis.
UniPort Summary Function: Dual specificity kinase. Is activated by cytokines and environmental stress in vivo. Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the MAP kinase p38.

NCBI and Uniprot Product Information

• NCBI GI #: 14589900
• NCBI GeneID: 5608
• NCBI Accession #: NP_002749.2
• NCBI GenBank Nucleotide #: NM_002758
• UniProt Accession #: P52564
• Molecular Weight: 40
• NCBI Official Full Name: dual specificity mitogen-activated protein kinase kinase 6 isoform 1
• NCBI Official Synonym Full Names: mitogen-activated protein kinase kinase 6
• NCBI Official Symbol: MAP2K6
• NCBI Official Synonym Symbols: MEK6; MKK6; MAPKK6; PRKMK6; SAPKK3; SAPKK-3
• NCBI Protein Information: dual specificity mitogen-activated protein kinase kinase 6
• UniProt Protein Name: Dual specificity mitogen-activated protein kinase kinase 6
• UniProt Gene Name: MAP2K6
• UniProt Synonym Gene Names: MEK6; MKK6; PRKMK6; SKK3; MAP kinase kinase 6; MAPKK 6; MEK 6; SAPK kinase 3; SAPKK-3; SAPKK3

NCBI Description

This gene encodes a member of the dual specificity protein kinase family, which functions as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein phosphorylates and activates p38 MAP kinase in response to inflammatory cytokines or environmental stress. As an essential component of p38 MAP kinase mediated signal transduction pathway, this gene is involved in many cellular processes such as stress induced cell cycle arrest, transcription activation and apoptosis. [provided by RefSeq, Jul 2008]

Uniprot Description

MKK6: a dual-specificity protein kinase of the STE7 family. Activates p38 MAP kinase by phosphorylating a Thr and a Tyr residue in the activation loop. Is activated by cytokines and environmental stress in vivo. Three alternatively spliced isoforms have been reported.

Protein type: EC 2.7.12.2; Kinase, protein; Protein kinase, STE; Protein kinase, dual-specificity (non-receptor); STE group; STE7 family

Chromosomal Location of Human Ortholog: 17q24.3

Cellular Component: cytoplasm; cytosol; nucleoplasm

Molecular Function: MAP kinase kinase activity; protein binding; protein kinase binding; receptor signaling protein serine/threonine kinase activity

Biological Process: activation of MAPK activity; cell cycle arrest; DNA damage induced protein phosphorylation; signal transduction