Rabbit anti-Human RASGRP4 Polyclonal Antibody | anti-RASGRP4 antibody

RASGRP4 antibody

Cat. Num. AAA9413519

• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Purified by antigen-affinity chromatography.
• Synonyms: RASGRP4; Polyclonal Antibody; RASGRP4 antibody; anti-RASGRP4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Purity/Purification: Purified by antigen-affinity chromatography.
• Form/Format: Supplied in 0.1M Tris-buffered saline with 10% Glycerol (pH7.0). 0.01% Thimerosal was added as a preservative.
• Sequence Length: 673

• Applicable Applications for anti-RASGRP4 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: Western blotting: 1:500-1:3000; Immunohistochemistry: 1:100-1:250; Immunofluorescence: 1:100-1:200
• Immunogen Type: Peptide
• Immunogen Description: Synthetic peptide contain a sequence corresponding to a region within amino acids 108 and 170 of Human RASGRP4
• Target Name: RASGRP4
• Preparation and Storage: Store at -20 degree C for long term preservation (recommended). Store at 4 degree C for short term use.

Testing Data

(Sample (30 ug of whole cell lysate) A: Jurkat 7.5% SDS PAGE Primary antibody diluted at 1: 1000)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using RASGRP4 antibody at 1: 100 dilution.)

Immunofluorescence (IF)

(Immunofluorescence analysis of methanol-fixed HeLa, using RASGRP4 antibody at 1: 500 dilution.)

Related Product Information for anti-RASGRP4 antibody

The protein encoded by this gene is a member of the Ras guanyl nucleotide-releasing protein (RasGRP) family of Ras guanine nucleotide exchange factors. It contains a Ras exchange motif, a diacylglycerol-binding domain, and two calcium-binding EF hands. This protein was shown to activate H-Ras in a cation-dependent manner in vitro. Expression of this protein in myeloid cell lines was found to be correlated with elevated level of activated RAS protein, and the RAS activation can be greatly enhanced by phorbol ester treatment, which suggested a role of this protein in diacylglycerol regulated cell signaling pathways. Studies of a mast cell leukemia cell line expressing substantial amounts of abnormal transcripts of this gene indicated that this gene may play an important role in the final stages of mast cell development. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Product Categories/Family for anti-RASGRP4 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 26051258
• NCBI GeneID: 115727
• NCBI Accession #: NP_733749
• NCBI GenBank Nucleotide #: NM_170604.2
• Molecular Weight: 66,972 Da
• NCBI Official Full Name: RAS guanyl-releasing protein 4 isoform a
• NCBI Official Synonym Full Names: RAS guanyl releasing protein 4
• NCBI Official Symbol: RASGRP4
• NCBI Protein Information: RAS guanyl-releasing protein 4
• UniProt Protein Name: RAS guanyl-releasing protein 4
• Protein Family: RAS guanyl-releasing protein
• UniProt Gene Name: RASGRP4
• UniProt Entry Name: GRP4_HUMAN

NCBI Description

The protein encoded by this gene is a member of the Ras guanyl nucleotide-releasing protein (RasGRP) family of Ras guanine nucleotide exchange factors. It contains a Ras exchange motif, a diacylglycerol-binding domain, and two calcium-binding EF hands. This protein was shown to activate H-Ras in a cation-dependent manner in vitro. Expression of this protein in myeloid cell lines was found to be correlated with elevated level of activated RAS protein, and the RAS activation can be greatly enhanced by phorbol ester treatment, which suggested a role of this protein in diacylglycerol regulated cell signaling pathways. Studies of a mast cell leukemia cell line expressing substantial amounts of abnormal transcripts of this gene indicated that this gene may play an important role in the final stages of mast cell development. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2009]

Uniprot Description

RASGRP4: Functions as a cation- and diacylglycerol (DAG)- regulated nucleotide exchange factor activating Ras through the exchange of bound GDP for GTP. May function in mast cells differentiation. Belongs to the RASGRP family. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: GAPs; GAPs, Ras

Chromosomal Location of Human Ortholog: 19q13.1

Cellular Component: membrane; cytoplasm; plasma membrane

Molecular Function: diacylglycerol binding; GTP-dependent protein binding; calcium ion binding; Ras guanyl-nucleotide exchange factor activity

Biological Process: cell proliferation; phospholipase C activation; response to extracellular stimulus; small GTPase mediated signal transduction; myeloid cell differentiation; positive regulation of Ras protein signal transduction; cell growth; transmembrane receptor protein tyrosine kinase signaling pathway; regulation of G-protein coupled receptor protein signaling pathway