Western Blot (WB)
(Western Blot detection against Immunogen (37.11kD).)
Mouse anti-Human RASGRP4 Monoclonal Antibody | anti-RASGRP4 antibody
RASGRP4 (RAS Guanyl-releasing Protein 4)
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Dilution: Immunohistochemistry (Formalin fixed paraffin embedded): 3ug/ml
Western Blot (WB)
(Western Blot detection against Immunogen (37.11kD).)
Western Blot (WB)
(Western Blot detection against Immunogen (37.11kD).)
Immunohistochemistry (IHC)
(Immunoperoxidase of monoclonal antibody to RASGRP4 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 3ug/ml].)
Immunohistochemistry (IHC)
(Immunoperoxidase of monoclonal antibody to RASGRP4 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 3ug/ml].)
Testing Data
(Detection limit for recombinant GST tagged RASGRP4 is ~0.3ng/ml as a capture antibody.)
Testing Data
(Detection limit for recombinant GST tagged RASGRP4 is ~0.3ng/ml as a capture antibody.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a member of the Ras guanyl nucleotide-releasing protein (RasGRP) family of Ras guanine nucleotide exchange factors. It contains a Ras exchange motif, a diacylglycerol-binding domain, and two calcium-binding EF hands. This protein was shown to activate H-Ras in a cation-dependent manner in vitro. Expression of this protein in myeloid cell lines was found to be correlated with elevated level of activated RAS protein, and the RAS activation can be greatly enhanced by phorbol ester treatment, which suggested a role of this protein in diacylglycerol regulated cell signaling pathways. Studies of a mast cell leukemia cell line expressing substantial amounts of abnormal transcripts of this gene indicated that this gene may play an important role in the final stages of mast cell development. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2009]
Uniprot Description
RASGRP4: Functions as a cation- and diacylglycerol (DAG)- regulated nucleotide exchange factor activating Ras through the exchange of bound GDP for GTP. May function in mast cells differentiation. Belongs to the RASGRP family. 6 isoforms of the human protein are produced by alternative splicing.
Protein type: GAPs; GAPs, Ras
Chromosomal Location of Human Ortholog: 19q13.1
Cellular Component: membrane; cytoplasm; plasma membrane
Molecular Function: diacylglycerol binding; GTP-dependent protein binding; calcium ion binding; Ras guanyl-nucleotide exchange factor activity
Biological Process: cell proliferation; phospholipase C activation; small GTPase mediated signal transduction; response to extracellular stimulus; myeloid cell differentiation; positive regulation of Ras protein signal transduction; cell growth; regulation of G-protein coupled receptor protein signaling pathway; transmembrane receptor protein tyrosine kinase signaling pathway
Research Articles on RASGRP4
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Product Notes
The RASGRP4 rasgrp4 (Catalog #AAA643583) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The RASGRP4 (RAS Guanyl-releasing Protein 4) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's RASGRP4 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC). Suitable for use in ELISA, Western Blot and Immunohistochemistry. Dilution: Immunohistochemistry (Formalin fixed paraffin embedded): 3ug/ml. Researchers should empirically determine the suitability of the RASGRP4 rasgrp4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: CGLCCHKHCR DQVKVECKKR PGAKGDAGPP GAPVPSTPAP HASCGSEENH SYTLSLEPET GCQLRHAWTQ TESPHPSWET DTVPCPVMDP PSTASSKLDS. It is sometimes possible for the material contained within the vial of "RASGRP4, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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