Rabbit anti-Human RASGRP1 Polyclonal Antibody | anti-RASGRP1 antibody

RASGRP1, ID (RASGRP1, RASGRP, RAS guanyl-releasing protein 1, Calcium and DAG-regulated guanine nucleotide exchange factor II, Ras guanyl-releasing protein) (Biotin)

Cat. Num. AAA6340462

• Gene Names: RASGRP1; V; RASGRP; hRasGRP1; CALDAG-GEFI; CALDAG-GEFII
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, Flow Cytometry, Functional Assay
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: RASGRP1; Polyclonal Antibody; ID (RASGRP1; RASGRP; RAS guanyl-releasing protein 1; Calcium and DAG-regulated guanine nucleotide exchange factor II; Ras guanyl-releasing protein) (Biotin); Ras guanyl-releasing protein; anti-RASGRP1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human RASGRP1.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-RASGRP1 antibody: Immunohistochemistry (IHC) Paraffin, Western Blot (WB), Flow Cytometry (FC/FACS), FLISA
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 495-521 in the central region of human RASGRP1, Swiss/UniProt Accession: O95267. Species sequence homology: mouse and rat
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in HepG2 cell line lysates (35ug/lane) usingMBS642452. This demonstrates that MBS642452 detected the RASGRP1 protein (arrow).)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue using MBS642452 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS642452 for immunohistochemistry.)

Flow Cytometry (FC/FACS)

(Flow Cytometry analysis of HepG2 cells using MBS642452 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-RASGRP1 antibody

Antibodies; GTPase; Rab; Ras; Rho Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 190684644
• NCBI GeneID: 10125
• NCBI Accession #: NP_005730.2; NP_001122074.1
• NCBI GenBank Nucleotide #: NM_005739.3
• UniProt Accession #: O95267; Q9R1K8; Q9Z1S3
• Molecular Weight: 62,674 Da
• NCBI Official Full Name: RAS guanyl-releasing protein 1 isoform a
• NCBI Official Synonym Full Names: RAS guanyl releasing protein 1
• NCBI Official Symbol: RASGRP1
• NCBI Official Synonym Symbols: V; RASGRP; hRasGRP1; CALDAG-GEFI; CALDAG-GEFII
• NCBI Protein Information: RAS guanyl-releasing protein 1
• UniProt Protein Name: RAS guanyl-releasing protein 1
• Protein Family: RAS guanyl-releasing protein
• UniProt Gene Name: RASGRP1
• UniProt Synonym Gene Names: RASGRP; CalDAG-GEFII
• UniProt Entry Name: GRP1_HUMAN

NCBI Description

This gene is a member of a family of genes characterized by the presence of a Ras superfamily guanine nucleotide exchange factor (GEF) domain. It functions as a diacylglycerol (DAG)-regulated nucleotide exchange factor specifically activating Ras through the exchange of bound GDP for GTP. It activates the Erk/MAP kinase cascade and regulates T-cells and B-cells development, homeostasis and differentiation. Alternatively spliced transcript variants encoding different isoforms have been identified. Altered expression of the different isoforms of this protein may be a cause of susceptibility to systemic lupus erythematosus (SLE). [provided by RefSeq, Jul 2008]

Uniprot Description

RASGRP1: Functions as a diacylglycerol (DAG)-regulated nucleotide exchange factor specifically activating Ras through the exchange of bound GDP for GTP. Activates the Erk/MAP kinase cascade. Couples T-lymphocytes and B-lymphocytes antigen receptors to the activation of Ras. Hence, regulates T-cells and B-cells development, homeostasis and differentiation. Functions also in FcERI-evoked degranulation and cytokine secretion by mast cells, regulating allergic responses. May also function in other cell types differentiation. Defects in RASGRP1 may contribute to susceptibility to systemic lupus erythematosus (SLE). SLE is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. SLE is thought to represent a failure of the regulatory mechanisms of the autoimmune system. Aberrantly spliced isoforms and/or diminished levels of RASGRP1 are found in a cohort of SLE patients raising the possibility that dysregulation of this signaling protein contributes to the development of autoimmunity in a subset of SLE patients. Belongs to the RASGRP family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: GEFs, Ras; GEFs

Chromosomal Location of Human Ortholog: 15q14

Cellular Component: Golgi membrane; endoplasmic reticulum membrane; mast cell granule; membrane; plasma membrane; cytosol

Molecular Function: guanyl-nucleotide exchange factor activity; calcium ion binding; lipid binding

Biological Process: platelet activation; inflammatory response to antigenic stimulus; Ras protein signal transduction; regulation of phosphoinositide 3-kinase cascade; vesicle transport along microtubule; innate immune response; cytokine production; mast cell degranulation; blood coagulation; signal transduction; cell differentiation; secretory granule localization