Typical Testing Data/Standard Curve (for reference only)
General LTB4 ELISA Kit | LTB4 elisa kit
LTB4 (Leukotriene B4) ELISA Kit
Gene Names
LTB4R2; BLT2; NOP9; BLTR2; JULF2; KPG_004; LTB4-R2; LTB4-R 2
Reactivity
General
Synonyms
LTB4; LTB4 (Leukotriene B4) ELISA Kit; LTB4 elisa kit
Reactivity
General
Specificity
This kit recognizes LTB4 in samples. No significant cross-reactivity or interference between LTB4 and analogues was observed.
Sequence Length
358
Samples
Serum, plasma and other biological fluids
Assay Type
Quantitative Competitive
Detection Range
15.63-1000pg/mL
Sensitivity
9.38pg/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level LTB4 were tested 20 times on one plate, respectively.
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level LTB4 were tested on 3 different plates, 20 replicates in each plate.
Preparation and Storage
Store at 4 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for LTB4 elisa kit
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of LTB4 concentrations in serum, plasma and other biological fluids.
Principle of the Assay: This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with LTB4. During the reaction, LTB4 in the sample or standard competes with a fixed amount of LTB4 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to LTB4. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of LTB4 in the samples is then determined by comparing the OD of the samples to the standard curve.
Principle of the Assay: This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with LTB4. During the reaction, LTB4 in the sample or standard competes with a fixed amount of LTB4 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to LTB4. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of LTB4 in the samples is then determined by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
37,942 Da
NCBI Official Full Name
LTB4 receptor JULF2
NCBI Official Synonym Full Names
leukotriene B4 receptor 2
NCBI Official Symbol
LTB4R2
NCBI Official Synonym Symbols
BLT2; NOP9; BLTR2; JULF2; KPG_004; LTB4-R2; LTB4-R 2
NCBI Protein Information
leukotriene B4 receptor 2; LTB4 receptor JULF2; leukotriene B4 receptor BLT2; seven transmembrane receptor BLTR2
UniProt Protein Name
Leukotriene B4 receptor 2
UniProt Gene Name
LTB4R2
UniProt Synonym Gene Names
BLT2R; BLTR2; LTB4-R 2; LTB4-R2
UniProt Entry Name
LT4R2_HUMAN
Uniprot Description
LTB4R2: Low-affinity receptor for leukotrienes including leukotriene B4. Mediates chemotaxis of granulocytes and macrophages. The response is mediated via G-proteins that activate a phosphatidylinositol-calcium second messenger system. The rank order of affinities for the leukotrienes is LTB4 > 12-epi-LTB4 > LTB5 > LTB3. Belongs to the G-protein coupled receptor 1 family.
Protein type: Motility/polarity/chemotaxis; GPCR, family 1; Membrane protein, integral; Membrane protein, multi-pass; Receptor, GPCR
Chromosomal Location of Human Ortholog: 14q12
Cellular Component: membrane; integral to plasma membrane; plasma membrane
Molecular Function: leukotriene B4 receptor activity; leukotriene receptor activity
Biological Process: G-protein coupled receptor protein signaling pathway; negative regulation of adenylate cyclase activity; chemotaxis; keratinocyte migration
Research Articles on LTB4
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Product Notes
The General LTB4 ltb4r2 (Catalog #AAA2515771) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2515771 ELISA Kit recognizes General LTB4. It is sometimes possible for the material contained within the vial of "LTB4, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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