Western Blot (WB)
(Western blot analysis of extracts of mouse brain, using RASGRF1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)
Rabbit anti-Human, Mouse RASGRF1 Polyclonal Antibody | anti-RASGRF1 antibody
RASGRF1 Polyclonal Antibody
IHC: 1:50 - 1:200
IF: 1:50 - 1:100
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Western Blot (WB)
(Western blot analysis of extracts of mouse brain, using RASGRF1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)
Western Blot (WB)
(Western blot analysis of extracts of mouse brain, using RASGRF1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human stomach using RASGRF1 antibody at dilution of 1:100 (40x lens).)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human stomach using RASGRF1 antibody at dilution of 1:100 (40x lens).)
Immunofluorescence (IF)
(Immunofluorescence analysis of U2OS cells using RASGRF1 antibody. Blue: DAPI for nuclear staining.)
Immunofluorescence (IF)
(Immunofluorescence analysis of U2OS cells using RASGRF1 antibody. Blue: DAPI for nuclear staining.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a guanine nucleotide exchange factor (GEF) similar to the Saccharomyces cerevisiae CDC25 gene product. Functional analysis has demonstrated that this protein stimulates the dissociation of GDP from RAS protein. The studies of the similar gene in mouse suggested that the Ras-GEF activity of this protein in brain can be activated by Ca2+ influx, muscarinic receptors, and G protein beta-gamma subunit. Mouse studies also indicated that the Ras-GEF signaling pathway mediated by this protein may be important for long-term memory. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Mar 2009]
Uniprot Description
RASGRF1: a guanine nucleotide exchange factor (GEF). Stimulates the dissociation of GDP from RAS protein. Activated by Ca2+ influx, muscarinic receptors, and G protein beta-gamma subunit in the mouse brain,. The signaling pathway mediated by this protein may be important for long-term memory. Two alternatively spliced variants have been reported.
Protein type: GEFs, Ras; GEFs
Chromosomal Location of Human Ortholog: 15q24.2
Cellular Component: neuron projection; growth cone; plasma membrane; cytosol
Molecular Function: Rho guanyl-nucleotide exchange factor activity; glutamate receptor binding; guanyl-nucleotide exchange factor activity; Ras guanyl-nucleotide exchange factor activity
Biological Process: regulation of Ras protein signal transduction; regulation of Rac protein signal transduction; synaptic transmission; cell proliferation; long-term memory; small GTPase mediated signal transduction; regulation of synaptic plasticity; positive regulation of Ras protein signal transduction; signal transduction; neurite development; regulation of neuronal synaptic plasticity
Research Articles on RASGRF1
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Product Notes
The RASGRF1 rasgrf1 (Catalog #AAA9125548) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RASGRF1 Polyclonal Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's RASGRF1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). WB: 1:500 - 1:2000 IHC: 1:50 - 1:200 IF: 1:50 - 1:100. Researchers should empirically determine the suitability of the RASGRF1 rasgrf1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RASGRF1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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