Rabbit RAS Polyclonal Antibody | anti-RAS antibody

RAS Antibody

Cat. Num. AAA9413118

• Gene Names: HRAS; CTLO; HAMSV; HRAS1; RASH1; p21ras; C-H-RAS; H-RASIDX; C-BAS/HAS; C-HA-RAS1
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot
• Synonyms: RAS; Polyclonal Antibody; RAS Antibody; CTLO; HAMSV; HRAS1; K-RAS; N-RAS; RASH1; C-H-RAS; H-RASIDX; C-BAS/HAS; C-HA-RAS1; anti-RAS antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of total HRAS protein.
• Form/Format: Supplied at 0.7mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.3, 0.05% sodium azide and 50% glycerol.
• Sequence Length: 170

• Applicable Applications for anti-RAS antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: ELISA: 1:1000-1:5000; Western blotting: 1:200-1:1000
• Immunogen Type: Peptide
• Immunogen Description: Synthetic peptide corresponding to a region derived from 60-74 amino acids of Human v-Ha-ras Harvey rat sarcoma viral oncogene homolog
• Target Name: HRAS
• Preparation and Storage: Store at -20 degree C/1 year

Testing Data

(Gel: 10%SDS-PAGELane1: Hela cell lysateLane2: HepG2 cell lysateLane3: 231 cell lysateLysates: 30ug per lanePrimary antibody: 1/400 dilutionSecondary antibody: Donkey anti Rabbit IgG - H&L (HRP) at 1/3000 dilutionExposure time: 20 seconds)

Related Product Information for anti-RAS antibody

This gene belongs to the Ras oncogene family, whose members are related to the transforming genes of mammalian sarcoma retroviruses. The products encoded by these genes function in signal transduction pathways. These proteins can bind GTP and GDP, and they have intrinsic GTPase activity. This protein undergoes a continuous cycle of de- and re-palmitoylation, which regulates its rapid exchange between the plasma membrane and the Golgi apparatus. Mutations in this gene cause Costello syndrome, a disease characterized by increased growth at the prenatal stage, growth deficiency at the postnatal stage, predisposition to tumor formation, mental retardation, skin and musculoskeletal abnormalities, distinctive facial appearance and cardiovascular abnormalities. Defects in this gene are implicated in a variety of cancers, including bladder cancer, follicular thyroid cancer, and oral squamous cell carcinoma. Multiple transcript variants, which encode different isoforms, have been identified for this gene.

Product Categories/Family for anti-RAS antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 34222246
• NCBI GeneID: 3265
• NCBI Accession #: NP_789765
• NCBI GenBank Nucleotide #: NM_176795.3
• Molecular Weight: 18,870 Da
• NCBI Official Full Name: GTPase HRas isoform 2
• NCBI Official Synonym Full Names: Harvey rat sarcoma viral oncogene homolog
• NCBI Official Symbol: HRAS
• NCBI Official Synonym Symbols: CTLO; HAMSV; HRAS1; RASH1; p21ras; C-H-RAS; H-RASIDX; C-BAS/HAS; C-HA-RAS1
• NCBI Protein Information: GTPase HRas
• UniProt Protein Name: GTPase HRas
• Protein Family: Ras protein
• UniProt Gene Name: HRAS
• UniProt Synonym Gene Names: HRAS1
• UniProt Entry Name: RASH_HUMAN

NCBI Description

This gene belongs to the Ras oncogene family, whose members are related to the transforming genes of mammalian sarcoma retroviruses. The products encoded by these genes function in signal transduction pathways. These proteins can bind GTP and GDP, and they have intrinsic GTPase activity. This protein undergoes a continuous cycle of de- and re-palmitoylation, which regulates its rapid exchange between the plasma membrane and the Golgi apparatus. Mutations in this gene cause Costello syndrome, a disease characterized by increased growth at the prenatal stage, growth deficiency at the postnatal stage, predisposition to tumor formation, mental retardation, skin and musculoskeletal abnormalities, distinctive facial appearance and cardiovascular abnormalities. Defects in this gene are implicated in a variety of cancers, including bladder cancer, follicular thyroid cancer, and oral squamous cell carcinoma. Multiple transcript variants, which encode different isoforms, have been identified for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

HRas: a small GTPase protein of the Ras family. Alternates between an inactive form bound to GDP and an active form bound to GTP. Activated by a guanine nucleotide-exchange factor (GEF) and inactivated by a GTPase-activating protein (GAP). Mutations are implicated in a variety of human tumors.

Protein type: G protein; Motility/polarity/chemotaxis; Oncoprotein; G protein, monomeric; G protein, monomeric, Ras

Chromosomal Location of Human Ortholog: 11p15.5

Cellular Component: cytoplasm; cytosol; Golgi apparatus; Golgi membrane; nucleus; perinuclear region of cytoplasm; plasma membrane

Molecular Function: GTP binding; GTPase activity; protein binding; protein C-terminus binding

Biological Process: activation of MAPKK activity; axon guidance; blood coagulation; cell cycle arrest; cell proliferation; cell surface receptor linked signal transduction; chemotaxis; endocytosis; ephrin receptor signaling pathway; epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; innate immune response; insulin receptor signaling pathway; leukocyte migration; MAPKKK cascade; mitotic cell cycle checkpoint; negative regulation of cell proliferation; negative regulation of neuron apoptosis; nerve growth factor receptor signaling pathway; organ morphogenesis; positive regulation of cell migration; positive regulation of cell proliferation; positive regulation of DNA replication; positive regulation of epithelial cell proliferation; positive regulation of GTPase activity; positive regulation of JNK cascade; positive regulation of MAP kinase activity; positive regulation of MAPKKK cascade; positive regulation of protein amino acid phosphorylation; positive regulation of Ras protein signal transduction; positive regulation of transcription from RNA polymerase II promoter; protein heterooligomerization; Ras protein signal transduction; regulation of long-term neuronal synaptic plasticity; signal transduction; small GTPase mediated signal transduction; social behavior; stimulatory C-type lectin receptor signaling pathway; synaptic transmission; vascular endothelial growth factor receptor signaling pathway

Disease: Bladder Cancer; Costello Syndrome; Melanocytic Nevus Syndrome, Congenital; Nevus, Epidermal; Schimmelpenning-feuerstein-mims Syndrome; Thyroid Carcinoma, Follicular