Mouse IgG Agarose Secondary Antibody

Anti-Mouse IgG Agarose

Cat. Num. AAA330182

• Reactivity: Mouse
• Applications: Immunoprecipitation
• Synonyms: Mouse IgG Agarose; Anti-Mouse IgG Agarose; Anti-Mouse IgG; Agarose; Mouse IgG Agarose secondary antibody

• Host: Goat
• Reactivity: Mouse
• Isotype: IgG
• Form/Format: 1mL beads in 2mL glycerol.

• Applicable Applications for Mouse IgG Agarose secondary antibody: Affinity Chromatography, Immunoprecipitation (IP)
• Ligand Immobilized: 40 mg specific affinity-purified anti-mouse IgG/mL
• Binding capacity: >10 mg/mL of mouse IgG
• Preparation and Storage: One year at -20 degree C from date of shipment. A void cycles of freezing and thawing.

Test Data

(Purification of mouse IgG from monoclonal cell culture supernatant with anti-mouse IgG agarose.Fraction 1: 0.8 mg/mLFraction 2: 2.5 mg/mLIgG Std: 0.31 - 5 mg/mL)

Related Product Information for Mouse IgG Agarose secondary antibody

For Monoclonal antibody production from cell culture or ascites, to avoid the fetal calf serum contamination. Generally the purification of the mouse antibody from the cell culture supernatant using Protein A/G systems may result in contamination with bovine IgG. Anti-mouse IgG agarose is the mouse IgG-specific affinity matrix that could selectively purify mouse IgG from mouse serum, both ascites and the supernatant of the cell culture media, without pulling-down bovine IgG. This anti-mouse JgG agarose is prepared using antigen-specific affinity purified anti-mouse IgG as the immobilized antibodies. More than 20 mg of pure anti-mouse IgG was covalently immobilized onto the agarose beads via a highly stable thiol-ether bond. This product has been successfully used to replace Protein A/Ci agarose for more specific affinity purification of mouse IgG with high binding capacity (> 10 mg/mL).