Western Blot (WB)
(Western Blot (WB) analysis of Raptor (phospho-Ser792) polyclonal antibody at 1:500 dilutionLane1:HEK293T whole cell lysateLane2:HEK293T treated with Etoposide (20ng/ml) for 15 minutes whole cell lysateLane3:HEK293T treated with Etoposide (20ng/ml) for 6 hours whole cell lysate)
Rabbit Raptor Polyclonal Antibody | anti-Ser792 antibody
Raptor (Phospho-Ser792) Polyclonal Antibody
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Synonyms
Raptor; Polyclonal Antibody; Raptor (Phospho-Ser792) Polyclonal Antibody; Regulatory-associated protein of mTOR; p150 target of rapamycin (TOR)-scaffold protein; RPTOR; KIAA1303; RAPTOR; anti-Ser792 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Raptor (phospho-Ser792) polyclonal antibody detects endogenous levels of Raptor protein only when phosphorylated at Ser792.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Applicable Applications for anti-Ser792 antibody
Western Blot (WB)
Application Notes
WB: 1:500-1:1000
Immunogen
Synthetic phosphopeptide derived from human Raptor around the phosphorylation site of Ser792.
Preparation and Storage
Store at 4 degree C short term.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Western Blot (WB)
(Western Blot (WB) analysis of Raptor (phospho-Ser792) polyclonal antibody at 1:500 dilutionLane1:HEK293T whole cell lysateLane2:HEK293T treated with Etoposide (20ng/ml) for 15 minutes whole cell lysateLane3:HEK293T treated with Etoposide (20ng/ml) for 6 hours whole cell lysate)
Western Blot (WB)
(Western Blot (WB) analysis of Raptor (phospho-Ser792) polyclonal antibody at 1:500 dilutionLane1:HEK293T whole cell lysateLane2:HEK293T treated with Etoposide (20ng/ml) for 15 minutes whole cell lysateLane3:HEK293T treated with Etoposide (20ng/ml) for 6 hours whole cell lysate)
Related Product Information for anti-Ser792 antibody
Regulatory associated protein of FRAP, also designated Raptor, is a binding partner for mammalian target of rapamycin kinase (FRAP), and is essential for FRAP signalling in vivo. Raptor binding to FRAP is critical for FRAP-catalysed substrate phosphorylation of 4E-BP1. The raptor-FRAP complex is nutrient-sensitive and is important for a mechanism by which cells coordinate cell growth and size with changing environmental conditions. Raptor serves as a negative regulator of FRAP kinase activity under nutrient-deprived conditions and is an important component in the FRAP pathway. Raptor is highly expressed in skeletal muscle and to a lesser extent in brain, kidney, lung and placenta.
NCBI and Uniprot Product Information
UniProt Accession #
Molecular Weight
~ 150kDa
Similar Products
Product Notes
The Ser792 (Catalog #AAA3000258) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Raptor (Phospho-Ser792) Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Raptor can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:1000. Researchers should empirically determine the suitability of the Ser792 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Raptor, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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