Rabbit anti-Human, Mouse DCXR Polyclonal Antibody | anti-DCXR antibody

DCXR, ID (Sperm Surface Protein P34H, Carbonyl Reductase II, Kidney Dicarbonyl Reductase, kiDCR, Dicarbonyl/L-xylulose Reductase, L-xylulose Reductase, XR) (MaxLight 650)

Cat. Num. AAA6472028

• Gene Names: DCXR; XR; DCR; HCR2; P34H; HCRII; KIDCR; PNTSU; SDR20C1
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: DCXR; Polyclonal Antibody; ID (Sperm Surface Protein P34H; Carbonyl Reductase II; Kidney Dicarbonyl Reductase; kiDCR; Dicarbonyl/L-xylulose Reductase; L-xylulose Reductase; XR) (MaxLight 650); anti-DCXR antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human DCXR. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight650.

• Applicable Applications for anti-DCXR antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:50-1:100; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the center region of human DCXR (KLH).
• Conjugate: MaxLight650
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-DCXR Antibody (Center) in mouse lung tissue lysates (35ug/lane). DCXR (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human kidney carcinoma with DCXR Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of NCI-H292 cells using DCXR Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-DCXR antibody

Antibodies; Enzymes; Reductase

References

1. Nakagawa J., Ishikura S.J. Biol. Chem. 277:17883-17891(2002). 2. Xia X.Y., Xu X.F., Gao Y.Zhonghua Nan Ke Xue 9:24-27(2003). 3. El-Kabbani O., Chung R.P.-T.Acta Crystallogr. D 58:1379-1380(2002).

NCBI and Uniprot Product Information

• NCBI GI #: 7705925
• NCBI GeneID: 51181
• NCBI Accession #: NP_057370
• NCBI GenBank Nucleotide #: NM_016286.3
• UniProt Accession #: Q7Z4W1; Q9BTZ3; Q9UHY9
• Molecular Weight: 28 kDa (264aa), confirmed by MALDI-TOF
• NCBI Official Full Name: L-xylulose reductase isoform 1
• NCBI Official Synonym Full Names: dicarbonyl and L-xylulose reductase
• NCBI Official Symbol: DCXR
• NCBI Official Synonym Symbols: XR; DCR; HCR2; P34H; HCRII; KIDCR; PNTSU; SDR20C1
• NCBI Protein Information: L-xylulose reductase
• UniProt Protein Name: L-xylulose reductase
• UniProt Gene Name: DCXR
• UniProt Synonym Gene Names: XR; kiDCR
• UniProt Entry Name: DCXR_HUMAN

NCBI Description

The protein encoded by this gene acts as a homotetramer to catalyze diacetyl reductase and L-xylulose reductase reactions. The encoded protein may play a role in the uronate cycle of glucose metabolism and in the cellular osmoregulation in the proximal renal tubules. Defects in this gene are a cause of pentosuria. Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Aug 2010]

Uniprot Description

DCXR: Catalyzes the NADPH-dependent reduction of several pentoses, tetroses, trioses, alpha-dicarbonyl compounds and L- xylulose. Participates in the uronate cycle of glucose metabolism. May play a role in the water absorption and cellular osmoregulation in the proximal renal tubules by producing xylitol, an osmolyte, thereby preventing osmolytic stress from occurring in the renal tubules. The enzyme defect in pentosuria has been shown to involve L-xylulose reductase. Essential pentosuria is an inborn error of metabolism characterized by the excessive urinary excretion of the pentose L-xylulose. Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Protein type: Oxidoreductase; Carbohydrate Metabolism - pentose and glucuronate interconversions; EC 1.1.1.10

Chromosomal Location of Human Ortholog: 17q25.3

Cellular Component: microvillus; membrane; cytoplasmic microtubule; nucleus; brush border

Molecular Function: oxidoreductase activity, acting on NADH or NADPH, quinone or similar compound as acceptor; L-xylulose reductase activity

Biological Process: glucose metabolic process; xylulose metabolic process; D-xylose metabolic process; NADP metabolic process; protein homotetramerization

Disease: Pentosuria