Western Blot (WB)
(Western Blot (WB) analysis of RAPH1 polyclonal antibody at 1:500 dilutionLane1:HepG2 whole cell lysateLane2:H9C2 whole cell lysate)
Rabbit anti-Human, Rat RAPH1 Polyclonal Antibody | anti-RAPH1 antibody
RAPH1 Polyclonal Antibody
Reactivity
Human, Rat
Applications
Western Blot
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Synonyms
RAPH1; Polyclonal Antibody; RAPH1 Polyclonal Antibody; Ras-associated and pleckstrin homology domains-containing protein 1; Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 18 protein; Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 9 protein; Lamellipodin; Proline-rich EVH1 ligand 2; PREL-2; Protein RMO1; ALS2CR18; ALS2CR9; KIAA1681; LPD; PREL2; RMO1; anti-RAPH1 antibody
Host
Rabbit
Reactivity
Human, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
RAPH1 polyclonal antibody detects endogenous levels of RAPH1 protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
644
Applicable Applications for anti-RAPH1 antibody
Western Blot (WB)
Application Notes
WB: 1:500-1:1000
Immunogen
A synthetic peptide corresponding to residues in Human RAPH1.
Preparation and Storage
Store at 4 degree C short term.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Western Blot (WB)
(Western Blot (WB) analysis of RAPH1 polyclonal antibody at 1:500 dilutionLane1:HepG2 whole cell lysateLane2:H9C2 whole cell lysate)
Western Blot (WB)
(Western Blot (WB) analysis of RAPH1 polyclonal antibody at 1:500 dilutionLane1:HepG2 whole cell lysateLane2:H9C2 whole cell lysate)
Related Product Information for anti-RAPH1 antibody
Lamellipodin, also called ras-associated and pleckstrin homology domains-containing protein 1 (RAPH1), is a 1302 amino acid member of the MRL family. The peripheral membrane protein mediates localized membrane signals and colocalizes at the tips of filopodia and lamellipodia with ENAH/VASP. Aside from the main isoform, nine additional isoforms have been identified for Lamellipodin (RMO1, RMO1a, RMO1b, RMO1c, RMO1ab, RMO1ac, RMO1bc, RMO1abc and RMO1-RAPH1). RMO1-RAPH1 is expressed in a wide variety of tissues, most highly in brain, heart, ovary and developing embryo. RMO1 is also widely expressed, with highest amounts in liver. Lamellipodin is down regulated in breast and ovarian cancers and shows reduced expression in metastatic osteosarcomas in comparison to primary osteosarcoma tumors.
NCBI and Uniprot Product Information
NCBI GI #
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
~ 135kDa
NCBI Official Full Name
ras-associated and pleckstrin homology domains-containing protein 1 isoform 3
UniProt Protein Name
Ras-associated and pleckstrin homology domains-containing protein 1
UniProt Gene Name
RAPH1
UniProt Synonym Gene Names
ALS2CR18; ALS2CR9; KIAA1681; LPD; PREL2; RMO1; RAPH1; PREL-2
UniProt Entry Name
RAPH1_HUMAN
Uniprot Description
RAPH1: Mediator of localized membrane signals. Implicated in the regulation of lamellipodial dynamics. Negatively regulates cell adhesion. Belongs to the MRL family. 9 isoforms of the human protein are produced by alternative splicing.
Protein type: Motility/polarity/chemotaxis; Adaptor/scaffold
Chromosomal Location of Human Ortholog: 2q33
Cellular Component: cytoskeleton; lamellipodium; cytoplasm; plasma membrane; filopodium
Molecular Function: protein binding
Biological Process: axon extension; cell-matrix adhesion; signal transduction
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Product Notes
The RAPH1 raph1 (Catalog #AAA3006466) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RAPH1 Polyclonal Antibody reacts with Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's RAPH1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:1000. Researchers should empirically determine the suitability of the RAPH1 raph1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RAPH1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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