Immunofluorescence (IF)
(MBS9605835 staining Hela by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Rabbit RAPGEF3 Polyclonal Antibody | anti-RAPGEF3 antibody
RAPGEF3 Antibody
Gene Names
RAPGEF3; EPAC; EPAC1; bcm910; HSU79275; CAMP-GEFI
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunofluorescence, Immunocytochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
RAPGEF3; Polyclonal Antibody; RAPGEF3 Antibody; bcm910; CAMP GEFI; cAMP regulated guanine nucleotide exchange factor I; CAMPGEFI; CGEF 1; CGEF1; EPA1; Epac 1; EPAC; EPAC1; Exchange factor directly activated by cAMP 1; Exchange protein directly activated by cAMP 1; MGC21410; RAP guanine nucleotide exchange factor; Rap guanine nucleotide exchange factor (GEF) 3; RAP guanine nucleotide exchange factor 3; Rap1 guanine nucleotide exchange factor directly activated by cAMP; anti-RAPGEF3 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
RAPGEF3 antibody detects endogenous levels of total RAPGEF3
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
881
Applicable Applications for anti-RAPGEF3 antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes
WB: 1:500-1:1000
IF/ICC: 1:100-1:500
IF/ICC: 1:100-1:500
Immunogen
A synthesized peptide
Subcellular Location
Endomembrane System
Tissue Specificity
Widely expressed with highest levels in adult kidney, heart, thyroid and brain, and fetal kidney.
Predicted Cross Reactivity
Pig, Bovine, Horse, Sheep, Rabbit, Dog
Similarity
Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (86%), Dog (100%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Immunofluorescence (IF)
(MBS9605835 staining Hela by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Immunofluorescence (IF)
(MBS9605835 staining Hela by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Western Blot (WB)
(Western blot analysis of extracts from Jurkat using RAPGEF3 antibody.)
Western Blot (WB)
(Western blot analysis of extracts from Jurkat using RAPGEF3 antibody.)
Related Product Information for anti-RAPGEF3 antibody
Function: Guanine nucleotide exchange factor (GEF) for RAP1A and RAP2A small GTPases that is activated by binding cAMP. Through simultaneous binding of PDE3B to RAPGEF3 and PIK3R6 is assembled in a signaling complex in which it activates the PI3K gamma complex and which is involved in angiogenesis. Plays a role in the modulation of the cAMP-induced dynamic control of endothelial barrier function through a pathway that is independent on Rho-mediated signaling. Required for the actin rearrangement at cell-cell junctions, such as stress fibers and junctional actin.
Subunit Structure: Interacts with PDE3B.
Similarity: The DEP domain is involved in membrane localization independent from regulation by cAMP.
Subunit Structure: Interacts with PDE3B.
Similarity: The DEP domain is involved in membrane localization independent from regulation by cAMP.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 110; 100 kD
Predicted: 104 kDa
Predicted: 104 kDa
NCBI Official Full Name
rap guanine nucleotide exchange factor 3 isoform b
NCBI Official Synonym Full Names
Rap guanine nucleotide exchange factor 3
NCBI Official Symbol
RAPGEF3
NCBI Official Synonym Symbols
EPAC; EPAC1; bcm910; HSU79275; CAMP-GEFI
NCBI Protein Information
rap guanine nucleotide exchange factor 3
UniProt Protein Name
Rap guanine nucleotide exchange factor 3
Protein Family
UniProt Gene Name
RAPGEF3
UniProt Synonym Gene Names
CGEF1; EPAC; EPAC1; EPAC 1; cAMP-GEFI
Uniprot Description
Guanine nucleotide exchange factor (GEF) for RAP1A and RAP2A small GTPases that is activated by binding cAMP. Through simultaneous binding of PDE3B to RAPGEF3 and PIK3R6 is assembled in a signaling complex in which it activates the PI3K gamma complex and which is involved in angiogenesis. Plays a role in the modulation of the cAMP-induced dynamic control of endothelial barrier function through a pathway that is independent on Rho-mediated signaling. Required for the actin rearrangement at cell-cell junctions, such as stress fibers and junctional actin.
Research Articles on RAPGEF3
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Product Notes
The RAPGEF3 rapgef3 (Catalog #AAA9605835) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RAPGEF3 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's RAPGEF3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA). WB: 1:500-1:1000 IF/ICC: 1:100-1:500. Researchers should empirically determine the suitability of the RAPGEF3 rapgef3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RAPGEF3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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