Human ficolin (collagen/fibrinogen domain containing) 3 (Hakata antigen) ELISA Kit | FCN3 elisa kit

Human Ficolin 3, FCN3 ELISA Kit

Cat. Num. AAA701581

• Gene Names: FCN3; FCNH; HAKA1
• Reactivity: Human
• Synonyms: ficolin (collagen/fibrinogen domain containing) 3 (Hakata antigen); Human Ficolin 3; FCN3 ELISA Kit; Human Ficolin 3 (FCN3) ELISA Kit; FCNH; HAKA1; MGC22543; H-ficolin; collagen/fibrinogen domain-containing lectin 3 p35; collagen/fibrinogen domain-containing protein 3; ficolin 3; FCN3 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human FCN3. No significant cross-reactivity or interference between human FCN3 and analogues was observed.
• Sequence Length: 299

• Samples: Serum, plasma, cell culture supernates, tissue homogenates.
• Assay Type: Sandwich
• Detection Range: 7.8 ng/ml -500 ng/ml.
• Sensitivity: 1.95 ng/ml.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FCN3 elisa kit

Principle of the Assay||This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for FCN3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any FCN3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FCN3 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FCN3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 27754776
• NCBI GeneID: 8547
• NCBI Accession #: NP_003656.2
• NCBI GenBank Nucleotide #: NM_003665.2
• UniProt Accession #: O75636; Q6IBJ5; Q8WW86
• Molecular Weight: 32,903 Da
• NCBI Official Full Name: ficolin-3 isoform 1
• NCBI Official Synonym Full Names: ficolin (collagen/fibrinogen domain containing) 3
• NCBI Official Symbol: FCN3
• NCBI Official Synonym Symbols: FCNH; HAKA1
• NCBI Protein Information: ficolin-3; H-ficolin; ficolin 3; collagen/fibrinogen domain-containing protein 3; collagen/fibrinogen domain-containing lectin 3 p35; ficolin (collagen/fibrinogen domain containing) 3 (Hakata antigen)
• UniProt Protein Name: Ficolin-3
• Protein Family: Ficolin
• UniProt Gene Name: FCN3
• UniProt Synonym Gene Names: FCNH; HAKA1
• UniProt Entry Name: FCN3_HUMAN

NCBI Description

Ficolins are a group of proteins which consist of a collagen-like domain and a fibrinogen-like domain. In human serum, there are two types of ficolins, both of which have lectin activity. The protein encoded by this gene is a thermolabile beta-2-macroglycoprotein found in all human serum and is a member of the ficolin/opsonin p35 lectin family. The protein, which was initially identified based on its reactivity with sera from patients with systemic lupus erythematosus, has been shown to have a calcium-independent lectin activity. The protein can activate the complement pathway in association with MASPs and sMAP, thereby aiding in host defense through the activation of the lectin pathway. Alternative splicing occurs at this locus and two variants, each encoding a distinct isoform, have been identified. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: May function in innate immunity through activation of the lectin complement pathway. Calcium-dependent and GlcNAc-binding lectin. Has affinity with GalNAc, GlcNAc, D-fucose, as mono/oligosaccharide and lipopolysaccharides from S.typhimurium and S.minnesota.1 PublicationManual assertion based on experiment in:Ref.9