Rabbit anti-Human, Mouse RAP80 Polyclonal Antibody | anti-UIMC1 antibody

RAP80 Antibody

Cat. Num. AAA150198

• Gene Names: UIMC1; RAP80; X2HRIP110
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: RAP80 Antibody is affinity chromatography purified via peptide column.
• Synonyms: RAP80; Polyclonal Antibody; RAP80 Antibody; X2HRIP110; RXRIP110; BRCA1-A complex subunit RAP80; Receptor-associated protein 80; ubiquitin interaction motif containing 1; anti-UIMC1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: RAP80 Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 719

• Applicable Applications for anti-UIMC1 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: RAP80 antibody can be used for detection of RAP80 by Western blot at 2 mug/mL. Antibody can also be used for immunohistochemistry starting at 2.5 mug/mL. For immunofluorescence start at 20 mug/mL.
• Conjugate: Unconjugated
• Immunogen: RAP80 antibody was raised against a 17 amino acid synthetic peptide from near the carboxy terminus of human RAP80.
• Buffer: RAP80 Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: RAP80 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of RAP80 in 293 cell lysate in (A) the absence and (B) presence of blocking peptide with RAP80 antibody at 2 μg/mL.)

Immunohistochemistry (IHC)

(Immunohistochemistry of RAP80 in human spleen tissue with RAP80 antibody at 2.5 μg/mL.)

Immunofluorescence (IF)

(Immunofluorescence of RAP80 in Human Spleen cells with RAP80 antibody at 20 μg/mL.)

Related Product Information for anti-UIMC1 antibody

RAP80 Antibody: RAP80 was initially identified as zinc-finger containing nuclear protein that is highly expressed in testis and interacts with the retinoid-related testis-associated receptor (RTR). Later experiments revealed that RAP80 is recruited by the Coiled-coil domain 98 (CCDC98) protein to the breast cancer-1 protein BRCA1, allowing the formation of BRCA1 foci in response to DNA damage caused by ionizing radiation. Both RAP80 and CCDC98 are required for DNA damage resistance, G2-M checkpoint control, and DNA repair. Cells depleted of either RAP80 or CCDC98 exhibited increased sensitivity to ionizing radiation, although not as much as in BRCA1-depleted cells, suggesting that RAP80 and CCDC98 control only part of the DNA damage response role of BRCA1. At least four isoforms of RAP80 are known to exist.

NCBI and Uniprot Product Information

• NCBI GI #: 119605449
• NCBI GeneID: 51720
• UniProt Accession #: Q96RL1; Q5XKQ1; Q7Z3W7; Q8N5B9; Q9BZR1; Q9BZR5; Q9UHX7; A8MSA1; B3KMZ1; B4E3N2
• Molecular Weight: 17,412 Da
• NCBI Official Full Name: receptor associated protein 80, isoform CRA_c
• NCBI Official Synonym Full Names: ubiquitin interaction motif containing 1
• NCBI Official Symbol: UIMC1
• NCBI Official Synonym Symbols: RAP80; X2HRIP110
• NCBI Protein Information: BRCA1-A complex subunit RAP80; receptor associated protein 80; receptor-associated protein 80; retinoid x receptor interacting protein; retinoid X receptor-interacting protein 110; ubiquitin interaction motif-containing protein 1
• UniProt Protein Name: BRCA1-A complex subunit RAP80
• Protein Family: BRCA1-A complex
• UniProt Gene Name: UIMC1
• UniProt Synonym Gene Names: RAP80; RXRIP110
• UniProt Entry Name: UIMC1_HUMAN

Uniprot Description

RAP80: Ubiquitin-binding protein that specifically recognizes and binds 'Lys-63'-linked ubiquitin. Plays a central role in the BRCA1-A complex by specifically binding 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). The BRCA1-A complex also possesses deubiquitinase activity that specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX. Also weakly binds monoubiquitin but with much less affinity than 'Lys-63'-linked ubiquitin. May interact with monoubiquitinated histones H2A and H2B; the relevance of such results is however unclear in vivo. Does not bind Lys-48'-linked ubiquitin. May indirectly Act as a transcriptional repressor by inhibiting the interaction of NR6A1 with the corepressor NCOR1. Interacts with TSP57. Component of the BRCA1-A complex, at least composed of the BRCA1, BARD1, UIMC1/RAP80, FAM175A/Abraxas, BRCC3/BRCC36, BRE/BRCC45 and BABAM1/NBA1. In the BRCA1-A complex, interacts directly with FAM175A/Abraxas. Interacts with ESR1, NR6A1 and UBE2I. Expressed in testis, ovary, thymus and heart. Expressed in germ cells of the testis. Belongs to the RAP80 family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Nuclear receptor co-regulator; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 5q35.2

Cellular Component: nucleus

Molecular Function: retinoid X receptor binding; protein binding; histone binding

Biological Process: positive regulation of DNA repair; transcription, DNA-dependent; double-strand break repair; response to ionizing radiation; negative regulation of transcription, DNA-dependent; G2/M transition DNA damage checkpoint