Rabbit anti-Human, Mouse RAP2C Polyclonal Antibody | anti-RAP2C antibody

RAP2C, NT (RAP2C, Ras-related protein Rap-2c) (AP)

Cat. Num. AAA6340284

• Reactivity: Human, Mouse
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: RAP2C; Polyclonal Antibody; NT (RAP2C; Ras-related protein Rap-2c) (AP); Ras-related protein Rap-2c; anti-RAP2C antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-RAP2C antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: RAP2C antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 14-42 amino acids from the N-terminal region of human RAP2C.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(RAP2C Antibody (N-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the RAP2C antibody detected the RAP2C protein (arrow).)

Western Blot (WB)

(RAP2C Antibody (N-term) western blot analysis in mouse stomach tissue lysates (35ug/lane).This demonstrates the RAP2C antibody detected the RAP2C protein (arrow).)

Related Product Information for anti-RAP2C antibody

Small GTP-binding protein which cycles between a GDP-bound inactive and a GTP-bound active form. May play a role in cytoskeletal rearrangements and regulate cell spreading through activation of the effector TNIK. May play a role in SRE-mediated gene transcription.

Product Categories/Family for anti-RAP2C antibody

Antibodies; GTPase; Rab; Ras; Rho Proteins

References

Guo, Z., et al. Mol. Biol. Rep. 34(3):137-144(2007). Paganini, S., et al. Biochimie 88 (3-4), 285-295 (2006): Ross, M.T., et al. Nature 434(7031):325-337(2005).

NCBI and Uniprot Product Information

• NCBI GI #: 32129209
• NCBI GeneID: 57826
• NCBI Accession #: NP_067006.3
• NCBI GenBank Nucleotide #: NM_021183.4
• UniProt Accession #: Q9Y3L5; Q5H9H9; Q9BTS0; B3KWD6
• Molecular Weight: 20,745 Da
• NCBI Official Full Name: ras-related protein Rap-2c isoform 1
• NCBI Official Synonym Full Names: RAP2C, member of RAS oncogene family
• NCBI Official Symbol: RAP2C
• NCBI Protein Information: ras-related protein Rap-2c
• UniProt Protein Name: Ras-related protein Rap-2c
• Protein Family: Ras-related protein
• UniProt Gene Name: RAP2C
• UniProt Entry Name: RAP2C_HUMAN

NCBI Description

The protein encoded by this gene is a member of the Ras-related protein subfamily of the Ras GTPase superfamily. Members of this family are small GTPases that act as molecular switches to regulate cellular proliferation, differentiation, and apoptosis. This protein has been reported to activate in vitro transcriptional activity of the serum response element. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2012]

Uniprot Description

RAP2C: Small GTP-binding protein which cycles between a GDP- bound inactive and a GTP-bound active form. May play a role in cytoskeletal rearrangements and regulate cell spreading through activation of the effector TNIK. May play a role in SRE-mediated gene transcription. Belongs to the small GTPase superfamily. Ras family.

Protein type: G protein, monomeric, Ras; G protein, monomeric; G protein

Chromosomal Location of Human Ortholog: Xq25

Cellular Component: tight junction; recycling endosome membrane; cytoplasm; plasma membrane; cytosol

Molecular Function: GDP binding; GTP binding; transcription coactivator activity

Biological Process: microvillus biogenesis; negative regulation of cell migration; positive regulation of protein amino acid autophosphorylation; Rap protein signal transduction