Testing Data
Mouse anti-Human CD63 Monoclonal Antibody | anti-CD63 antibody
Anti-human CD63 antibody
Reactivity
Human
Applications
ELISA, Flow Cytometry, Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
>95% purified from tissue culture supernatant or ascites by Protein A affinity chromatography
Synonyms
CD63; Monoclonal Antibody; Anti-human CD63 antibody; LAMP-3 (lysosome-associated membrane protein-3); anti-CD63 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
Mouse IgG1
Purity/Purification
>95% purified from tissue culture supernatant or ascites by Protein A affinity chromatography
Form/Format
Liquid
1 mg/ml in phosphate buffered saline (PBS) with sodium azide (15 mM); Approx. pH: 7.4.
1 mg/ml in phosphate buffered saline (PBS) with sodium azide (15 mM); Approx. pH: 7.4.
Concentration
1 mg/ml (varies by lot)
Applicable Applications for anti-CD63 antibody
Western Blot (WB) (using non-reducing conditions); ELISA (EIA); Flow Cytometry (FC); Immunoprecipitation (IP); Immunohistochemistry (IHC)
Application Notes
WB: 1:1000
ELISA: 1:500
FC: 1:200
ELISA: 1:500
FC: 1:200
Immunogen
T-cell line HRB-ALL
Preparation and Storage
Store undiluted at 4 degree C.
DO NOT FREEZE!
Do not use after expiration date.
DO NOT FREEZE!
Do not use after expiration date.
Testing Data
Testing Data
Related Product Information for anti-CD63 antibody
Mouse monoclonal unconjugated antibody to detect CD63 in human samples.
Anti-CD63 recognizes an extracellular fragment of CD63; a 56-kilodalton (kDa); type III lysosomal glycoprotein; belonging to the tetraspanin family. CD63 is expressed by granulocytes; platelets; T-cells; monocytes/macrophages; endothelial cells. CD63 protein is also expressed in exosome membrane. Cell sur-face exposition of CD63 is usually activation dependent. CD63 interacts with integrins and affects phagocytosis and cell migration; it is also involved in H/K ATPase trafficking regulation of ROMK1 channels. CD63 also serves as a T-cell co-stimulator molecule. Expression of CD63 can be used for predicting the prognosis in earlier stages of carcinomas.
Anti-CD63 recognizes an extracellular fragment of CD63; a 56-kilodalton (kDa); type III lysosomal glycoprotein; belonging to the tetraspanin family. CD63 is expressed by granulocytes; platelets; T-cells; monocytes/macrophages; endothelial cells. CD63 protein is also expressed in exosome membrane. Cell sur-face exposition of CD63 is usually activation dependent. CD63 interacts with integrins and affects phagocytosis and cell migration; it is also involved in H/K ATPase trafficking regulation of ROMK1 channels. CD63 also serves as a T-cell co-stimulator molecule. Expression of CD63 can be used for predicting the prognosis in earlier stages of carcinomas.
Product Categories/Family for anti-CD63 antibody
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Product Notes
The CD63 (Catalog #AAA846149) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-human CD63 antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD63 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB) (using non-reducing conditions); ELISA (EIA); Flow Cytometry (FC); Immunoprecipitation (IP); Immunohistochemistry (IHC). WB: 1:1000 ELISA: 1:500 FC: 1:200. Researchers should empirically determine the suitability of the CD63 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD63, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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