Rabbit RAP2B Polyclonal Antibody | anti-RAP2B antibody

RAP2B Polyclonal Antibody

Cat. Num. AAA3007541

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: RAP2B; Polyclonal Antibody; RAP2B Polyclonal Antibody; Ras-related protein Rap-2b; anti-RAP2B antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: RAP2B polyclonal antibody detects endogenous levels of RAP2B protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 183

• Applicable Applications for anti-RAP2B antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: Synthetic peptide, corresponding Human RAP2B.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of RAP2B polyclonal antibody at 1:500 dilutionLane1:K562 whole cell lysate (40ug) Lane2:Jurkat whole cell lysate (40ug) Lane3:THP-1 whole cell lysate (40ug) Lane4:BV2 whole cell lysate (40ug) Lane5:PMVEC whole cell lysate (40ug))

Related Product Information for anti-RAP2B antibody

Ras oncogenes encode GTP-binding proteins that are capable of transforming immortalized cells in culture. Two Ras-related human genes, designated RAP1A and RAP1B, encode 95% homologous proteins (namely Rap 1A and Rap 1B) that share a similar C-terminal Cys-Ali-Ali-Xaa sequence with Ras proteins and are ubiquitously expressed in mammalian tissues. The putative "effector" domain of Ras proteins, whose integrity is required for cell transformation as well as interaction with the putative effector protein GAP, is conserved in both Rap 1 proteins. Rap 1A is thought to interfere with Ras effector function by binding to Ras GAP in a GTP-dependent manner without affecting Rap 1A GTPase activity. Rap 2, another Ras-related protein, shares 60% identity with Rap 1A and exhibits a carboxy-terminal CAAX motif and two upstream cysteines similar to those of the H-Ras, K-Ras and N-Ras proteins. In contrast with Rap 1A and Rap 1B, overexpression of Rap 2 does not interfere with the Ras signaling pathway.

NCBI and Uniprot Product Information

• NCBI GI #: 38201690
• NCBI GeneID: 5912
• NCBI Accession #: NP_002877.2
• NCBI GenBank Nucleotide #: NP_002877.2
• UniProt Accession #: P61225
• Molecular Weight: ~ 21kDa
• NCBI Official Full Name: ras-related protein Rap-2b
• NCBI Official Synonym Full Names: RAP2B, member of RAS oncogene family
• NCBI Official Symbol: RAP2B
• NCBI Protein Information: ras-related protein Rap-2b
• UniProt Protein Name: Ras-related protein Rap-2b
• Protein Family: Ras-related protein
• UniProt Gene Name: RAP2B
• UniProt Entry Name: RAP2B_HUMAN

NCBI Description

This intronless gene belongs to a family of RAS-related genes. The proteins encoded by these genes share approximately 50% amino acid identity with the classical RAS proteins and have numerous structural features in common. The most striking difference between the RAP and RAS proteins resides in their 61st amino acid: glutamine in RAS is replaced by threonine in RAP proteins. Evidence suggests that this protein may be polyisoprenylated and palmitoylated. [provided by RefSeq, Jul 2008]

Uniprot Description

RAP2B: Small GTP-binding protein which cycles between a GDP- bound inactive and a GTP-bound active form. Involved in EGFR and CHRM3 signaling pathways through stimulation of PLCE1. May play a role in cytoskeletal rearrangements and regulate cell spreading through activation of the effector TNIK. May regulate membrane vesiculation in red blood cells. Belongs to the small GTPase superfamily. Ras family.

Protein type: G protein, monomeric, Ras; G protein, monomeric; G protein

Chromosomal Location of Human Ortholog: 3q25.2

Cellular Component: tight junction; membrane; recycling endosome membrane; plasma membrane; cytosol; lipid raft

Molecular Function: protein domain specific binding; GDP binding; GTP binding

Biological Process: microvillus biogenesis; platelet activation; signal transduction; positive regulation of protein amino acid autophosphorylation; negative regulation of cell migration; Rap protein signal transduction