Rabbit Rho G Polyclonal Antibody | anti-RhoG antibody

Rho G (Q132) Polyclonal Antibody

Cat. Num. AAA3004459

• Gene Names: RHOG; ARHG
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: Rho G; Polyclonal Antibody; Rho G (Q132) Polyclonal Antibody; ARHG; Ras homolog family member G; Rho related GTP binding protein RhoG precursor; Rho-related GTP-binding protein RhoG; RHOG; anti-RhoG antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Rho G (Q132) polyclonal antibody detects endogenous levels of Rho G protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 191

• Applicable Applications for anti-RhoG antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 100-150 of Human Rho G.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of Rho G (Q132) pAb at 1:500 dilutionLane1:The Spleen tissue lysate of Mouse (40ug) Lane2:The Spleen tissue lysate of Rat (40ug) Lane3:K562 whole cell lysate (40ug) Lane4:H1792 whole cell lysate (40ug))

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of Rho G (Q132) pAb in paraffin-embedded human colorectal carcinoma tissue at 1:50.)

Related Product Information for anti-RhoG antibody

The Ras p21 family of guanine nucleotide proteins has been widely studied in view of its apparent role in signal transduction pathways and high frequency of mutations in human malignancies. It is now clear, however, that the Ras proteins (H-, K- and N-Ras p21) are members of a much larger superfamily of related proteins. Six members of this family, Rap 1A, Rap 1B, Rap 2, R-Ras, Ral A and Ral B, exhibit approximately 50% amino acid homology to Ras. The six mammalian Rho proteins (Rho A, B, C, G, 7 and 8) are approximately 30% homologous to Ras and are expressed in a wide range of cell types. Both Ras p21 and Rho p21, as well as other members of the Ras superfamily, contain a carboxy-terminal CAAX sequence (C, cysteine; A, aliphatic amino acid; X, any amino acid) which in the case of Ras has been shown to be essential for correct localization and function.

NCBI and Uniprot Product Information

• NCBI GI #: 46249393
• NCBI GeneID: 391
• NCBI Accession #: NP_001656.2
• NCBI GenBank Nucleotide #: NP_001656.2
• UniProt Accession #: P84095
• Molecular Weight: ~ 21kDa
• NCBI Official Full Name: rho-related GTP-binding protein RhoG
• NCBI Official Synonym Full Names: ras homolog family member G
• NCBI Official Symbol: RHOG
• NCBI Official Synonym Symbols: ARHG
• NCBI Protein Information: rho-related GTP-binding protein RhoG
• UniProt Protein Name: Rho-related GTP-binding protein RhoG
• Protein Family: Rho GTPase-activating protein
• UniProt Gene Name: RHOG
• UniProt Synonym Gene Names: ARHG
• UniProt Entry Name: RHOG_HUMAN

NCBI Description

This gene encodes a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades. Rho proteins promote reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility. The encoded protein facilitates translocation of a functional guanine nucleotide exchange factor (GEF) complex from the cytoplasm to the plasma membrane where ras-related C3 botulinum toxin substrate 1 is activated to promote lamellipodium formation and cell migration. Two related pseudogene have been identified on chromosomes 20 and X. [provided by RefSeq, Aug 2011]

Uniprot Description

RHOG: Required for the formation of membrane ruffles during macropinocytosis. Plays a role in cell migration and is required for the formation of cup-like structures during trans-endothelial migration of leukocytes. In case of Salmonella enterica infection, activated by SopB and ARHGEF26/SGEF, which induces cytoskeleton rearrangements and promotes bacterial entry. Belongs to the small GTPase superfamily. Rho family.

Protein type: G protein, monomeric; Motility/polarity/chemotaxis; G protein, monomeric, Rho

Chromosomal Location of Human Ortholog: 11p15.5-p15.4

Cellular Component: focal adhesion; plasma membrane; cytosol

Molecular Function: GTPase activity; protein binding; GTP binding

Biological Process: axon guidance; platelet activation; regulation of small GTPase mediated signal transduction; metabolic process; small GTPase mediated signal transduction; positive regulation of transcription, DNA-dependent; positive regulation of cell proliferation; Rac protein signal transduction; actin cytoskeleton organization and biogenesis; blood coagulation; Rho protein signal transduction