Western Blot (WB)
(Western blot analysis of LRG1 in HCT-116 cell lysate in the 1) absence and 2) presence of immunizing peptide and 3) mouse colon lysate using MBS649336 at 0.5ug/ml, 0.5ug/ml and 2ug/ml.)
Rabbit anti-Human, Mouse Leucine-rich alpha-2-glycoprotein Polyclonal Antibody | anti-LRG1 antibody
Leucine-rich alpha-2-glycoprotein (LRG, HMFT1766, Leucine-rich alpha-2-glycoprotein 1, LRG1)
Gene Names
LRG1; LRG; HMFT1766
Reactivity
Human, Mouse
Applications
Western Blot
Purity
Affinity PurifiedPurified by Protein A affinity chromatography.
Synonyms
Leucine-rich alpha-2-glycoprotein; Polyclonal Antibody; Leucine-rich alpha-2-glycoprotein (LRG; HMFT1766; Leucine-rich alpha-2-glycoprotein 1; LRG1); Anti -Leucine-rich alpha-2-glycoprotein (LRG; anti-LRG1 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human LRG1. Species Crossreactivity
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.
Applicable Applications for anti-LRG1 antibody
Western Blot (WB)
Application Notes
Suitable for use in Western Blot.
Dilution: Western Blot: 2-4ug/ml
Dilution: Western Blot: 2-4ug/ml
Immunogen
Synthetic peptide corresponding to aa300-350 of human LRG1.
Positive Control
HCT-116, colon
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western blot analysis of LRG1 in HCT-116 cell lysate in the 1) absence and 2) presence of immunizing peptide and 3) mouse colon lysate using MBS649336 at 0.5ug/ml, 0.5ug/ml and 2ug/ml.)
Western Blot (WB)
(Western blot analysis of LRG1 in HCT-116 cell lysate in the 1) absence and 2) presence of immunizing peptide and 3) mouse colon lysate using MBS649336 at 0.5ug/ml, 0.5ug/ml and 2ug/ml.)
Related Product Information for anti-LRG1 antibody
LRG1 is a 347aa containing leucine-rich secreted protein, belonging to leucine-rich repeat (LRR) family of proteins and contains eight LRR (leucine rich) repeats, a galactosamine and four glucosamine oligosaccharides attached and has two intrachain disulfide bonds. LRG1, a regulatory protein, is usually found in plasma and is expressed during granulocyte differentiation. Different functions of LRG1 include protein-protein interaction, signal transduction, cell adhesion and development. LRG1 expression is detected in neutrophil fraction of bone marrow and peripheral blood and in the bone marrow mononuclear cell fraction. LRG1 expression is usually upregulated in early DMSO-induced granulocyte differentiation of human promyelocytic leukemia HL-60 cells and also in GCSF-treated human cells derived from a patient with myeloproliferative disorder. Thus LRG1 acts as a novel marker for early neutrophilic granulocyte differentiation.
Product Categories/Family for anti-LRG1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
38,178 Da
NCBI Official Full Name
leucine-rich alpha-2-glycoprotein
NCBI Official Synonym Full Names
leucine-rich alpha-2-glycoprotein 1
NCBI Official Symbol
LRG1
NCBI Official Synonym Symbols
LRG; HMFT1766
NCBI Protein Information
leucine-rich alpha-2-glycoprotein; 1300008B03Rik; 2310031E04Rik; leucine rich alpha 2 glycoprotein
UniProt Protein Name
Leucine-rich alpha-2-glycoprotein
Protein Family
UniProt Gene Name
LRG1
UniProt Synonym Gene Names
LRG; LRG
UniProt Entry Name
A2GL_HUMAN
NCBI Description
The leucine-rich repeat (LRR) family of proteins, including LRG1, have been shown to be involved in protein-protein interaction, signal transduction, and cell adhesion and development. LRG1 is expressed during granulocyte differentiation (O'Donnell et al., 2002 [PubMed 12223515]).[supplied by OMIM, Mar 2008]
Uniprot Description
Subcellular location: Secreted.
Tissue specificity: Plasma.
Sequence similarities: Contains 8 LRR (leucine-rich) repeats.Contains 1 LRRCT domain.
Research Articles on LRG1
Similar Products
Product Notes
The LRG1 lrg1 (Catalog #AAA649336) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Leucine-rich alpha-2-glycoprotein (LRG, HMFT1766, Leucine-rich alpha-2-glycoprotein 1, LRG1) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Leucine-rich alpha-2-glycoprotein can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Suitable for use in Western Blot. Dilution: Western Blot: 2-4ug/ml. Researchers should empirically determine the suitability of the LRG1 lrg1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Leucine-rich alpha-2-glycoprotein, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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