Rabbit anti-Human, Mouse RANBP9 Polyclonal Antibody | anti-RANBP9 antibody

RANBP9 Antibody

Cat. Num. AAA9629900

• Gene Names: RANBP9; BPM-L; BPM90; RANBPM; RanBP7
• Reactivity: Human, Mouse
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: RANBP9; Polyclonal Antibody; RANBP9 Antibody; B cell antigen receptor Ig beta associated protein 1; BPM 90; BPM L; BPM-L; BPM90; BPML; IBAP 1; Imp 9; Importin 9; Novel centrosomal protein RanBPM; RAN binding protein 9; Ran binding protein centrosomal; Ran Binding Protein in the Microtubule organizing center; Ran binding protein M; Ran BP9; Ran-binding protein 9; Ran-binding protein M; RANB9_HUMAN; RanBP 7; RANBP 9; RanBP7; RanBP9; RanBPM; anti-RANBP9 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: RANBP9 Antibody detects endogenous levels of RANBP9.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-RANBP9 antibody: ELISA (EIA)(peptide)
• Application Notes: ELISA(peptide): 1:20000-1:40000
• Immunogen: A synthesized peptide derived from human RANBP9.
• Conjugation: Unconjugated
• Similarity: The SPRY domain mediates the interaction with MET, AR, and CDC2L1. Belongs to the RANBP9/10 family.
• Post Translational Modifications: Phosphorylated in response to stress. Can be phosphorylated by the cleaved p110 form of CDC2L1 (p110C).Ubiquitinated. Polyubiquitination targets the protein for rapid degradation via the ubiquitin system. Can be deubiquitinated by USP11.
• Subunit Structure: Part of a complex consisting of RANBP9, MKLN1 and GID8. Identified in the CTLH complex that contains GID4, RANBP9 and/or RANBP10, MKLN1, MAEA, RMND5A (or alternatively its paralog RMND5B), GID8, ARMC8, WDR26 and YPEL5. Within this complex, MAEA, RMND5A (or alternatively its paralog RMND5B), GID8, WDR26, and RANBP9 and/or RANBP10 form the catalytic core, while GID4, MKLN1, ARMC8 and YPEL5 have ancillary roles. Interacts with GTP-bound Ran, AR, CDC2L1/p110C, CALB1, S100A7, USP11, MKLN1, SOS1 or SOS2, GID8, and FMR1. Interacts with the Dyrk kinases HIPK2, DYRK1A, and DYRK1B. Interacts with TP73 isoform Alpha but not with TP53. Interacts with the HGF receptor MET and the integrins ITGB1 and ITGB2, but not with ITGAL. Part of a complex consisting of RANBP9, RAN, DYRK1B and COPS5. Directly interacts with RANBP10. Interacts with YPEL5. Interacts with DDX4. Interacts with NGFR (By similarity). Interacts with TEX19 (By similarity).
• Tissue Specificity: Ubiquitously expressed, with highest levels in testes, placenta, heart, and muscle, and lowest levels in lung. Within the brain, expressed predominantly by neurons in the gray matter of cortex, the granular layer of cerebellum and the Purkinje cells.
• Function: May act as scaffolding protein, and as adapter protein to couple membrane receptors to intracellular signaling pathways (Probable). Acts as a mediator of cell spreading and actin cytoskeleton rearrangement. Core component of the CTLH E3 ubiquitin-protein ligase complex that selectively accepts ubiquitin from UBE2H and mediates ubiquitination and subsequent proteasomal degradation of the transcription factor HBP1. May be involved in signaling of ITGB2/LFA-1 and other integrins. Enhances HGF-MET signaling by recruiting Sos and activating the Ras pathway. Enhances dihydrotestosterone-induced transactivation activity of AR, as well as dexamethasone-induced transactivation activity of NR3C1, but not affect estrogen-induced transactivation. Stabilizes TP73 isoform Alpha, probably by inhibiting its ubiquitination, and increases its proapoptotic activity. Inhibits the kinase activity of DYRK1A and DYRK1B. Inhibits FMR1 binding to RNA.
• Note: The unphosphorylated form is predominantly cytoplasmic. A phosphorylated form is associated with the plasma membrane.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Product Categories/Family for anti-RANBP9 antibody

Cytoplasm; Nucleus; Cell membrane; Peripheral membrane protein; Primary

NCBI and Uniprot Product Information

• NCBI GI #: 61215334
• NCBI GeneID: 10048
• UniProt Accession #: Q96S59; O94764; Q6P3T7; Q7LBR2; Q7Z7F9; A0PJA2; B2R8E1
• Molecular Weight: 729
• NCBI Official Full Name: Ran-binding protein 9
• NCBI Official Synonym Full Names: RAN binding protein 9
• NCBI Official Symbol: RANBP9
• NCBI Official Synonym Symbols: BPM-L; BPM90; RANBPM; RanBP7
• NCBI Protein Information: ran-binding protein 9; ran-binding protein M; novel centrosomal protein RanBPM; ran binding protein, centrosomal; Ran Binding Protein in the Microtubule organizing center
• UniProt Protein Name: Ran-binding protein 9
• Protein Family: Ran-binding protein
• UniProt Gene Name: RANBP9
• UniProt Synonym Gene Names: RANBPM; RanBP9; RanBPM
• UniProt Entry Name: RANB9_HUMAN

NCBI Description

This gene encodes a protein that binds RAN, a small GTP binding protein belonging to the RAS superfamily that is essential for the translocation of RNA and proteins through the nuclear pore complex. The protein encoded by this gene has also been shown to interact with several other proteins, including met proto-oncogene, homeodomain interacting protein kinase 2, androgen receptor, and cyclin-dependent kinase 11. [provided by RefSeq, Jul 2008]

Uniprot Description

RANBP9: May act as an adapter protein to couple membrane receptors to intracellular signaling pathways. May be involved in signaling of ITGB2/LFA-1 and other integrins. Enhances HGF-MET signaling by recruiting Sos and activating the Ras pathway. Enhances dihydrotestosterone-induced transactivation activity of AR, as well as dexamethasone-induced transactivation activity of NR3C1, but not affect estrogen-induced transactivation. Stabilizes TP73 isoform Alpha, probably by inhibiting its ubiquitination, and increases its proapoptotic activity. Inhibits the kinase activity of DYRK1A and DYRK1B. Inhibits FMR1 binding to RNA. Belongs to the RANBP9/10 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Nuclear receptor co-regulator; Adaptor/scaffold; GAPs; GAPs, Ras

Chromosomal Location of Human Ortholog: 6p23

Cellular Component: microtubule associated complex; nucleus; cytosol

Molecular Function: protein binding; enzyme binding; Ran GTPase binding

Biological Process: axon guidance; protein complex assembly; microtubule nucleation