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Western Blot (WB) (Western blot analysis of RANBP9 Antibody (Center) in MDA-MB231 cell line lysates (35ug/lane). RANBP9 (arrow) was detected using the purified Pab.)

Rabbit anti-Human RANBP9 Polyclonal Antibody | anti-RANBP9 antibody

RANBP9, ID (Ran-binding Protein 9, RanBP9, RanBP7, Ran-binding Protein M, RanBPM, BPM90, BPM-L) (MaxLight 490)

Gene Names
RANBP9; BPM-L; BPM90; RANBPM; RanBP7
Reactivity
Human
Applications
Western Blot
Purity
Purified by Ammonium Sulfate Precipitation.
Synonyms
RANBP9; Polyclonal Antibody; ID (Ran-binding Protein 9; RanBP9; RanBP7; Ran-binding Protein M; RanBPM; BPM90; BPM-L) (MaxLight 490); anti-RANBP9 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human RANBP9.
Purity/Purification
Purified by Ammonium Sulfate Precipitation.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight490.
Applicable Applications for anti-RANBP9 antibody
Western Blot (WB), FLISA
Application Notes
WB: 1:50-1:100
FLISA: 1:1,000
Applications are based on unconjugated antibody.
Immunogen
Synthetic peptide selected from the center region of human RANBP9 (KLH).
Conjugate
MaxLight490
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of RANBP9 Antibody (Center) in MDA-MB231 cell line lysates (35ug/lane). RANBP9 (arrow) was detected using the purified Pab.)

Western Blot (WB) (Western blot analysis of RANBP9 Antibody (Center) in MDA-MB231 cell line lysates (35ug/lane). RANBP9 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human skeletal muscle reacted with RANBP9 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded human skeletal muscle reacted with RANBP9 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of MDA-231 cells using RANBP9 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Flow Cytometry (FC/FACS) (Flow cytometric analysis of MDA-231 cells using RANBP9 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Product Categories/Family for anti-RANBP9 antibody
References
1. Caballero, O.L., et.al., Oncogene 21(19), 3003-3010 (2002).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
55,082 Da
NCBI Official Full Name
ran-binding protein 9
NCBI Official Synonym Full Names
RAN binding protein 9
NCBI Official Symbol
RANBP9
NCBI Official Synonym Symbols
BPM-L; BPM90; RANBPM; RanBP7
NCBI Protein Information
ran-binding protein 9; Ran Binding Protein in the Microtubule organizing center; novel centrosomal protein RanBPM; ran binding protein, centrosomal; ran-binding protein M
UniProt Protein Name
Ran-binding protein 9
Protein Family
UniProt Gene Name
RANBP9
UniProt Synonym Gene Names
RANBPM; RanBP9; RanBPM
UniProt Entry Name
RANB9_HUMAN

Uniprot Description

RANBP9: May act as an adapter protein to couple membrane receptors to intracellular signaling pathways. May be involved in signaling of ITGB2/LFA-1 and other integrins. Enhances HGF-MET signaling by recruiting Sos and activating the Ras pathway. Enhances dihydrotestosterone-induced transactivation activity of AR, as well as dexamethasone-induced transactivation activity of NR3C1, but not affect estrogen-induced transactivation. Stabilizes TP73 isoform Alpha, probably by inhibiting its ubiquitination, and increases its proapoptotic activity. Inhibits the kinase activity of DYRK1A and DYRK1B. Inhibits FMR1 binding to RNA. Belongs to the RANBP9/10 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Nuclear receptor co-regulator; Adaptor/scaffold; GAPs; GAPs, Ras

Chromosomal Location of Human Ortholog: 6p23

Cellular Component: microtubule associated complex; nucleus; cytosol

Molecular Function: protein binding; enzyme binding; Ran GTPase binding

Biological Process: axon guidance; protein complex assembly; microtubule nucleation

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Product Notes

The RANBP9 ranbp9 (Catalog #AAA6496788) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RANBP9, ID (Ran-binding Protein 9, RanBP9, RanBP7, Ran-binding Protein M, RanBPM, BPM90, BPM-L) (MaxLight 490) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's RANBP9 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), FLISA. WB: 1:50-1:100 FLISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the RANBP9 ranbp9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RANBP9, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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