Goat RANBP1 Polyclonal Antibody | anti-RANBP1 antibody

Anti-RANBP1 Antibody (aa186-200)

Cat. Num. AAA2400831

• Gene Names: RANBP1; HTF9A
• Reactivity: Human; Predicted: Monkey
• Applications: ELISA, Immunohistochemistry, Western Blot
• Purity: Immunoaffinity Purified
• Synonyms: RANBP1; Polyclonal Antibody; Anti-RANBP1 Antibody (aa186-200); Goat Polyclonal to Human RANBP1; RAN binding protein 1; Ran-binding protein 1; HTF9A; anti-RANBP1 antibody

• Host: Goat
• Reactivity: Human; Predicted: Monkey
• Clonality: Polyclonal
• Specificity: Human RANBP1.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
• Sequence Length: 201

• Applicable Applications for anti-RANBP1 antibody: ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
• Application Notes: ELISA: 1:16000; IHC-P: 3.75 ug/ml; WB: 0.1-1 ug/ml
• Immunogen: Synthetic peptide C-LSVKEETKEDAEEKQ from the C-terminus of human RANBP1 (NP_002873.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%); Marmoset (87%).
• Conjugation: Unconjugated
• Epitope: aa186-200
• Preparation and Storage: Store at -20 degree C.; Avoid freeze-thaw cycles.

Immunohistochemistry (IHC)

(Anti-RANBP1 antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Western Blot (WB)

(Staining (0.1 ug/ml) of HepG2 lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.)

Related Product Information for anti-RANBP1 antibody

Ran/TC4-binding protein , RanBP1, interacts specifically withGTP-charged RAN. RANBP1 encodes a 23-kD protein that binds to RANcomplexed with GTP but not GDP. RANBP1 does not activate GTPase activityof RAN but does markedly increase GTP hydrolysis by the RanGTPase-activating protein (RanGAP1). the RANBP1 cDNA encodes a201-amino acid protein that is 92% similar to its mouse homolog.

NCBI and Uniprot Product Information

• NCBI GI #: 4506407
• NCBI GeneID: 5902
• NCBI Accession #: NP_002873.1
• NCBI GenBank Nucleotide #: NM_002882.3
• UniProt Accession #: P43487; Q53EY3
• Molecular Weight: 23,239 Da
• NCBI Official Full Name: ran-specific GTPase-activating protein isoform 2
• NCBI Official Synonym Full Names: RAN binding protein 1
• NCBI Official Symbol: RANBP1
• NCBI Official Synonym Symbols: HTF9A
• NCBI Protein Information: ran-specific GTPase-activating protein
• UniProt Protein Name: Ran-specific GTPase-activating protein
• Protein Family: Ran-specific GTPase-activating protein
• UniProt Gene Name: RANBP1
• UniProt Synonym Gene Names: RanBP1

NCBI Description

This gene encodes a protein that forms a complex with Ras-related nuclear protein (Ran) and metabolizes guanoside triphosphate (GTP). This complex participates in the regulation of the cell cycle by controlling transport of proteins and nucleic acids into the nucleus. There are multiple pseudogenes for this gene on chromosomes 9, 12, 17, and X. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]

Uniprot Description

Plays a role in RAN-dependent nucleocytoplasmic transport. Alleviates the TNPO1-dependent inhibition of RAN GTPase activity and mediates the dissociation of RAN from proteins involved in transport into the nucleus (). Induces a conformation change in the complex formed by XPO1 and RAN that triggers the release of the nuclear export signal of cargo proteins (PubMed:20485264). Promotes the disassembly of the complex formed by RAN and importin beta. Promotes dissociation of RAN from a complex with KPNA2 and CSE1L (). Required for normal mitotic spindle assembly and normal progress through mitosis via its effect on RAN (PubMed:17671426). Does not increase the RAN GTPase activity by itself, but increases GTP hydrolysis mediated by RANGAP1 (PubMed:7882974). Inhibits RCC1-dependent exchange of RAN-bound GDP by GTP (PubMed:7882974, PubMed:7616957).