Goat RANBP1 Polyclonal Antibody | anti-RANBP1 antibody

Goat anti-RANBP1 Antibody

Cat. Num. AAA420729

• Gene Names: RANBP1; HTF9A
• Reactivity: Tested: Human; Expected from sequence similarity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: RANBP1; Polyclonal Antibody; Goat anti-RANBP1 Antibody; RAN binding protein 1; HTF9A; MGC88701; RANBP1 antibody; RAN binding protein 1 antibody; anti-RANBP1 antibody

• Host: Goat
• Reactivity: Tested: Human; Expected from sequence similarity: Human
• Clonality: Polyclonal
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Concentration: 100ug specific antibody in 200ul (varies by lot)
• Sequence: LSVKEETKEDAEEKQ
• Sequence Length: 201

• Applicable Applications for anti-RANBP1 antibody: Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Peptide ELISA: Antibody detection limit dilution 1: 16000.; Immunohistochemistry: In paraffin embedded Human Kidney shows pixulated cytoplasm staining in DCT. Recommended concentration, 3-5ug/ml.; Western Blot: Approx 26kDa band observed in lysate of cell line HeLa (calculated MW of 23.3kDa according to NP_002873.1). Recommended concentration: 0.05-0.1ug/ml.
• Immunogen: Peptide with sequence C-LSVKEETKEDAEEKQ, from the C Terminus of the protein sequence according to NP_002873.1.
• Epitope: C Terminus
• Preparation and Storage: Aliquot and store at -20 degree C. Minimize freezing and thawing.

Western Blot (WB)

((0. 05ug/ml) staining of HELA lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. chemiluminescence.)

Immunohistochemistry (IHC)

((3.8ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)

NCBI and Uniprot Product Information

• NCBI GI #: 4506407
• NCBI GeneID: 5902
• NCBI Accession #: NP_002873.1
• NCBI GenBank Nucleotide #: NM_002882.3
• Molecular Weight: 23,239 Da
• NCBI Official Full Name: ran-specific GTPase-activating protein isoform 2
• NCBI Official Synonym Full Names: RAN binding protein 1
• NCBI Official Symbol: RANBP1
• NCBI Official Synonym Symbols: HTF9A
• NCBI Protein Information: ran-specific GTPase-activating protein
• UniProt Protein Name: Ran-specific GTPase-activating protein
• Protein Family: Ran-specific GTPase-activating protein
• UniProt Gene Name: RANBP1
• UniProt Synonym Gene Names: RanBP1
• UniProt Entry Name: RANG_HUMAN

NCBI Description

This gene encodes a protein that forms a complex with Ras-related nuclear protein (Ran) and metabolizes guanoside triphosphate (GTP). This complex participates in the regulation of the cell cycle by controlling transport of proteins and nucleic acids into the nucleus. There are multiple pseudogenes for this gene on chromosomes 9, 12, 17, and X. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]

Uniprot Description

RANBP1: a Ran-binding protein that functions in nuclear trafficking, mitosis, chromosomal segregation, and ciliogenesis. Controls both nuclear import and export. Contains a Ran binding domain and a C-terminal nuclear export signal. Following export from the nucleus, forms a complex with RanGTP and XPO1/cargo, leading to dissociation of cargo from XPO1. Stimulates RanGAP1 association with RanGTP, facilitating RanGTP hydrolysis to RanGDP. Stabilizes and regulates the formation of a RanGDP-importin/NLS receptor-RanBP1 complex during nuclear import. Controls RanGTP distribution along mitotic microtubules, which localizes critical factors, such as cyclin B1 and DLG7, to mitotic microtubles and regulates chromosome segregation. Knock down or overexpression experiments affect cellular ciliogenesis by regulating the local RanGTP concentation at the base of cilia.Belongs to the RANBP1 family.

Protein type: Cell cycle regulation; G protein regulator, misc.; Microtubule-binding

Chromosomal Location of Human Ortholog: 22q11.21

Cellular Component: centrosome; cytoplasm; nuclear envelope; nucleus

Molecular Function: GDP-dissociation inhibitor activity; GTPase activator activity; Ran GTPase binding

Biological Process: G1/S transition of mitotic cell cycle; positive regulation of GTPase activity; positive regulation of mitotic centrosome separation; protein import into nucleus; regulation of catalytic activity; RNA export from nucleus; signal transduction; spindle organization and biogenesis; ubiquitin-dependent protein catabolic process; viral reproduction