Western Blot (WB)
(Western blot analysis of 50 ug of whole cell lysate (A: Mouse brain) using a 12 % SDS PAGE gel and Ran BP1 antibody at a dilution of 1:1000)
Rabbit Ran BP1 Polyclonal Antibody | anti-Ran BP1 antibody
Ran BP1 antibody
Applications
Immunofluorescence, Immunocytochemistry, Western Blot
Purity
Ran BP1 antibody was purified by antigen-affinity chromatography
Synonyms
Ran BP1; Polyclonal Antibody; Ran BP1 antibody; Polyclonal Ran BP1; Anti-Ran BP1; HTF9A; Ran BP-1; ran-specific GTPase-activating protein; MGC88701; ran-binding protein 1; Ran BP 1; RAN binding protein 1; RANBP1; anti-Ran BP1 antibody
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Ran BP1 antibody was purified by antigen-affinity chromatography
Form/Format
Liquid in 100mM Tris, pH 7.0, with 100mM Glycine, 10% Glycerol, and 0.01% Thimersol as preservative.
Concentration
1 mg/ml (varies by lot)
Sequence Length
209
Applicable Applications for anti-Ran BP1 antibody
Immunofluorescence (IF), Immunocytochemistry (ICC), Western Blot (WB)
Application Notes
IF:1:100-1:1,000
ICC:1:100-1:1,000
WB: 1:500-1:3000
Optimal dilutions should be determined by the researcher.
ICC:1:100-1:1,000
WB: 1:500-1:3000
Optimal dilutions should be determined by the researcher.
Biological Significance
Ran/TC4-binding protein, RanBP1, interacts specifically with GTP-charged RAN. RANBP1 encodes a 23-kD protein that binds to RAN complexed with GTP but not GDP. RANBP1 does not activate GTPase activity of RAN but does markedly increase GTP hydrolysis by the RanGTPase-activating protein (RanGAP1). The RANBP1 cDNA encodes a 201-amino acid protein that is 92% similar to its mouse homolog. In both mammalian cells and in yeast, RANBP1 acts as a negative regulator of RCC1 by inhibiting RCC1-stimulated guanine nucleotide release from RAN.
Cross-Reactivity
Human, Mouse
Immunogen
Recombinant protein encompassing a proprietary sequence within the center region of human Ran BP1.
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
Western Blot (WB)
(Western blot analysis of 50 ug of whole cell lysate (A: Mouse brain) using a 12 % SDS PAGE gel and Ran BP1 antibody at a dilution of 1:1000)
Western Blot (WB)
(Western blot analysis of 50 ug of whole cell lysate (A: Mouse brain) using a 12 % SDS PAGE gel and Ran BP1 antibody at a dilution of 1:1000)
Western Blot (WB)
(Western blot analysis of 30 ug of whole cell lysate (A: HeLa S3) using a 12 % SDS PAGE gel and Ran BP1 antibody at a dilution of 1:1000)
Western Blot (WB)
(Western blot analysis of 30 ug of whole cell lysate (A: HeLa S3) using a 12 % SDS PAGE gel and Ran BP1 antibody at a dilution of 1:1000)
Immunofluorescence (IF)
(Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Ran BP-1 antibody at 1:200 dilution.)
Immunofluorescence (IF)
(Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Ran BP-1 antibody at 1:200 dilution.)
Related Product Information for anti-Ran BP1 antibody
Affinity purified Rabbit polyclonal Ran BP1 antibody
Product Categories/Family for anti-Ran BP1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
23 kDa
NCBI Official Full Name
RAN binding protein 1
NCBI Official Synonym Full Names
RAN binding protein 1
NCBI Official Symbol
ranbp1
NCBI Protein Information
RAN binding protein 1
Research Articles on Ran BP1
Similar Products
Product Notes
The Ran BP1 (Catalog #AAA835311) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Ran BP1 can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Immunocytochemistry (ICC), Western Blot (WB). IF:1:100-1:1,000 ICC:1:100-1:1,000 WB: 1:500-1:3000 Optimal dilutions should be determined by the researcher. Researchers should empirically determine the suitability of the Ran BP1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Ran BP1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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