Rabbit Annexin VII Polyclonal Antibody | anti-ANXA7 antibody

Annexin VII antibody

Cat. Num. AAA835586

• Gene Names: ANXA7; SNX; ANX7; SYNEXIN
• Applications: Immunohistochemistry, Western Blot
• Purity: Annexin VII antibody was purified by antigen-affinity chromatography
• Synonyms: Annexin VII; Polyclonal Antibody; Annexin VII antibody; Polyclonal Annexin VII; Anti-Annexin VII; ANXA7; ANX7; SYNEXIN; SNX; annexin-7; annexin A7; annexin VII; anti-ANXA7 antibody

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Annexin VII antibody was purified by antigen-affinity chromatography
• Form/Format: Supplied as a concentrated soloution containing 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
• Concentration: 0.28 mg/ml (varies by lot)
• Sequence Length: 488

• Applicable Applications for anti-ANXA7 antibody: Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
• Application Notes: IHC-P: 1:100-1:500; WB: 1:500-1:3000
• Biological Significance: Annexin VII is a member of the annexin family of calcium-dependent phospholipid binding proteins.The Annexin VII gene contains 14 exons and spans approximately 34 kb of DNA. An alternatively spliced cassette exon results in two mRNA transcripts of 2.0 and 2.4 kb which are predicted to generate two protein isoforms differing in their N-terminal domain. The alternative splicing event is tissue specific and the mRNA containing the cassette exon is prevalent in brain, heart and skeletal muscle. The transcripts also differ in their 3'-non coding regions by the use of two alternative poly(A) signals. Annexin VII encodes a protein with a molecular weight of approximately 51 kDa with a unique, highly hydrophobic N-terminal domain of 167 amino acids and a conserved C-terminal region of 299 amino acids. The latter domain is composed of alternating hydrophobic and hydrophilic segments.
• Cross-Reactivity: Human
• Immunogen: Annexin VII antibody was raised in rabbit using a recombinant fragment corresponding to a region within amino acids 122 and 459 of Annexin VII
• Preparation and Storage: Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.

Immunohistochemistry (IHC)

(Immunohistochemical staining of paraffin-embedded Cal27 xenograft using Annexin VII antibody at a dilution of 1:100)

Western Blot (WB)

(Western blot analysis of 30 ug of whole cell lysate (A:293T; B:A431) using a 7.5 % SDS PAGE gel and Annexin VII antibody at a dilution of 1:2000)

Related Product Information for anti-ANXA7 antibody

Affinity purified Rabbit polyclonal Annexin VII antibody

Product Categories/Family for anti-ANXA7 antibody

Nutrition & Metabolism; Purified Polyclonal Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 4809279
• NCBI GeneID: 310
• NCBI Accession #: NP_004025.1
• NCBI GenBank Nucleotide #: NM_004034.2
• UniProt Accession #: P20073; Q5F2H3; Q5T0M6; Q5T0M7
• Molecular Weight: 53 kDa
• NCBI Official Full Name: annexin A7 isoform 2
• NCBI Official Synonym Full Names: annexin A7
• NCBI Official Symbol: ANXA7
• NCBI Official Synonym Symbols: SNX; ANX7; SYNEXIN
• NCBI Protein Information: annexin A7
• UniProt Protein Name: Annexin A7
• Protein Family: Annexin
• UniProt Gene Name: ANXA7
• UniProt Synonym Gene Names: ANX7; SNX
• UniProt Entry Name: ANXA7_HUMAN

NCBI Description

Annexin VII is a member of the annexin family of calcium-dependent phospholipid binding proteins.The Annexin VII gene contains 14 exons and spans approximately 34 kb of DNA. An alternatively spliced cassette exon results in two mRNA transcripts of 2.0 and 2.4 kb which are predicted to generate two protein isoforms differing in their N-terminal domain. The alternative splicing event is tissue specific and the mRNA containing the cassette exon is prevalent in brain, heart and skeletal muscle. The transcripts also differ in their 3'-non coding regions by the use of two alternative poly(A) signals. Annexin VII encodes a protein with a molecular weight of approximately 51 kDa with a unique, highly hydrophobic N-terminal domain of 167 amino acids and a conserved C-terminal region of 299 amino acids. The latter domain is composed of alternating hydrophobic and hydrophilic segments. Structural analysis of the protein suggests that Annexin VII is a membrane binding protein with diverse properties, including voltage-sensitive calcium channel activity, ion selectivity and membrane fusion. [provided by RefSeq, Jul 2008]

Uniprot Description

ANXA7: Calcium/phospholipid-binding protein which promotes membrane fusion and is involved in exocytosis. Interacts with PDCD6. Isoform 1 is expressed in brain, heart and skeletal muscle. Isoform 2 is more abundant in liver, lung, kidney, spleen, fibroblasts and placenta. Belongs to the annexin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Lipid-binding; Calcium-binding; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 10q22.2

Cellular Component: endoplasmic reticulum membrane; membrane; plasma membrane; nuclear envelope; cytosol; nucleus

Molecular Function: integrin binding; protein binding; calcium-dependent phospholipid binding; calcium ion binding; calcium-dependent protein binding

Biological Process: cellular calcium ion homeostasis; regulation of cell shape; cell proliferation; cellular water homeostasis; epithelial cell differentiation; hemostasis; autophagy; social behavior; response to calcium ion; response to organic cyclic substance; response to salt stress