HIST3H2A / Histone H2A Recombinant Protein | HIST3H2A recombinant protein

Recombinant Human HIST3H2A / Histone H2A Protein

Cat. Num. AAA2546009

• Purity: > 90 % as determined by SDS-PAGE
• Synonyms: HIST3H2A / Histone H2A; Recombinant Human HIST3H2A / Histone H2A Protein; MGC3165; HIST3H2A recombinant protein

• Host: E Coli
• Purity/Purification: > 90 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 20mM beta-Mercaptoethanol, pH 6.9
• Sequence Length: 130

• Application Notes: The recombinant human HIST3H2A consists of 130 amino acids and has a calculated molecular mass of 14.2 kDa as estimated in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Met 1
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for HIST3H2A recombinant protein

Background: Histones are a complex family of highly conserved basic proteins responsible for packaging chromosomal DNA into nucleosomes. There are subtype diversities: H1, H2A, H2B and H3 or H4. It has become more and more evident that histone modifications are key players in the regulation of chromatin states and dynamics as well as in gene expression. Therefore, histone modifications and the enzymatic machineries that set them are crucial regulators that can control cellular proliferation, differentiation, plasticity, and malignancy processes. However, extracellular histones are a double-edged sword because they also damage host tissue and may cause death. Histones bound to platelets, induced calcium influx, and recruited plasma adhesion proteins such as fibrinogen to induce platelet aggregation. Histone cluster 3, H2a also known as histone H2A (HIST3H2A) is a member of histones. Covalent modification of histones is important in regulating chromatin dynamics and transcription. One example of such modification is ubiquitination, which mainly occurs on histones H2A and H2B. E3 ubiquitin ligase complex is specific for histone H2A (HIST3H2A). Reducing the expression of Ring2 results in a dramatic decrease in the level of ubiquitinated H2A in HeLa cells. DNA damage induces monoubiquitylation of histone H2A (HIST3H2A) in the vicinity of DNA lesions.

Description: A DNA sequence encoding the human HIST3H2A (NP_254280.1) (Met 1-Lys 130) was expressed and purified.

NCBI and Uniprot Product Information

• NCBI GI #: 15617199
• NCBI GeneID: 92815
• NCBI Accession #: NP_254280.1
• NCBI GenBank Nucleotide #: NM_033445.2
• UniProt Accession #: Q7L7L0; B2R4S4
• Molecular Weight: 14,121 Da
• NCBI Official Full Name: histone H2A type 3
• NCBI Official Synonym Full Names: histone cluster 3 H2A
• NCBI Official Symbol: HIST3H2A
• NCBI Protein Information: histone H2A type 3
• UniProt Protein Name: Histone H2A type 3
• Protein Family: Histone
• UniProt Gene Name: HIST3H2A

NCBI Description

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H2A family. Transcripts from this gene contain a palindromic termination element. [provided by RefSeq, Aug 2015]

Uniprot Description

HIST3H2A: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Belongs to the histone H2A family.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 1q42.13

Molecular Function: DNA binding

Biological Process: chromatin silencing; nucleosome disassembly