Rabbit Raf1 Polyclonal Antibody | anti-Raf1 antibody

Raf1 Antibody

Cat. Num. AAA840842

• Gene Names: RAF1; NS5; CRAF; Raf-1; c-Raf; CMD1NN
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunoprecipitation, Immunofluorescence, Immunohistochemistry
• Purity: Affinity purified
• Synonyms: Raf1; Polyclonal Antibody; Raf1 Antibody; RAF1; cRaf; c-Raf; RAF; CRAF; C-RAF; anti-Raf1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity purified
• Form/Format: Liquid; 100 ug (0.5 mg/ml) of antibody in PBS pH 7.2, 0.01 % BSA, 0.03 % ProClin®, and 50 % glycerol.
• Concentration: 0.2 mg/ml (varies by lot)
• Sequence Length: 648

• Applicable Applications for anti-Raf1 antibody: Western Blot (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (IHC)
• Application Notes: Western blot analysis (1-4 ug/ml), immunoprecipitation (15-25 ug/ml) and Immunohistochemistry (10-20 ug/ml). However, the optimal conditions should be determined individually. The antibody detects a 74 kDa Raf-1 protein on SDS-PAGE immunoblots. It is non cross-reactive with Raf-A and Raf-B.
• Immunogen: Synthetic peptide mapping to the C-terminus of human Raf-1
• Handling: The antibody solution should be gently mixed before use.
• Preparation and Storage: At -20°C; Shelf Life: 12 months

Western Blot

(Western Blot with Raf-1 antibody (MBS840842), using rat kidney lysate.)

Related Product Information for anti-Raf1 antibody

Background: Human Raf1 is a proto-oncogene-encoded 74 kDa kinase that serves as an upstream activator of Mek1 by phosphorylating Ser-218 and Ser-222 in the MAP kinase kinase (Mek1). It has also been implicated in the phosphorylation and activation of the cdc2 tyrosine phosphatase cdc25. Raf1 binds to the GTP-bound form of the Ras G-protein via an N-terminal domain in Raf-1. Raf-1 is activated upon phosphorylation by protein kinase C, Src and ceramide-activated protein kinase. Raf-1 is highly related to the A-Raf and B-Raf isoforms within the N-terminal G-protein binding domain (CR1), phosphorylation site domain (CR2) and the catalytic domain (CR3). Raf-1 physically interacts with Ras, RapIB, MEK1, the 14-3-3 family of proteins and the molecular chaperones hsp90 and p50.

NCBI and Uniprot Product Information

• NCBI GI #: 4506401
• NCBI GeneID: 5894
• NCBI Accession #: NP_002871.1
• NCBI GenBank Nucleotide #: NM_002880.3
• UniProt Accession #: P04049; Q15278; Q9UC20; B0LPH8; B2R5N3
• NCBI Official Full Name: RAF proto-oncogene serine/threonine-protein kinase
• NCBI Official Synonym Full Names: Raf-1 proto-oncogene, serine/threonine kinase
• NCBI Official Symbol: RAF1
• NCBI Official Synonym Symbols: NS5; CRAF; Raf-1; c-Raf; CMD1NN
• NCBI Protein Information: RAF proto-oncogene serine/threonine-protein kinase
• UniProt Protein Name: RAF proto-oncogene serine/threonine-protein kinase
• Protein Family: Rik1-associated factor
• UniProt Gene Name: RAF1
• UniProt Synonym Gene Names: RAF; cRaf
• UniProt Entry Name: RAF1_HUMAN

NCBI Description

This gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2. [provided by RefSeq, Jul 2008]

Uniprot Description

RAF1: a proto-oncogenic TKL kinase of the RAF family. Functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated Raf-1 can phosphorylate and activate MEK1/2, which in turn phosphorylate and activate ERK1/2. Acts as a regulatory link between the membrane-associated Ras GTPases and the MAPK/ERK cascade, and this critical regulatory link functions as a switch determining cell fate decisions including proliferation, differentiation, apoptosis, survival and oncogenic transformation. RAF1 activation initiates a mitogen-activated protein kinase (MAPK) cascade that comprises a sequential phosphorylation of the dual-specific MAPK kinases (MEK1/2) and the extracellular signal-regulated kinases (ERK1/2). RAF1, subsequent to phosphorylation by PAK1, phosphorylates BAD (Bcl2-antagonist of cell death) at S75. Phosphorylates adenylyl cyclases ADCY2, ADCY5 and ADCY6, resulting in their activation. Phosphorylates PPP1R12A resulting in inhibition of the phosphatase activity. Phosphorylates TNNT2 (cardiac muscle troponin T). Can promote NF-kB activation and inhibit signal transducers involved in motility (ROCK2), apoptosis (ASK1 and MST2), proliferation and angiogenesis (RB1). Can protect cells from apoptosis also by translocating to the mitochondria where it binds BCL2 and displaces BAD. Restricts caspase activation in response to selected stimuli, notably Fas stimulation, pathogen-mediated macrophage apoptosis, and erythroid differentiation.

Protein type: Protein kinase, TKL; EC 2.7.11.1; Kinase, protein; Oncoprotein; Protein kinase, Ser/Thr (non-receptor); TKL group; RAF family

Chromosomal Location of Human Ortholog: 3p25

Cellular Component: Golgi apparatus; mitochondrial outer membrane; cytoplasm; plasma membrane; pseudopodium; nucleus; cytosol

Molecular Function: small GTPase binding; protein serine/threonine kinase activity; identical protein binding; protein binding; MAP kinase kinase kinase activity; protein heterodimerization activity; mitogen-activated protein kinase kinase binding; metal ion binding; ATP binding; protein kinase activity

Biological Process: axon guidance; activation of MAPKK activity; wound healing; nerve growth factor receptor signaling pathway; apoptosis; somatic stem cell maintenance; heart development; intermediate filament cytoskeleton organization and biogenesis; negative regulation of caspase activity; signal transduction; protein amino acid phosphorylation; regulation of apoptosis; synaptic transmission; negative regulation of cell proliferation; small GTPase mediated signal transduction; regulation of cell differentiation; transmembrane transport; epidermal growth factor receptor signaling pathway; platelet activation; fibroblast growth factor receptor signaling pathway; adenylate cyclase activation; MAPKKK cascade; positive regulation of peptidyl-serine phosphorylation; cell proliferation; Ras protein signal transduction; regulation of Rho protein signal transduction; response to hypoxia; insulin receptor signaling pathway; innate immune response; vascular endothelial growth factor receptor signaling pathway; blood coagulation; negative regulation of protein complex assembly; negative regulation of apoptosis

Disease: Cardiomyopathy, Dilated, 1nn; Noonan Syndrome 5; Leopard Syndrome 2