Rabbit anti-Human RAD9 Polyclonal Antibody | anti-RAD9 antibody

RAD9 (Phospho-Ser272) Antibody

Cat. Num. AAA9416499

• Gene Names: RAD9A; RAD9
• Reactivity: Human
• Applications: Western Blot
• Purity: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
• Synonyms: RAD9; Polyclonal Antibody; RAD9 (Phospho-Ser272) Antibody; anti-RAD9 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: RAD9 (Phospho-Ser272) Antibody detects endogenous levels of RAD9 only when phosphorylated at Ser272
• Purity/Purification: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
• Form/Format: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 315

• Applicable Applications for anti-RAD9 antibody: Western Blot (WB)
• Application Notes: Western Blot: 1:1000
• Immunogen: A synthesized peptide derived from human RAD9 (Phospho-Ser272)
• Immunogen Type: Peptide
• Modification: Phospho-Ser272
• Preparation and Storage: Store at -20 degree C

Western Blot (WB)

(Western blot analysis RAD9 (Phospho-Ser272) using H2O2 treated K562 whole cell lysates)

Product Categories/Family for anti-RAD9 antibody

Phospho-specific Ab

NCBI and Uniprot Product Information

• NCBI GI #: 342672046
• NCBI GeneID: 5883
• NCBI Accession #: NP_001230153.1
• NCBI GenBank Nucleotide #: NM_001243224.1
• UniProt Accession #: Q96C41; Q6FI29; B2RCZ8; Q99638
• Molecular Weight: 42,547 Da
• NCBI Official Full Name: cell cycle checkpoint control protein RAD9A isoform 2
• NCBI Official Synonym Full Names: RAD9 checkpoint clamp component A
• NCBI Official Symbol: RAD9A
• NCBI Official Synonym Symbols: RAD9
• NCBI Protein Information: cell cycle checkpoint control protein RAD9A
• UniProt Protein Name: Cell cycle checkpoint control protein RAD9A
• Protein Family: RAD9, HUS1, RAD1-interacting nuclear orphan protein
• UniProt Gene Name: RAD9A
• UniProt Synonym Gene Names: hRAD9
• UniProt Entry Name: RAD9A_HUMAN

NCBI Description

This gene product is highly similar to Schizosaccharomyces pombe rad9, a cell cycle checkpoint protein required for cell cycle arrest and DNA damage repair. This protein possesses 3' to 5' exonuclease activity, which may contribute to its role in sensing and repairing DNA damage. It forms a checkpoint protein complex with RAD1 and HUS1. This complex is recruited by checkpoint protein RAD17 to the sites of DNA damage, which is thought to be important for triggering the checkpoint-signaling cascade. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]

Uniprot Description

RAD9A: a cell cycle checkpoint protein required for cell cycle arrest and DNA damage repair in response to DNA damage. Possesses a 3' to 5' exonuclease activity, which may contribute to its role in sensing and repairing DNA damage. Forms a checkpoint protein complex with RAD1 and HUS1. This complex is recruited by checkpoint protein RAD17 to the sites of DNA damage, which is thought to be important for triggering the checkpoint-signaling cascade. Use of alternative polyA sites has been noted for Rad9.

Protein type: Apoptosis; Cell cycle regulation; DNA replication; Deoxyribonuclease; EC 3.1.11.2

Chromosomal Location of Human Ortholog: 11q13.1-q13.2

Cellular Component: nucleoplasm; nucleus

Molecular Function: 3'-5' exonuclease activity; enzyme binding; histone deacetylase binding; protein binding; protein kinase binding; SH3 domain binding

Biological Process: DNA damage checkpoint; DNA replication; DNA replication checkpoint; intra-S DNA damage checkpoint; response to DNA damage stimulus