Rabbit RAD9 Polyclonal Antibody | anti-RAD9A antibody

RAD9 Antibody (Center L265)

Cat. Num. AAA9210947

• Gene Names: RAD9A; RAD9
• Reactivity: Human (Predicted Reactivity: Monkey)
• Applications: Western Blot, ELISA
• Purity: Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: RAD9; Polyclonal Antibody; RAD9 Antibody (Center L265); Cell cycle checkpoint control protein RAD9A; hRAD9; DNA repair exonuclease rad9 homolog A; RAD9A; anti-RAD9A antibody

• Host: Rabbit
• Reactivity: Human (Predicted Reactivity: Monkey)
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This RAD9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 250-279 amino acids from the Central region of human RAD9.
• Purity/Purification: Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Concentration: Vial Concentration: 0.35 (varies by lot)
• Sequence Positions: 250-279
• Sequence Length: 315

• Applicable Applications for anti-RAD9A antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB~~1:1000
• Antigen Type: Synthetic Peptide
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Western blot analysis of anti-Rad9 Antibody (Center L265) in Hela cell line lysates (35ug/lane). Rad9 (arrow) was detected using the purified Pab.)

Related Product Information for anti-RAD9A antibody

Rad9 is highly similar to Schizosaccharomyces pombe rad9, a cell cycle checkpoint protein required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein is found to possess 3' to 5' exonuclease activity, which may contribute to its role in sensing and repairing DNA damage. It forms a checkpoint protein complex with RAD1 and HUS1. This complex is recruited by checkpoint protein RAD17 to the sites of DNA damage, which is thought to be important for triggering the checkpoint-signaling cascade.

References

Maniwa, Y., et al., Cancer 103(1):126-132 (2005).
Wang, W., et al., Proc. Natl. Acad. Sci. U.S.A. 101(48):16762-16767 (2004).
Lindsey-Boltz, L.A., et al., (er) Nucleic Acids Res. 32(15):4524-4530 (2004).
Toueille, M., et al., (er) Nucleic Acids Res. 32(11):3316-3324 (2004).
Loegering, D., et al., J. Biol. Chem. 279(18):18641-18647 (2004).

NCBI and Uniprot Product Information

• NCBI GI #: 342672046
• NCBI GeneID: 5883
• NCBI Accession #: NP_001230153.1; NP_004575.1
• NCBI GenBank Nucleotide #: NM_001243224.1
• UniProt Accession #: Q99638; Q6FI29; Q96C41; B2RCZ8
• Molecular Weight: 42547
• NCBI Official Full Name: cell cycle checkpoint control protein RAD9A isoform 2
• NCBI Official Synonym Full Names: RAD9 checkpoint clamp component A
• NCBI Official Symbol: RAD9A
• NCBI Official Synonym Symbols: RAD9
• NCBI Protein Information: cell cycle checkpoint control protein RAD9A
• UniProt Protein Name: Cell cycle checkpoint control protein RAD9A
• Protein Family: RAD9, HUS1, RAD1-interacting nuclear orphan protein
• UniProt Gene Name: RAD9A
• UniProt Synonym Gene Names: hRAD9
• UniProt Entry Name: RAD9A_HUMAN

NCBI Description

This gene product is highly similar to Schizosaccharomyces pombe rad9, a cell cycle checkpoint protein required for cell cycle arrest and DNA damage repair. This protein possesses 3' to 5' exonuclease activity, which may contribute to its role in sensing and repairing DNA damage. It forms a checkpoint protein complex with RAD1 and HUS1. This complex is recruited by checkpoint protein RAD17 to the sites of DNA damage, which is thought to be important for triggering the checkpoint-signaling cascade. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]

Uniprot Description

Rad9: a cell cycle checkpoint protein required for cell cycle arrest and DNA damage repair in response to DNA damage. Possesses a 3' to 5' exonuclease activity, which may contribute to its role in sensing and repairing DNA damage. Forms a checkpoint protein complex with RAD1 and HUS1. This complex is recruited by checkpoint protein RAD17 to the sites of DNA damage, which is thought to be important for triggering the checkpoint-signaling cascade. Use of alternative polyA sites has been noted for Rad9.

Protein type: Deoxyribonuclease; EC 3.1.11.2; Apoptosis; DNA replication; Cell cycle regulation

Chromosomal Location of Human Ortholog: 11q13.1-q13.2

Cellular Component: nucleoplasm; cytoplasm; nucleus

Molecular Function: protein binding; enzyme binding; histone deacetylase binding; exodeoxyribonuclease III activity; 3'-5' exonuclease activity; protein kinase binding; SH3 domain binding

Biological Process: intra-S DNA damage checkpoint; DNA damage checkpoint; DNA catabolic process, exonucleolytic; DNA repair; DNA replication; DNA replication checkpoint; response to DNA damage stimulus