Rat anti-Human CD200 Antibody | anti-CD200 antibody

Anti-Human CD200, Purified, (Clone: 1B9) (Rat IgG2a)

Cat. Num. AAA520896

• Gene Names: CD200; MRC; MOX1; MOX2; OX-2
• Reactivity: Human
• Applications: ELISA, Flow Cytometry
• Synonyms: CD200; Anti-Human CD200; Purified; (Clone: 1B9) (Rat IgG2a); anti-CD200 antibody

• Host: Rat
• Reactivity: Human
• Isotype: IgG_2a
• Clone Number: 1B9
• Specificity: This antibody is specific for human CD200.
• Form/Format: Purified
• Concentration: 1.0 mg/ml (varies by lot)

• Applicable Applications for anti-CD200 antibody: ELISA (EIA), Flow Cytometry (FC)
• Application Notes: Tissue Distribution by Flow Cytometry Analysis:; Cell Concentration: 1x10⁶ cells per tests; Antibody Concentration Used: 0.1 ug/10⁶ cells; ; Cell Source: Human PBLs; ; Percentage of cells stained above control: 16.4 %; ; *For optimal results in various applications, it is recommended that each investigator determine dilutions appropriate for individual use.
• Immunogen: Human CD200-Fc fusion protein.
• Conjugate: Unconjugated
• Presentation: Purified: Purified IgG buffered in PBS AND 0.02% NaN₃. (Purified via Protein G Chromatography). For maximum recovery of contents, spin down tube before use.; LE: Purified Ig buffered in PBS, no preservative, 0.2um sterile filtered. (Purified from cell culture supernatant via Protein G Chromataopgraphy); Biotin, FITC, PE, APC, AF488 and AF647: Biotin/FITC/PE/APC/AF488/AF647 conjugated IgG buffered in PBS, 0.02% NaN₃ and EIA grade BSA as a stabiizing protein to bring total protein concentration to 4-5 mg/ml.
• Preparation and Storage: Store at 4°C.; DO NOT FREEZE APC, PE, AF488, and AF647 conjugates. ; For long term storage (Purified, LE, Biotin and FITC), aliquot and freeze unused portion -20°C in volumes appropriate for single usage. ; Avoid freeze/thaw cycles.

Flow Cytometry

(Human peripheral blood lymphocytes were stained with anti-CD200 (clone: 1B9) (blue histogram) or Rat IgG2a isotype control (red histogram).)

Related Product Information for anti-CD200 antibody

CD200, also known as OX-2, is a type-1 membrane glycoprotein that belongs to the immunoglobin superfamily (IgSF). It's expressed on several tissues including thymocytes, neurons, endothelium, follicular dendritic cells in all lymphoid organs, a subset of CD34+ progenitor cells, and at low levels on some smooth muscle and B lymphocytes. CD200 is not expressed on NK cells, monocytes, granulocytes, or platelets. CD200 co-stimulates T-cell proliferation and may regulate myeoid cell activity in a variety of tissues. Additionally, interaction of CD200 with its receptor on macrophages induces a downregulation of macrophage activity in a variety of tissues, suggesting a regulatory function.

NCBI and Uniprot Product Information

• NCBI GI #: 119600082
• NCBI GeneID: 4345
• NCBI Official Full Name: CD200 antigen, isoform CRA_a
• NCBI Official Synonym Full Names: CD200 molecule
• NCBI Official Symbol: CD200
• NCBI Official Synonym Symbols: MRC; MOX1; MOX2; OX-2
• NCBI Protein Information: OX-2 membrane glycoprotein
• Protein Family: OX-2 membrane glycoprotein

NCBI Description

This gene encodes a type I membrane glycoprotein containing two extracellular immunoglobulin domains, a transmembrane and a cytoplasmic domain. This gene is expressed by various cell types, including B cells, a subset of T cells, thymocytes, endothelial cells, and neurons. The encoded protein plays an important role in immunosuppression and regulation of anti-tumor activity. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jan 2016]