Rabbit anti-Human Rad9 Polyclonal Antibody | anti-Rad9 antibody

Rad9 (S387) (RAD9A, Cell cycle checkpoint control protein RAD9A, DNA repair exonuclease rad9 homolog A) (AP)

Cat. Num. AAA6340064

• Gene Names: RAD9A; RAD9
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: Rad9; Polyclonal Antibody; Rad9 (S387) (RAD9A; Cell cycle checkpoint control protein RAD9A; DNA repair exonuclease rad9 homolog A) (AP); RAD9A; DNA repair exonuclease rad9 homolog A; anti-Rad9 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-Rad9 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:10-50; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: RAD9A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the human RAD9A.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Rad9 Antibody (pS387) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the Rad9 antibody detected the Rad9 protein (arrow).)

Immunohistochemistry (IHC)

(Rad9 Antibody (S387) immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Rad9 Antibody (S387) for immunohistochemistry. Clinical relevance has not been evaluated.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of Rad9 Antibody (S387) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

Related Product Information for anti-Rad9 antibody

This gene product is highly similar to Schizosaccharomyces pombe rad9, a cell cycle checkpoint protein required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein is found to possess 3' to 5' exonuclease activity, which may contribute to its role in sensing and repairing DNA damage. It forms a checkpoint protein complex with RAD1 and HUS1. This complex is recruited by checkpoint protein RAD17 to the sites of DNA damage, which is thought to be important for triggering the checkpoint-signaling cascade. Use of alternative polyA sites has been noted for this gene.

Product Categories/Family for anti-Rad9 antibody

Antibodies; DNA Replication; Repair

References

Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). Takeishi, Y., et al. Genes Cells 15(7):761-771(2010). Greer Card, D.A., et al. J. Biol. Chem. 285(20):15653-15661(2010). Bai, H., et al. DNA Repair (Amst.) 9(5):478-487(2010). Sierant, M.L., et al. Cell Cycle 9(3):548-556(2010).

NCBI and Uniprot Product Information

• NCBI GI #: 4759022
• NCBI GeneID: 5883
• NCBI Accession #: NP_004575.1
• NCBI GenBank Nucleotide #: NM_004584.2
• UniProt Accession #: Q99638; Q6FI29; Q96C41; B2RCZ8
• Molecular Weight: 42,547 Da
• NCBI Official Full Name: cell cycle checkpoint control protein RAD9A isoform 1
• NCBI Official Synonym Full Names: RAD9 checkpoint clamp component A
• NCBI Official Symbol: RAD9A
• NCBI Official Synonym Symbols: RAD9
• NCBI Protein Information: cell cycle checkpoint control protein RAD9A
• UniProt Protein Name: Cell cycle checkpoint control protein RAD9A
• Protein Family: RAD9, HUS1, RAD1-interacting nuclear orphan protein
• UniProt Gene Name: RAD9A
• UniProt Synonym Gene Names: hRAD9
• UniProt Entry Name: RAD9A_HUMAN

NCBI Description

This gene product is highly similar to Schizosaccharomyces pombe rad9, a cell cycle checkpoint protein required for cell cycle arrest and DNA damage repair. This protein possesses 3' to 5' exonuclease activity, which may contribute to its role in sensing and repairing DNA damage. It forms a checkpoint protein complex with RAD1 and HUS1. This complex is recruited by checkpoint protein RAD17 to the sites of DNA damage, which is thought to be important for triggering the checkpoint-signaling cascade. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]

Uniprot Description

Rad9: a cell cycle checkpoint protein required for cell cycle arrest and DNA damage repair in response to DNA damage. Possesses a 3' to 5' exonuclease activity, which may contribute to its role in sensing and repairing DNA damage. Forms a checkpoint protein complex with RAD1 and HUS1. This complex is recruited by checkpoint protein RAD17 to the sites of DNA damage, which is thought to be important for triggering the checkpoint-signaling cascade. Use of alternative polyA sites has been noted for Rad9.

Protein type: EC 3.1.11.2; DNA replication; Deoxyribonuclease; Apoptosis; Cell cycle regulation

Chromosomal Location of Human Ortholog: 11q13.1-q13.2

Cellular Component: nucleoplasm; cytoplasm; nucleus

Molecular Function: protein binding; enzyme binding; histone deacetylase binding; exodeoxyribonuclease III activity; 3'-5' exonuclease activity; SH3 domain binding; protein kinase binding

Biological Process: intra-S DNA damage checkpoint; DNA damage checkpoint; DNA replication checkpoint; DNA replication; DNA repair; DNA catabolic process, exonucleolytic; response to DNA damage stimulus