Rabbit anti-Human RAD54 Polyclonal Antibody | anti-RAD54 antibody

RAD54, CT (RAD54L, RAD54A, DNA repair and recombination protein RAD54-like, RAD54 homolog) (PE)

Cat. Num. AAA6340063

• Gene Names: RAD54L; HR54; hHR54; RAD54A; hRAD54
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: RAD54; Polyclonal Antibody; CT (RAD54L; RAD54A; DNA repair and recombination protein RAD54-like; RAD54 homolog) (PE); RAD54L; RAD54 homolog; anti-RAD54 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-RAD54 antibody: Flow Cytometry (FC/FACS), FLISA, Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: RAD54 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 641-671 amino acids from the C-terminal region of human RAD54.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(RAD54 Antibody (C-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the RAD54 antibody detected the RAD54 protein (arrow).)

Flow Cytometry (FC/FACS)

(RAD54 Antibody (C-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-RAD54 antibody

The protein encoded by this gene belongs to the DEAD-like helicase superfamily, and shares similarity with Saccharomyces cerevisiae Rad54, a protein known to be involved in the homologous recombination and repair of DNA. This protein has been shown to play a role in homologous recombination related repair of DNA double-strand breaks. The binding of this protein to double-strand DNA induces a DNA topological change, which is thought to facilitate homologous DNA paring, and stimulate DNA recombination. Alternative splicing results in multiple transcript variants encoding the same protein.

Product Categories/Family for anti-RAD54 antibody

Antibodies; DNA Replication; Repair

References

Liu, Y., et al. Carcinogenesis 31(10):1762-1769(2010). Briggs, F.B., et al. Am. J. Epidemiol. 172(2):217-224(2010). Monsees, G.M., et al. Breast Cancer Res. Treat. (2010). In press: Guey, L.T., et al. Eur. Urol. 57(2):283-292(2010). Hosgood, H.D. III, et al. Occup Environ Med 66(12):848-853(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 216548186
• NCBI GeneID: 8438
• NCBI Accession #: NP_003570
• NCBI GenBank Nucleotide #: NM_003579
• UniProt Accession #: Q92698
• Molecular Weight: 84kDa
• NCBI Official Full Name: DNA repair and recombination protein RAD54-like
• NCBI Official Synonym Full Names: RAD54 like
• NCBI Official Symbol: RAD54L
• NCBI Official Synonym Symbols: HR54; hHR54; RAD54A; hRAD54
• NCBI Protein Information: DNA repair and recombination protein RAD54-like
• UniProt Protein Name: DNA repair and recombination protein RAD54-like
• Protein Family: DNA repair and recombination protein
• UniProt Gene Name: RAD54L
• UniProt Synonym Gene Names: RAD54A; hHR54; hRAD54
• UniProt Entry Name: RAD54_HUMAN

NCBI Description

The protein encoded by this gene belongs to the DEAD-like helicase superfamily, and shares similarity with Saccharomyces cerevisiae Rad54, a protein known to be involved in the homologous recombination and repair of DNA. This protein has been shown to play a role in homologous recombination related repair of DNA double-strand breaks. The binding of this protein to double-strand DNA induces a DNA topological change, which is thought to facilitate homologous DNA paring, and stimulate DNA recombination. Alternative splicing results in multiple transcript variants encoding the same protein.[provided by RefSeq, Dec 2008]

Uniprot Description

RAD54L: Involved in DNA repair and mitotic recombination. Functions in the recombinational DNA repair (RAD52) pathway. Dissociates RAD51 from nucleoprotein filaments formed on dsDNA. Could be involved in the turnover of RAD51 protein-dsDNA filaments. May play also an essential role in telomere length maintenance and telomere capping in mammalian cells. Belongs to the SNF2/RAD54 helicase family.

Protein type: Cell cycle regulation; DNA repair, damage; EC 3.6.4.-; Helicase; EC 3.6.1.-

Chromosomal Location of Human Ortholog: 1p32

Cellular Component: nucleoplasm; nucleus

Molecular Function: protein binding; DNA binding; helicase activity; ATP binding

Biological Process: response to drug; meiosis; DNA strand renaturation; response to ionizing radiation; DNA repair; chromosome organization and biogenesis; double-strand break repair via homologous recombination; DNA recombination

Disease: Lymphoma, Non-hodgkin, Familial; Breast Cancer