Immunohistochemistry (IHC)
Rabbit anti-Human, Mouse RAD50 Polyclonal Antibody | anti-RAD50 antibody
Anti-RAD50 Antibody
IHC: 1:50-1:200
Immunohistochemistry (IHC)
Immunohistochemistry (IHC)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is highly similar to Saccharomyces cerevisiae Rad50, a protein involved in DNA double-strand break repair. This protein forms a complex with MRE11 and NBS1. The protein complex binds to DNA and displays numerous enzymatic activities that are required for nonhomologous joining of DNA ends. This protein, cooperating with its partners, is important for DNA double-strand break repair, cell cycle checkpoint activation, telomere maintenance, and meiotic recombination. Knockout studies of the mouse homolog suggest this gene is essential for cell growth and viability. Mutations in this gene are the cause of Nijmegen breakage syndrome-like disorder.[provided by RefSeq, Apr 2010]
Uniprot Description
RAD50: a component of the Mre11-Rad50-NBS1 (MRN) complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11A. RAD50 may be required to bind DNA ends and hold them in close proximity. This could facilitate searches for short or long regions of sequence homology in the recombining DNA templates, and may also stimulate the activity of DNA ligases and/or restrict the nuclease activity of MRE11A to prevent nucleolytic degradation past a given point. The complex may also be required for DNA damage signaling via activation of the ATM kinase. In telomeres the MRN complex may modulate t-loop formation. Three alternatively spliced isoforms have been described.
Protein type: Adaptor/scaffold; DNA repair, damage; EC 3.6.-.-; Hydrolase
Chromosomal Location of Human Ortholog: 5q31.1
Cellular Component: condensed nuclear chromosome; membrane; Mre11 complex; nuclear chromatin; nuclear chromosome, telomeric region; nucleoplasm
Molecular Function: 3'-5' exonuclease activity; adenylate kinase activity; ATP-dependent DNA helicase activity; DNA binding; double-stranded telomeric DNA binding; G-quadruplex DNA binding; protein binding; protein binding, bridging; single-stranded DNA specific endodeoxyribonuclease activity; single-stranded telomeric DNA binding
Biological Process: DNA double-strand break processing; DNA duplex unwinding; DNA recombination; DNA repair; DNA replication; DNA synthesis during DNA repair; double-strand break repair; double-strand break repair via homologous recombination; double-strand break repair via nonhomologous end joining; meiotic recombination; positive regulation of kinase activity; positive regulation of protein amino acid autophosphorylation; positive regulation of telomere maintenance; regulation of mitotic recombination; response to DNA damage stimulus; strand displacement; telomere maintenance; telomere maintenance via recombination; telomere maintenance via telomerase; telomeric 3' overhang formation
Disease: Nijmegen Breakage Syndrome-like Disorder
Research Articles on RAD50
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Product Notes
The RAD50 rad50 (Catalog #AAA4150237) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-RAD50 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's RAD50 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500-1:2000 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the RAD50 rad50 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RAD50, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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