Rabbit RACK1 Polyclonal Antibody | anti-RACK1 antibody

RACK1 Rabbit pAb

Cat. Num. AAA4752208

• Gene Names: GNB2L1; H12.3; HLC-7; PIG21; RACK1; Gnb2-rs1
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: Affinity purified
• Synonyms: RACK1; Polyclonal Antibody; RACK1 Rabbit pAb; Receptor of activated protein C kinase 1; H12.3; HLC-7; PIG21; GNB2L1; Gnb2-rs1; anti-RACK1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: In the liver, expressed at higher levels in activated hepatic stellate cells than in hepatocytes or Kupffer cells. Up-regulated in hepatocellular carcinomas and in the adjacent non-tumor liver tissue.
• Purity/Purification: Affinity purified
• Form/Format: Supplied in 50nM Tris-Glycine (pH7.4), 0.15M Nacl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

• Applicable Applications for anti-RACK1 antibody: Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
• Application Notes: WB: 1:1000; IHC: 1:20; FC/FACS: 1:20
• Immunogen: A synthetic peptide of human RACK1
• Conjugation: Unconjugated
• Modification: Unmodification
• Subcellular Location: Cell membrane, Cell projection, Cytoplasm, Membrane, Nucleus
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot detection of RACK1 in C6,3T3,Hela cell lysates using RACK1 Rabbit pAb(1:1000 diluted). Predicted band size:35kDa. Observed band size:35kDa.)

Related Product Information for anti-RACK1 antibody

Scaffolding protein involved in the recruitment, assembly and/or regulation of a variety of signaling molecules. Interacts with a wide variety of proteins and plays a role in many cellular processes. Component of the 40S ribosomal subunit involved in translational repression (PubMed:23636399). Involved in the initiation of the ribosome quality control (RQC), a pathway that takes place when a ribosome has stalled during translation, by promoting ubiquitination of a subset of 40S ribosomal subunits (PubMed:28132843). Binds to and stabilizes activated protein kinase C (PKC), increasing PKC-mediated phosphorylation. May recruit activated PKC to the ribosome, leading to phosphorylation of EIF6. Inhibits the activity of SRC kinases including SRC, LCK and YES1. Inhibits cell growth by prolonging the G0/G1 phase of the cell cycle. Enhances phosphorylation of BMAL1 by PRKCA and inhibits transcriptional activity of the BMAL1-CLOCK heterodimer. Facilitates ligand-independent nuclear translocation of AR following PKC activation, represses AR transactivation activity and is required for phosphorylation of AR by SRC. Modulates IGF1R-dependent integrin signaling and promotes cell spreading and contact with the extracellular matrix. Involved in PKC-dependent translocation of ADAM12 to the cell membrane. Promotes the ubiquitination and proteasome-mediated degradation of proteins such as CLEC1B and HIF1A. Required for VANGL2 membrane localization, inhibits Wnt signaling, and regulates cellular polarization and oriented cell division during gastrulation. Required for PTK2/FAK1 phosphorylation and dephosphorylation. Regulates internalization of the muscarinic receptor CHRM2. Promotes apoptosis by increasing oligomerization of BAX and disrupting the interaction of BAX with the anti-apoptotic factor BCL2L. Inhibits TRPM6 channel activity. Regulates cell surface expression of some GPCRs such as TBXA2R. Plays a role in regulation of FLT1-mediated cell migration. Involved in the transport of ABCB4 from the Golgi to the apical bile canalicular membrane (PubMed:19674157). Promotes migration of breast carcinoma cells by binding to and activating RHOA (PubMed:20499158).

NCBI and Uniprot Product Information

• NCBI GI #: 5174447
• NCBI GeneID: 10399
• NCBI Accession #: NP_006089.1
• NCBI GenBank Nucleotide #: NM_006098.4
• UniProt Accession #: P63244; P25388; P99049; Q53HU2; Q5J8M6; Q5VLR4; Q6FH47; B3KTJ0; D3DWS0
• Molecular Weight: 317
• NCBI Official Full Name: guanine nucleotide-binding protein subunit beta-2-like 1
• NCBI Official Synonym Full Names: guanine nucleotide binding protein (G protein), beta polypeptide 2-like 1
• NCBI Official Symbol: GNB2L1
• NCBI Official Synonym Symbols: H12.3; HLC-7; PIG21; RACK1; Gnb2-rs1
• NCBI Protein Information: guanine nucleotide-binding protein subunit beta-2-like 1; lung cancer oncogene 7; proliferation-inducing gene 21; receptor for activated C kinase 1; human lung cancer oncogene 7 protein; receptor of activated protein kinase C 1; cell proliferation-inducin
• UniProt Protein Name: Guanine nucleotide-binding protein subunit beta-2-like 1
• Protein Family: Receptor of activated protein C kinase
• UniProt Gene Name: GNB2L1
• UniProt Synonym Gene Names: HLC-7; RACK1
• UniProt Entry Name: GBLP_HUMAN