SDS-PAGE
Rabbit anti-Human Karyopherin Alpha 2 (KPNa2) Polyclonal Antibody | anti-KPNa2 antibody
FITC-linked Antibody to Karyopherin Alpha 2 (KPNa2)
Immunocytochemistry in formalin fixed cells: 1:50-500
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Immunohistochemistry in paraffin section: 1:10-100
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.
SDS-PAGE
SDS-PAGE
Knockout Validation
(Knockout Validation: Lane 1: Wild-type HepG2 cell lysate;;Lane 2: KPNa2 knockout HepG2 cell lysate;;Predicted MW: 58kDa ;Observed MW: 58kDa;Primary Ab: 3ug/ml Rabbit Anti-Human KPNa2 Antibody;Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody;(#MBS2086047))
Knockout Validation
(Knockout Validation: Lane 1: Wild-type HepG2 cell lysate;;Lane 2: KPNa2 knockout HepG2 cell lysate;;Predicted MW: 58kDa ;Observed MW: 58kDa;Primary Ab: 3ug/ml Rabbit Anti-Human KPNa2 Antibody;Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody;(#MBS2086047))
NCBI and Uniprot Product Information
NCBI Description
The import of proteins into the nucleus is a process that involves at least 2 steps. The first is an energy-independent docking of the protein to the nuclear envelope and the second is an energy-dependent translocation through the nuclear pore complex. Imported proteins require a nuclear localization sequence (NLS) which generally consists of a short region of basic amino acids or 2 such regions spaced about 10 amino acids apart. Proteins involved in the first step of nuclear import have been identified in different systems. These include the Xenopus protein importin and its yeast homolog, SRP1 (a suppressor of certain temperature-sensitive mutations of RNA polymerase I in Saccharomyces cerevisiae), which bind to the NLS. KPNA2 protein interacts with the NLSs of DNA helicase Q1 and SV40 T antigen and may be involved in the nuclear transport of proteins. KPNA2 also may play a role in V(D)J recombination [provided by RefSeq, Jul 2008]
Uniprot Description
KPNA2: Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran- dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Belongs to the importin alpha family.
Protein type: Nuclear import; Karyopherin; Nucleoporin
Chromosomal Location of Human Ortholog: 17q24.2
Cellular Component: nucleoplasm; membrane; cytoplasm; cytosol
Molecular Function: protein binding; histone deacetylase binding; nuclear localization sequence binding; protein transporter activity
Biological Process: viral reproduction; cytokine and chemokine mediated signaling pathway; regulation of DNA recombination; NLS-bearing substrate import into nucleus; DNA metabolic process
Research Articles on KPNa2
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Product Notes
The KPNa2 kpna2 (Catalog #AAA2015280) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The FITC-linked Antibody to Karyopherin Alpha 2 (KPNa2) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Karyopherin Alpha 2 (KPNa2) can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry-Paraffin/Frozen (IHC-P/F), Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blot (WB). Western blotting: 1:50-400 Immunocytochemistry in formalin fixed cells: 1:50-500 Immunohistochemistry in formalin fixed frozen section: 1:50-500 Immunohistochemistry in paraffin section: 1:10-100 Enzyme-linked Immunosorbent Assay: 1:100-200 Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the KPNa2 kpna2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Karyopherin Alpha 2 (KPNa2), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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