Testing Data
(Sample (30 ug of whole cell lysate) A: A431 B: Raji 7.5% SDS PAGE Primary antibody diluted at 1: 1000)
Rabbit anti-Human RACGAP1 Polyclonal Antibody | anti-RACGAP1 antibody
RACGAP1 antibody
Immunohistochemistry: 1:100-1:500
Immunofluorescence: 1:100-1:200
Testing Data
(Sample (30 ug of whole cell lysate) A: A431 B: Raji 7.5% SDS PAGE Primary antibody diluted at 1: 1000)
Testing Data
(Sample (30 ug of whole cell lysate) A: A431 B: Raji 7.5% SDS PAGE Primary antibody diluted at 1: 1000)
Immunohistochemistry (IHC)
(A:Immunohistochemical analysis of paraffin-embedded H1299 xenograft, using RACGAP1 antibody at 1: 500 dilution. B:Immunohistochemical analysis of paraffin-embedded Hepatoma, using RACGAP1 antibody at 1: 500 dilution.)
Immunohistochemistry (IHC)
(A:Immunohistochemical analysis of paraffin-embedded H1299 xenograft, using RACGAP1 antibody at 1: 500 dilution. B:Immunohistochemical analysis of paraffin-embedded Hepatoma, using RACGAP1 antibody at 1: 500 dilution.)
Immunofluorescence (IF)
(Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed A431, using RACGAP1 antibody (Green) at 1: 500 dilution and alpha-tubulin antibody (Red) at 1: 2000.)
Immunofluorescence (IF)
(Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed A431, using RACGAP1 antibody (Green) at 1: 500 dilution and alpha-tubulin antibody (Red) at 1: 2000.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene belongs to the GTPase-activating protein (GAP) family. GAPs bind activated forms of Rho GTPases and stimulate GTP hydrolysis. Through this catalytic function, GAPs negatively regulate Rho-mediated signals. This protein plays a regulatory role in initiation of cytokinesis, controlling cell growth and differentiation of hematopoietic cells, regulating spermatogenesis, and in neuronal proliferation. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Sep 2011]
Uniprot Description
MgcRacGAP: a rac GTPase activating protein. Plays an important role in cytokinesis by regulating cortical movement through RhoA. Phosphorylation by Aurora B apparently induces GAP activity toward RhoA.
Protein type: GAPs, Rac/Rho; GAPs
Chromosomal Location of Human Ortholog: 12q13.12
Cellular Component: acrosome; cleavage furrow; cytoplasm; cytosol; extrinsic to internal side of plasma membrane; microtubule; midbody; nucleoplasm; nucleus; spindle midzone
Molecular Function: alpha-tubulin binding; beta-tubulin binding; gamma-tubulin binding; GTPase activator activity; metal ion binding; microtubule binding; phosphatidylinositol-3,4,5-triphosphate binding; protein binding; protein kinase binding
Biological Process: antigen processing and presentation of exogenous peptide antigen via MHC class II; blood coagulation; cytokinesis after mitosis; cytokinesis, contractile ring formation; embryonic development; microtubule-based movement; neuroblast proliferation; positive regulation of cytokinesis; positive regulation of GTPase activity; regulation of attachment of spindle microtubules to kinetochore; regulation of small GTPase mediated signal transduction; small GTPase mediated signal transduction; spermatogenesis; spindle midzone assembly involved in mitosis; sulfate transport
Research Articles on RACGAP1
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Product Notes
The RACGAP1 racgap1 (Catalog #AAA9413633) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RACGAP1 antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's RACGAP1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). Western blotting: 1:500-1:3000 Immunohistochemistry: 1:100-1:500 Immunofluorescence: 1:100-1:200. Researchers should empirically determine the suitability of the RACGAP1 racgap1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RACGAP1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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