Rabbit anti-Human RACGAP1 Polyclonal Antibody | anti-RACGAP1 antibody

RACGAP1, NT (RACGAP1, KIAA1478, MGCRACGAP, Rac GTPase-activating protein 1, Male germ cell RacGap, Protein CYK4 homolg)

Cat. Num. AAA642388

• Gene Names: RACGAP1; CYK4; ID-GAP; HsCYK-4; MgcRacGAP
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay, Immunofluorescence
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: RACGAP1; Polyclonal Antibody; NT (RACGAP1; KIAA1478; MGCRACGAP; Rac GTPase-activating protein 1; Male germ cell RacGap; Protein CYK4 homolg); Anti -RACGAP1; anti-RACGAP1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-RACGAP1 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Immunofluorescence (IF)
• Application Notes: Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:100-500; Immunohistochemistry: 1:50-100; Immunofluorescence: 1:10-50; Flow Cytometry: 1:10-50
• Immunogen: RACGAP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 30-58 amino acids from the N-terminal region of human RACGAP1.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(RACGAP1 Antibody (N-term) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the RACGAP1 antibody detected the RACGAP1 protein (arrow).)

Immunohistochemistry (IHC)

(RACGAP1 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of RACGAP1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of RACGAP1 Antibody (N-term) with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)

Flow Cytometry (FC/FACS)

(RACGAP1 Antibody (N-term) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-RACGAP1 antibody

Rho GTPases control a variety of cellular processes. There are 3 subtypes of Rho GTPases in the Ras superfamily of small G proteins: RHO (see MIM 165370), RAC (see RAC1; MIM 602048), and CDC42 (MIM 116952). GTPase-activating proteins (GAPs) bind activated forms of Rho GTPases and stimulate GTP hydrolysis. Through this catalytic function, Rho GAPs negatively regulate Rho-mediated signals. GAPs may also serve as effector molecules and play a role in signaling downstream of Rho and other Ras-like GTPases.

Product Categories/Family for anti-RACGAP1 antibody

Antibodies; Abs to GTPase; Rab; Ras; Rho Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 186910302
• NCBI GeneID: 29127
• NCBI Accession #: NP_001119576.1
• NCBI GenBank Nucleotide #: NM_001126104.1
• UniProt Accession #: Q9H0H5; Q6PJ26; Q9NWN2; Q9P250; Q9P2W2
• Molecular Weight: 71,027 Da
• NCBI Official Full Name: rac GTPase-activating protein 1
• NCBI Official Synonym Full Names: Rac GTPase activating protein 1
• NCBI Official Symbol: RACGAP1
• NCBI Official Synonym Symbols: CYK4; ID-GAP; HsCYK-4; MgcRacGAP
• NCBI Protein Information: rac GTPase-activating protein 1; protein CYK4 homolg; protein CYK4 homolog; male germ cell RacGap; GTPase activating protein
• UniProt Protein Name: Rac GTPase-activating protein 1
• Protein Family: Rac GTPase-activating protein
• UniProt Gene Name: RACGAP1
• UniProt Synonym Gene Names: KIAA1478; MGCRACGAP; MgcRacGAP; CYK4; HsCYK-4
• UniProt Entry Name: RGAP1_HUMAN

NCBI Description

The protein encoded by this gene belongs to the GTPase-activating protein (GAP) family. GAPs bind activated forms of Rho GTPases and stimulate GTP hydrolysis. Through this catalytic function, GAPs negatively regulate Rho-mediated signals. This protein plays a regulatory role in initiation of cytokinesis, controlling cell growth and differentiation of hematopoietic cells, regulating spermatogenesis, and in neuronal proliferation. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Sep 2011]

Uniprot Description

MgcRacGAP: a rac GTPase activating protein. Plays an important role in cytokinesis by regulating cortical movement through RhoA. Phosphorylation by Aurora B apparently induces GAP activity toward RhoA.

Protein type: GAPs, Rac/Rho; GAPs

Chromosomal Location of Human Ortholog: 12q13.12

Cellular Component: nucleoplasm; microtubule; extrinsic to internal side of plasma membrane; acrosome; midbody; spindle midzone; nucleus; cytosol; cleavage furrow

Molecular Function: protein binding; gamma-tubulin binding; phosphatidylinositol-3,4,5-triphosphate binding; microtubule binding; metal ion binding; beta-tubulin binding; protein kinase binding; alpha-tubulin binding; GTPase activator activity

Biological Process: positive regulation of cytokinesis; regulation of attachment of spindle microtubules to kinetochore; spindle midzone assembly involved in mitosis; sulfate transport; antigen processing and presentation of exogenous peptide antigen via MHC class II; microtubule-based movement; embryonic development; regulation of small GTPase mediated signal transduction; cytokinesis after mitosis; small GTPase mediated signal transduction; neuroblast proliferation; spermatogenesis; blood coagulation; positive regulation of GTPase activity; cytokinesis, contractile ring formation