Goat Rabenosyn 5 Polyclonal Antibody | anti-ZFYVE20 antibody

Goat anti-Rabenosyn 5 Antibody

Cat. Num. AAA420117

• Gene Names: RBSN; ZFYVE20; Rabenosyn-5
• Reactivity: Tested: Human; Expected from sequence similarity: Human
• Applications: ELISA, Western Blot
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: Rabenosyn 5; Polyclonal Antibody; Goat anti-Rabenosyn 5 Antibody; ZFYVE20; FYVE-finger-containing Rab5 effector protein rabenosyn-5; zinc finger; FYVE domain containing 20; FLJ34993; MGC126210; Rabenosyn-5; ZFYVE20 antibody; Rabenosyn 5 antibody; FYVE-finger-containing Rab5 effector protein rabenosyn-5 antibody; FYVE domain containing 20 antibody; FLJ34993 antibody; MGC126210 antibody; Rabenosyn-5 antibody; anti-ZFYVE20 antibody

• Host: Goat
• Reactivity: Tested: Human; Expected from sequence similarity: Human
• Clonality: Polyclonal
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Concentration: 100ug specific antibody in 200ul (varies by lot)
• Sequence: ELKHTLAKQKGGTD
• Sequence Length: 784

• Applicable Applications for anti-ZFYVE20 antibody: Peptide ELISA (EIA), Western Blot (WB)
• Application Notes: Peptide ELISA: Antibody detection limit dilution 1: 64000.; Western Blot: Approx 110 kDa band observed in A431, Fibroblast (epidermoid carcinoma) lysates (calculated MW of 88.8 kDa according to NP_071735). Band migration is consistent with literature (PMID 11062261, 15020713). Recommended concentration: 0.3 - 1.5 ug/ml.
• Immunogen: Peptide with sequence C-ELKHTLAKQKGGTD, from the C Terminus of the protein sequence according to NP_071735.2.
• Epitope: C Terminus
• Preparation and Storage: Aliquot and store at -20 degree C. Minimize freezing and thawing.

Western Blot (WB)

((0.3ug/ml) staining of A431 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

NCBI and Uniprot Product Information

• NCBI GI #: 70980547
• NCBI GeneID: 64145
• NCBI Accession #: NP_071735.2
• NCBI GenBank Nucleotide #: NM_022340.3
• Molecular Weight: 23,794 Da
• NCBI Official Full Name: rabenosyn-5
• NCBI Official Synonym Full Names: rabenosyn, RAB effector
• NCBI Official Symbol: RBSN
• NCBI Official Synonym Symbols: ZFYVE20; Rabenosyn-5
• NCBI Protein Information: rabenosyn-5
• UniProt Protein Name: Rabenosyn-5
• UniProt Gene Name: RBSN
• UniProt Entry Name: RBNS5_HUMAN

NCBI Description

This gene encodes a protein that belongs to the FYVE zinc finger family of proteins. The encoded protein interacts with Ras-related proteins that regulate membrane trafficking. A missense mutation in this gene is associated with a defect in the early endocytic pathway. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014]

Uniprot Description

ZFYVE20: Rab4/Rab5 effector protein acting in early endocytic membrane fusion and membrane trafficking of recycling endosomes. Required for endosome fusion either homotypically or with clathrin coated vesicles. Plays a role in the lysosomal trafficking of CTSD/cathepsin D from the Golgi to lysosomes. Also promotes the recycling of transferrin directly from early endosomes to the plasma membrane. Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate (PtdInsP3).

Protein type: C2H2-type zinc finger protein; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 3p25.1

Cellular Component: cytosol; early endosome membrane; endosome; endosome membrane; intracellular membrane-bound organelle; plasma membrane

Molecular Function: protein binding; Rab GTPase binding; zinc ion binding

Biological Process: blood coagulation; endocytosis; endosomal vesicle fusion; endosome transport; Golgi to endosome transport; Golgi to vacuole transport; protein transport; vacuole inheritance