ADAM9 recombinant protein

Recombinant Mouse ADAM9 Protein (His tag)

Cat. Num. AAA2547184

• Gene Names: Adam9; MDC9; Mltng; AU020942; mKIAA0021
• Purity: > 87 % as determined by SDS-PAGE
• Synonyms: ADAM9; Recombinant Mouse ADAM9 Protein (His tag); AU020942; MDC9; Mltng; mKIAA0021; ADAM9 recombinant protein

• Host: Human Cells
• Purity/Purification: > 87 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 845

• Application Notes: The secreted recombinant mouse ADAM9 consists of 679 amino acids and has a calculated molecular mass of 74.9 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 25 kDa protein in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Gly 30
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for ADAM9 recombinant protein

Background: ADAM9 (A disintegrin and metallopeptidase domain 9, MDC9, meltrin gamma), is a type 1 transmembrane protein that has been associated with cancer development and metastases. ADAM9 is consistently overexpressed in various human cancers, and plays a role in tumorigenesis in mouse models. ADAM9 cleaves and releases a number of molecules with important roles in tumorigenesis and angiogenesis, such as EGF, FGFR2iiib, Tie-2, Flk-1, EphB4, CD40, VCAM-1, and VE-cadherin, and could represent a potential therapeutic target in tumors where it is highly expressed. ADAM9 belongs to a family of transmembrane, disintegrin-containing metalloproteinases involved in protein ectodomain shedding and cell-cell and cell-matrix interactions. ADAM-9 adhesive domain plays a role in regulating the motility of cells by interaction with beta1 integrins and modulates MMP synthesis.

Description: A DNA sequence encoding the mouse ADAM9 (Q61072) extracellular domain (Met 1-Asp 697) was expressed with a C-terminal polyhistidine tag.

NCBI and Uniprot Product Information

• NCBI GI #: 160358787
• NCBI GeneID: 11502
• NCBI Accession #: NP_031430.2
• NCBI GenBank Nucleotide #: NM_007404.2
• UniProt Accession #: Q61072; Q60618; Q61853; Q80U94; E9QPP2
• Molecular Weight: 92,079 Da
• NCBI Official Full Name: disintegrin and metalloproteinase domain-containing protein 9 isoform 2
• NCBI Official Synonym Full Names: a disintegrin and metallopeptidase domain 9 (meltrin gamma)
• NCBI Official Symbol: Adam9
• NCBI Official Synonym Symbols: MDC9; Mltng; AU020942; mKIAA0021
• NCBI Protein Information: disintegrin and metalloproteinase domain-containing protein 9
• UniProt Protein Name: Disintegrin and metalloproteinase domain-containing protein 9
• Protein Family: Disintegrin and metalloproteinase domain-containing protein
• UniProt Gene Name: Adam9
• UniProt Synonym Gene Names: ADAM 9

Uniprot Description

ADAM9: Probable zinc protease. May mediate cell-cell or cell- matrix interactions. Isoform 2 displays alpha-secretase activity for APP. Widely expressed. Expressed in chondrocytes. Isoform 2 is highly expressed in liver and heart. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.24.-; Membrane protein, integral; Motility/polarity/chemotaxis; Protease

Chromosomal Location of Human Ortholog: 8 A2|8 13.7 cM

Cellular Component: basolateral plasma membrane; cell surface; cytoplasm; extracellular space; focal adhesion; plasma membrane

Molecular Function: collagen binding; integrin binding; laminin binding; metalloendopeptidase activity; metallopeptidase activity; protein binding; protein kinase C binding; SH3 domain binding

Biological Process: activation of MAPKK activity; cell adhesion; cell adhesion mediated by integrin; cell migration; cell-matrix adhesion; membrane protein ectodomain proteolysis; monocyte activation; positive regulation of cell adhesion mediated by integrin; positive regulation of keratinocyte migration; positive regulation of membrane protein ectodomain proteolysis; positive regulation of protein secretion; response to calcium ion; response to glucocorticoid stimulus; response to hydrogen peroxide; response to manganese ion; transforming growth factor beta receptor signaling pathway