Western Blot (WB)
(Western Blot analysis in K562 cell line lysates (35ug/lane) using MBS624193. RAB7 (arrow) was detected using MBS624193.)
Rabbit anti-Human RAB7 Polyclonal Antibody | anti-RAB7A antibody
RAB7, CT (Ras-related Protein Rab-7a, RAB7A)
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Dilution: ELISA: 1:1,000
Western Blot: 1:100-1:500
Western Blot (WB)
(Western Blot analysis in K562 cell line lysates (35ug/lane) using MBS624193. RAB7 (arrow) was detected using MBS624193.)
Western Blot (WB)
(Western Blot analysis in K562 cell line lysates (35ug/lane) using MBS624193. RAB7 (arrow) was detected using MBS624193.)
Testing Data
(All lanes: MBS624193 (1:2000) Lane 1: A431 whole cell lysate Lane 2: C2C12 whole cell lysate Lane 3: Hela whole cell lysate Lane 4: NIH/3T3 whole cell lysate. Lysates/proteins at 20ug/lane. Secondary IgG (HRP) GxRt Predicted band size: 23kD Blocking/Dilution buffer: 5% NFDM/TBST.)
Testing Data
(All lanes: MBS624193 (1:2000) Lane 1: A431 whole cell lysate Lane 2: C2C12 whole cell lysate Lane 3: Hela whole cell lysate Lane 4: NIH/3T3 whole cell lysate. Lysates/proteins at 20ug/lane. Secondary IgG (HRP) GxRt Predicted band size: 23kD Blocking/Dilution buffer: 5% NFDM/TBST.)
Immunohistochemistry (IHC)
(Immunohistochemistry staining of RAB7 in human skeletal muscle tissue sections using MBS624193 Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5hr at RT; antigen retrieval was by heat mediation with a citrate buffer (pH 6). Samples were incubated with MBS624193 (1:25) for 1hr at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.)
Immunohistochemistry (IHC)
(Immunohistochemistry staining of RAB7 in human skeletal muscle tissue sections using MBS624193 Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5hr at RT; antigen retrieval was by heat mediation with a citrate buffer (pH 6). Samples were incubated with MBS624193 (1:25) for 1hr at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis in formalin-fixed and paraffin-embedded human skeletal muscle reacted with MBS624193, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of MBS624193 for immunohistochemistry.)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis in formalin-fixed and paraffin-embedded human skeletal muscle reacted with MBS624193, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of MBS624193 for immunohistochemistry.)
NCBI and Uniprot Product Information
NCBI Description
RAB family members are small, RAS-related GTP-binding proteins that are important regulators of vesicular transport. Each RAB protein targets multiple proteins that act in exocytic / endocytic pathways. This gene encodes a RAB family member that regulates vesicle traffic in the late endosomes and also from late endosomes to lysosomes. This encoded protein is also involved in the cellular vacuolation of the VacA cytotoxin of Helicobacter pylori. Mutations at highly conserved amino acid residues in this gene have caused some forms of Charcot-Marie-Tooth (CMT) type 2 neuropathies. [provided by RefSeq]
Uniprot Description
Rab7: a member of the RAB family of RAS-related GTP-binding proteins, important regulators of vesicular transport and are located in specific intracellular compartments. RAB7 has been localized to late endosomes and shown to be important in the late endocytic pathway. In addition, it has been shown to have a fundamental role in the cellular vacuolation induced by the cytotoxin VacA of Helicobacter pylori. Mutations in this protein cause Charcot-Marie-Tooth type 2B neuropathy.
Protein type: G protein, monomeric; G protein; G protein, monomeric, Rab
Chromosomal Location of Human Ortholog: 3q21.3
Cellular Component: Golgi apparatus; phagocytic vesicle membrane; intracellular membrane-bound organelle; lysosomal membrane; retromer complex; lysosome; late endosome; vacuolar membrane; pre-autophagosomal structure membrane; melanosome; phagocytic vesicle
Molecular Function: GTPase activity; protein binding; GDP binding; GTP binding; Rac GTPase binding
Biological Process: protein transport; protein targeting to lysosome; early endosome to late endosome transport; endosome to lysosome transport; antigen processing and presentation of exogenous peptide antigen via MHC class II; endocytosis; protein to membrane docking; Rab protein signal transduction; retrograde transport, endosome to Golgi; epidermal growth factor ligand processing; bone resorption
Disease: Charcot-marie-tooth Disease, Axonal, Type 2b
Research Articles on RAB7A
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Product Notes
The RAB7A rab7a (Catalog #AAA624193) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RAB7, CT (Ras-related Protein Rab-7a, RAB7A) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's RAB7 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in ELISA and Western Blot. Dilution: ELISA: 1:1,000 Western Blot: 1:100-1:500. Researchers should empirically determine the suitability of the RAB7A rab7a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RAB7, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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