Western Blot (WB)
(Anti-RAB5B rabbit polyclonal antibody at 1:500 dilution Lane A: HeLa Whole Cell Lysate Lane B: Jurkat Whole Cell Lysate Lane C: HEK293 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:23 kDa Observed band size:23 kDa)
Rabbit RAB5B Polyclonal Antibody | anti-RAB5B antibody
Anti-RAB5B Antibody, Rabbit Polyclonal
Reactivity
HumanPredicted to React with: Mouse, Pongo abelii (Species predicted to react based on 100% sequence homology)
Applications
Western Blot
Purity
Protein A & Antigen Affinity
Synonyms
RAB5B; Polyclonal Antibody; Anti-RAB5B Antibody; Rabbit Polyclonal; RAB5B Antibody; Rabbit PAb; Antigen Affinity Purified; member RAS oncogene family; anti-RAB5B antibody
Host
Rabbit
Reactivity
Human
Predicted to React with: Mouse, Pongo abelii (Species predicted to react based on 100% sequence homology)
Predicted to React with: Mouse, Pongo abelii (Species predicted to react based on 100% sequence homology)
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human RAB5B
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; PBS, pH7.0 with 0.03% Proclin300
Applicable Applications for anti-RAB5B antibody
Western Blot (WB)
Application Notes
WB: 1:500-1:2000
Immunogen
A synthetic peptide corresponding to the C-terminus of the Human RAB5B
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human RAB5B, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Anti-RAB5B rabbit polyclonal antibody at 1:500 dilution Lane A: HeLa Whole Cell Lysate Lane B: Jurkat Whole Cell Lysate Lane C: HEK293 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:23 kDa Observed band size:23 kDa)
Western Blot (WB)
(Anti-RAB5B rabbit polyclonal antibody at 1:500 dilution Lane A: HeLa Whole Cell Lysate Lane B: Jurkat Whole Cell Lysate Lane C: HEK293 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:23 kDa Observed band size:23 kDa)
Related Product Information for anti-RAB5B antibody
Antisense inhibition of Rab5b, a gene coding for a small GTPase associated with endocytosis, significantly reduced the mGluR-mediated neuroprotection. Ras-related protein Rab-5B (RAB5B), which is identified by a genome-wide association study as a risk locus for this syndrome, encodes a small GTPase involved in the control of receptor internalization and early endosome fusion. Previous genome-wide sequencing revealed that RAB5B is a susceptible target in patients with polycystic ovary syndrome (PCOS). RAB5A gene was abnormally expressed in luteinized granulosa cells of obese patients with polycystic ovary syndrome, which may help explain high FSHR levels found in this syndrome. RAB5B is directly downregulated by miR-130a-3p. Knockdown of RAB5B also inhibited cell proliferation, migration, and invasion.
Product Categories/Family for anti-RAB5B antibody
References
Zhu K, et al. (2018) Role of rab5a in fshr-mediated signal transduction in human granulosa cells. Reproduction 155 (6): 505-514.Yu J, et al. (2019) Association of single nucleotide polymorphisms in the rab5b gene 3'utr region with polycystic ovary syndrome in chinese han women. Biosci Rep 39 (5): BSR20190292.Baskys A, et al. (2005) Activation of neuroprotective pathways by metabotropic group i glutamate receptors: A potential target for drug discovery? Ann N Y Acad Sci 1053 55-73.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
215
NCBI Official Full Name
Ras-related protein Rab-5B
NCBI Official Synonym Full Names
RAB5B, member RAS oncogene family
NCBI Official Symbol
RAB5B
NCBI Protein Information
ras-related protein Rab-5B
UniProt Protein Name
Ras-related protein Rab-5B
Protein Family
UniProt Gene Name
RAB5B
UniProt Entry Name
RAB5B_HUMAN
Uniprot Description
RAB5B: Protein transport. Probably involved in vesicular traffic. Belongs to the small GTPase superfamily. Rab family.
Protein type: G protein; G protein, monomeric, Rab; G protein, monomeric
Chromosomal Location of Human Ortholog: 12q13
Cellular Component: membrane; endocytic vesicle; early endosome membrane; early endosome; plasma membrane; melanosome; intracellular; endosome
Molecular Function: GTPase activity; protein binding; GDP binding; GTP binding; GTP-dependent protein binding
Biological Process: antigen processing and presentation; intracellular protein transport; endosome organization and biogenesis; metabolic process; regulation of endocytosis; plasma membrane to endosome transport; Rab protein signal transduction
Research Articles on RAB5B
Similar Products
Product Notes
The RAB5B rab5b (Catalog #AAA8104064) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-RAB5B Antibody, Rabbit Polyclonal reacts with Human Predicted to React with: Mouse, Pongo abelii (Species predicted to react based on 100% sequence homology) and may cross-react with other species as described in the data sheet. AAA Biotech's RAB5B can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:2000. Researchers should empirically determine the suitability of the RAB5B rab5b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RAB5B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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