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Western Blot (WB) (Western Blot;Sample: Human Urine;Primary Ab: 1ug/ml Rabbit Anti-Human RNASE2 AntibodySecond Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: MBS2086047 ))

Rabbit Ribonuclease A2 (RNASE2) Polyclonal Antibody | anti-RNASE2 antibody

Ribonuclease A2 (RNASE2) Polyclonal Antibody

Gene Names
RNASE2; EDN; RAF3; RNS2
Reactivity
Homo sapiens (Human)
Applications
Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Synonyms
Ribonuclease A2 (RNASE2); Polyclonal Antibody; Ribonuclease A2 (RNASE2) Polyclonal Antibody; Polyclonal Antibody to Ribonuclease A2 (RNASE2); Rnase-A2; Rnase2; EDN; Ribonuclease US; Ribonuclease; RNase A Family; 2; Liver; Eosinophil-Derived Neurotoxin; MNon-secretory ribonuclease; anti-RNASE2 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Homo sapiens (Human)
Clonality
Polyclonal
Purity/Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Form/Format
Supplied as solution form in 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Concentration
0.93mg/ml (varies by lot)
Applicable Applications for anti-RNASE2 antibody
Western Blot (WB); Immunohistochemistry (IHC); Immunocytochemistry (ICC); Immunoprecipitation (IP).
Application Notes
Western blotting: 0.5-2ug/mL
Immunohistochemistry: 5-20ug/mL
Immunocytochemistry: 5-20ug/mL
Optimal working dilutions must be determined by end user.
Source
Polyclonal antibody preparation
Traits
Liquid
Cross Reactivity
Mouse, Pig
Immunogen
Recombinant RNASE2 (Lys28~Ile161) expressed in E.coli ( MBS2097579 ).
Preparation and Storage
Storage:
Avoid repeated freeze/thaw cycles.
Store at 4 degree C for frequent use.
Aliquot and store at -20 degree C for 24 months.

Stability Test:
thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Western Blot (WB)

(Western Blot;Sample: Human Urine;Primary Ab: 1ug/ml Rabbit Anti-Human RNASE2 AntibodySecond Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: MBS2086047 ))

Western Blot (WB) (Western Blot;Sample: Human Urine;Primary Ab: 1ug/ml Rabbit Anti-Human RNASE2 AntibodySecond Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: MBS2086047 ))

Immunohistochemistry (IHC)

(DAB staining on IHC-P;Samples: Human Kidney Tissue;Primary Ab: 10ug/ml Rabbit Anti-Human RNASE2 AntibodySecond Ab: 2ug/mL HRPLinked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: MBS2086047 ))

Immunohistochemistry (IHC) (DAB staining on IHC-P;Samples: Human Kidney Tissue;Primary Ab: 10ug/ml Rabbit Anti-Human RNASE2 AntibodySecond Ab: 2ug/mL HRPLinked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: MBS2086047 ))

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
non-secretory ribonuclease
NCBI Official Synonym Full Names
ribonuclease A family member 2
NCBI Official Symbol
RNASE2
NCBI Official Synonym Symbols
EDN; RAF3; RNS2
NCBI Protein Information
non-secretory ribonuclease
UniProt Protein Name
Non-secretory ribonuclease
UniProt Gene Name
RNASE2
UniProt Synonym Gene Names
EDN; RNS2; RNase 2

NCBI Description

The protein encoded by this gene is a non-secretory ribonuclease that belongs to the pancreatic ribonuclease family, a subset of the ribonuclease A superfamily. The protein antimicrobial activity against viruses. [provided by RefSeq, Oct 2014]

Uniprot Description

RNASE2: This is a non-secretory ribonuclease. It is a pyrimidine specific nuclease with a slight preference for U. Cytotoxin and helminthotoxin. Selectively chemotactic for dendritic cells. Possesses a wide variety of biological activities. Belongs to the pancreatic ribonuclease family.

Protein type: EC 3.1.27.5; Motility/polarity/chemotaxis; RNA processing; Ribonuclease

Chromosomal Location of Human Ortholog: 14q11.2

Cellular Component: extracellular region

Molecular Function: endonuclease activity; lipopolysaccharide binding; nucleic acid binding; ribonuclease A activity; ribonuclease activity

Biological Process: chemotaxis; defense response to Gram-negative bacterium; defense response to Gram-positive bacterium; defense response to virus; induction of bacterial agglutination; neutrophil degranulation; RNA catabolic process

Research Articles on RNASE2

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Product Notes

The RNASE2 rnase2 (Catalog #AAA2090429) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Ribonuclease A2 (RNASE2) Polyclonal Antibody reacts with Homo sapiens (Human) and may cross-react with other species as described in the data sheet. AAA Biotech's Ribonuclease A2 (RNASE2) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB); Immunohistochemistry (IHC); Immunocytochemistry (ICC); Immunoprecipitation (IP). Western blotting: 0.5-2ug/mL Immunohistochemistry: 5-20ug/mL Immunocytochemistry: 5-20ug/mL Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the RNASE2 rnase2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Ribonuclease A2 (RNASE2), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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