Goat Rab5b Polyclonal Antibody | anti-Rab5b antibody

Rab5b Polyclonal Antibody

Cat. Num. AAA448063

• Reactivity: Reacts against: Human, Rat, Mouse, Canine, and Monkey proteins
• Applications: Immunofluorescence, Western Blot, Immunohistochemistry, Immunohistochemistry
• Synonyms: Rab5b; Polyclonal Antibody; Rab5b Polyclonal Antibody; Anti-Rab5b; RAB5B; RAS-associated protein RAB5B; ras-related protein Rab-5B antibody; anti-Rab5b antibody

• Host: Goat
• Reactivity: Reacts against: Human, Rat, Mouse, Canine, and Monkey proteins
• Clonality: Polyclonal
• Specificity: Detects Rab5b protein by Western blot in cell lysates of transfected cells with GFP-Rab5b. This Ab is specific for Rab5b, it does not recognise Rab5a and Rab5c.
• Form/Format: Polyclonal antibody supplied as 200ul (4 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.
• Sequence Length: 215

• Applicable Applications for anti-Rab5b antibody: Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry-(Paraffin) (IHC-P), Immunohistochemistry-(Frozen) (IHC-F)
• Application Notes: WB: 1:250 - 1:1,000; IF: 1:50 - 1:250
• Immunogen: Purified recombinant peptide derived from within residues 115 aa to the C-terminus of mouse Rab5b produced in E. coli.
• Preparation and Storage: Store at -20°C for long term storage. Store at 2-8°C for up to one month. Avoid freeze/thaw cycles.

Reactivity Chart

Testing Data

Related Product Information for anti-Rab5b antibody

RAB5B, member RAS oncogene family

Goat polyclonal antibody to mouse Rab5b. Rab5b belongs to the small GTPase superfamily, Rab family. Rab5b is a Early Endosome Marker and functions as a key regulator of vesicular trafficking during early endocytosis.

NCBI and Uniprot Product Information

• NCBI GI #: 49456341
• NCBI GeneID: 5869
• UniProt Accession #: P61020; P35239; P35277; Q6PIK9; Q86TH0; Q8IXL2; A8K982; B4DKD7
• NCBI Official Full Name: RAB5B
• NCBI Official Synonym Full Names: RAB5B, member RAS oncogene family
• NCBI Official Symbol: RAB5B
• NCBI Protein Information: ras-related protein Rab-5B
• UniProt Protein Name: Ras-related protein Rab-5B
• Protein Family: Ras-related protein
• UniProt Gene Name: RAB5B
• UniProt Entry Name: RAB5B_HUMAN

Uniprot Description

Function: Protein transport. Probably involved in vesicular traffic

By similarity.

Enzyme regulation: Regulated by guanine nucleotide exchange factors (GEFs) which promote the exchange of bound GDP for free GTP.

Subunit structure: Binds EEA1. Interacts with RIN2 and RIN3, which probably regulate its pathway, possibly by acting as GEFs. Ref.8 Ref.9

Subcellular location: Cell membrane; Lipid-anchor; Cytoplasmic side. Early endosome membrane; Lipid-anchor. Melanosome. Note: Enriched in stage I melanosomes. Ref.10

Sequence similarities: Belongs to the small GTPase superfamily. Rab family.

Sequence caution: The sequence AAH40143.1 differs from that shown. Reason: Erroneous initiation.